PXD043912

PXD043912 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics analysis of different sources of hMSCs
Description	Mesenchymal stem cells (MSCs) are an attractive therapeutic tool for tissue engineering and re-generative medicine due to their regenerative and trophic properties. The best-known and most widely used are bone marrow MSCs, but they are now being harvested and developed from a wide range of adult and perinatal tissues. MSCs from different sources are believed to have dif-ferent secretion potentials and production, which may influence their therapeutic effects. To prove it, we performed a quantitative proteomic analysis based on the TMT technique of MSCs from 3 different sources: wharton’s jelly (WJ), dental pulp (DP) and bone marrow (BM). Our analysis has focused on MSC biological properties of interest for tissue engineering. We identified a total of 611 human proteins differentially expressed. WJ-MSCs shown the greatest variation compared to the other sources. WJ produced more extra-cellular matrix (ECM) proteins and ECM-affiliated proteins and appeared to more able to modulate the inflammatory and immune response. BM-MSCs display enhanced differentiation and paracrine communication capabilities. DP-MSC appeared to promote exosome production. The results obtained confirm the existence of differences between WJ, DP and BM-MSC and the need to select the MSC origin according to the therapeutic objective sought.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:54.706.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043912
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paulo MARCELO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-19 05:49:49	ID requested
1	2023-09-20 00:55:43	announced
⏵ 2	2023-11-14 09:02:55	announced	2023-11-14: Updated project metadata.

10.6019/PXD043912;

submitter keyword: proteomics,mesenchymal stem cells, tissue engineering, regenerative medicine

Paulo MARCELO
contact affiliation	University of Picardie Jules Verne
contact email	paulo.marcelo@u-picardie.fr
lab head
Paulo MARCELO
contact affiliation	Université de Picardie Jules Verne
contact email	paulo.marcelo@u-picardie.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043912
     1. Label: PRIDE project
     2. Name: Quantitative proteomics analysis of different sources of hMSCs