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A Cell Culture Model of Resistance Arteries
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A Cell Culture Model of Resistance Arteries

Article DOI: 10.3791/55992-v • 10:54 min • September 8th, 2017
September 8th, 2017 •
1,2, 3, 4, 3, 1,2
1Department of Molecular Physiology and Biophysics, University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine, 3Department of Hematology, St. Jude Children's Research Hospital, 4Research Histology Core, University of Virginia School of Medicine

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Summary
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A cell culture model of resistance arteries is described, allowing for the dissection of signaling pathways in endothelium, smooth muscle, or between endothelium and smooth muscle (the myoendothelial junction). The selective application of agonists or protein isolation, electron microscopy, or immunofluorescence can be utilized using this cell culture model.

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Cell Culture Model Resistance Arteries Endothelial Cells Smooth Muscle Cells In-vitro Culture Myoendothelial Junctions Cardiovascular Field Protein Localization Signaling Cascades Arterial Wall Myoendothelial Junction Fractions Paraffin Embedding Vascular Cell Co-culture VCCC Disinfectant Petri Dishes Fiberactin Solution
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