Login Register Cart 0
Generic placeholder image

Recent Patents on Food, Nutrition & Agriculture

Editor-in-Chief

ISSN (Print): 2212-7984
ISSN (Online): 1876-1429

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
General Research Article

Chemical Constituents, Antioxidant, Anticholinesterase and Antiproliferative Effects of Algerian Pistacia atlantica Desf. Extracts

Author(s): Imene Achili, Amel Amrani, Chaouki Bensouici, Fatih Gül, Muhammed Altun, Ibrahim Demirtas, Djamila Zama, Fadila Benayache and Samir Benayache*

Volume 11, Issue 3, 2020

Page: [249 - 256] Pages: 8

DOI: 10.2174/2212798411666200207101502

Abstract

Background: Pistacia atlantica Desf. (Anacardiaceae) has various applications for dietetic and medicinal purposes.

Objective: The aim of the present study was to evaluate antioxidant, antiproliferative and anticholinesterase activities of different extracts from leaf and stem of Pistacia atlantica Desf.

Methods: The antioxidant activity was performed by four methods: DPPH, ABTS, CUPRAC and reducing power assays. Anti-cholinesterase activity was performed against acetylcholinesterase (AChE) and Butyrylcholinesterase (BuChE) enzymes. Antiproliferative assays were investigated against HeLa cell lines using xCELLigence RTCA instrument. The secondary metabolites composition was established by HPLC-TOF/MS analysis.

Results: In DPPH, reducing power and in ABTS .+ scavenging activity, all the extracts showed strong inhibitory activity compared to synthetic antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA), in which the activities were almost equal to the two standards. The results were less significant in CUPRAC assay. The ethyl acetate (EtOAc) extracts exhibited the best antioxidant activity in all tests. Moreover, P. atlantica extracts inhibited AChE and BChE activities in a dose-dependent manner. The strongest AChE and BuChE inhibition activities were obtained for EtOAc extract of the stem (IC50 values 15.14±0.74 and 24.01±0.21 μg/mL, respectively) compared to galantamine (IC50 values 6.27±1.15 and 34.75±1.99 μg/mL, respectively). P. atlantica extracts also showed significant antiproleferative activity against HeLa cell lines, the best antiproleferative activity was obtained for the methanol and EtOAc extracts. The observed biological activities can be attributed to the presence of phenolic compounds and flavonoids in the extracts. The HPLC-TOF/MS analysis identified the presence of 22 phytochemicals. Gallic acid and rutin were the main compounds detected. Cichoric, gentisic, vanillic, protocatechuic and rosmarinic acids as well as catechin and quercetin were also present.

Conclusion: This study demonstrated good antioxidant, anticholinesterase and antiproliferative activities of P. atlantica extracts, which opens up new possibilities for pharmaceutical and food industries.

Keywords: Pistacia atlantica Desf., phenolic compounds, flavonoids, antioxidant, antiproliferative, anticholinesterase activities.

« Previous Next »
Graphical Abstract

[1]
Thanan R, Oikawa S, Hiraku Y, et al. Oxidative stress and its significant roles in neurodegenerative diseases and cancer. Int J Mol Sci 2014; 16(1): 193-217.
[http://dx.doi.org/10.3390/ijms16010193] [PMID: 25547488]
[2]
Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010; 4(8): 118-26.
[http://dx.doi.org/10.4103/0973-7847.70902] [PMID: 22228951]
[3]
Mahjoub F, Akhavan Rezayat K, Yousefi M, Mohebbi M, Salari R. Pistacia atlantica Desf. A review of its traditional uses, phytochemicals and pharmacology. J Med Life 2018; 11(3): 180-6.
[http://dx.doi.org/10.25122/jml-2017-0055] [PMID: 30364651]
[4]
Belhadj S. Geographical distribution of Pistacia atlantica Desf. in Algeria. Acta Hortic 2002; (591): 499-503.
[http://dx.doi.org/10.17660/ActaHortic.2002.591.75]
[5]
Benhammou N, Bekkara FA, Panovska TK. Antioxidant and antimicrobial activities of the Pistacia lentiscus and Pistacia atlantica extracts. Afr J Pharm Pharmacol 2008; 2(2): 22-8.
[6]
Ben Ahmed Z, Yousfi M, Viaene J, et al. Antioxidant activities of Pistacia atlantica extracts modeled as a function of chromatographic fingerprints in order to identify antioxidant markers. Microchem J 2016; 128: 208-17.
[http://dx.doi.org/10.1016/j.microc.2016.04.023]
[7]
Hashemnia M, Nikousefat Z, Yazdani-Rostam M. Antidiabetic effect of Pistacia atlantica and Amygdalus scoparia in streptozotocin-induced diabetic mice. Comp Clin Pathol 2015; 24(6): 1301-6.
[http://dx.doi.org/10.1007/s00580-015-2068-1]
[8]
Hashemi L, Asadi-Samani M, Moradi MT, Alidadi S. Anticancer activity and phenolic compounds of Pistacia atlantica Extract. Int J Pharm Phyto Res 2017; 7(2): 26-31.
[9]
Bozorgi M, Memariani Z, Mobli M, Salehi Surmaghi MH, Shams-Ardekani MR, Rahimi R. Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk and P. lentiscus): a review of their traditional uses, phytochemistry, and pharmacology. ScientificWorldJournal 2013.2013219815
[http://dx.doi.org/10.1155/2013/219815] [PMID: 24453812]
[10]
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181: 1199-200.
[http://dx.doi.org/10.1038/1811199a0]
[11]
Apak R, Güçlü K, Ozyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 2004; 52(26): 7970-81.
[http://dx.doi.org/10.1021/jf048741x] [PMID: 15612784]
[12]
Oyaizu M. Studies on products of browning reaction: antioxidative activity of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44: 307-15.
[http://dx.doi.org/10.5264/eiyogakuzashi.44.307]
[13]
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26(9-10): 1231-7.
[http://dx.doi.org/10.1016/S0891-5849(98)00315-3] [PMID: 10381194]
[14]
Öztürk M, Duru ME, Kivrak S, Mercan-Doğan N, Türkoglu A, Özler MA. In vitro antioxidant, anticholinesterase and antimicrobial activity studies on three Agaricus species with fatty acid compositions and iron contents: a comparative study on the three most edible mushrooms. Food Chem Toxicol 2011; 49(6): 1353-60.
[http://dx.doi.org/10.1016/j.fct.2011.03.019] [PMID: 21419821]
[15]
Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88-95.
[http://dx.doi.org/10.1016/0006-2952(61)90145-9] [PMID: 13726518]
[16]
Boussaha S, Bekhouche K, Boudjerda A, et al. Chemical constituents, in vitro antioxidant and antiproliferative activities of Perralderia coronopifolia Coss. Subsp. eu-coronopifolia M. var. typica M. extract. Rec Nat Prod 2015; 9(3): 312-22.
[17]
Koldaş S, Demirtas I, Ozen T, Demirci MA, Behçet L. Phytochemical screening, anticancer and antioxidant activities of Origanum vulgare L. ssp. viride (Boiss.) Hayek, a plant of traditional usage. J Sci Food Agric 2015; 95(4): 786-98.
[http://dx.doi.org/10.1002/jsfa.6903] [PMID: 25200133]
[18]
Asgharia B, Zenginb G, Bahadoric MB, Abbas-Mohammadid M, Dinparaste L. Amylase, glucosidase, tyrosinase, and cholinesterases inhibitory, antioxidant effects, and GC-MS analysis of wild mint (Mentha longifolia var. calliantha) essential oil: A natural remedy. Eur J Integr Med 2018; 22: 44-9.
[http://dx.doi.org/10.1016/j.eujim.2018.08.004]
[19]
Vinutha B, Prashanth D, Salma K, et al. Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. J Ethnopharmacol 2007; 109(2): 359-63.
[http://dx.doi.org/10.1016/j.jep.2006.06.014] [PMID: 16950584]
[20]
Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008; 4(2): 89-96.
[PMID: 23675073]
[21]
Prakash D, Upadhyay G, Pushpangadan P, Gupta C. Antioxidant and free radical scavenging activities of some fruits. J Complement Integr Med 2011; 8(1)
[http://dx.doi.org/10.2202/1553-3840.1513] [PMID: 22754941]
[22]
Peksel A. Antioxidative properties of decoction of Pistacia atlantica Desf. leaves. Asian J Chem 2008; 20(1): 681-93.
[23]
Toul F, Belyagoubi-Benhammou N, Zitouni A, Atik-Bekkara F. Antioxidant activity and phenolic profile of different organs of Pistacia atlantica Desf. subsp. atlantica from Algeria. Nat Prod Res 2017; 31(6): 718-23.
[http://dx.doi.org/10.1080/14786419.2016.1217205] [PMID: 27531231]
[24]
Amri O, Zekhnini A, Bouhaimi A, Tahrouch S, Hatimi A. Anti-inflammatory activity of methanolic extract from Pistacia atlantica Desf. leaves. Pharmacogn J 2018; 10(1): 71-6.
[http://dx.doi.org/10.5530/pj.2018.1.14]
[25]
Hamaguchi T, Ono K, Murase A, Yamada M. Phenolic compounds prevent Alzheimer’s pathology through different effects on the amyloid-beta aggregation pathway. Am J Pathol 2009; 175(6): 2557-65.
[http://dx.doi.org/10.2353/ajpath.2009.090417] [PMID: 19893028]
[26]
Peksel A, Arisan-Atac I, Yanardag R. Evaluation of antioxidant and antiacetylcholinesterase activities of the extracts of Pistacia atlantica Desf. Leaves. J Food Biochem 2010; 34(3): 451-76.
[http://dx.doi.org/10.1111/j.1745-4514.2009.00290.x]
[27]
Benamar H, Marouf A, Bennaceur M. Phytochemical composition, antioxidant and acetylcholinesterase inhibitory activities of aqueous extract and fractions of Pistacia atlantica subsp. atlantica from Algeria. J Herbs Spices Med Plants 2018; 24(3): 229-44.
[http://dx.doi.org/10.1080/10496475.2018.1446204]
[28]
Orhan IE, Guner E, Ozturk N, et al. Enzyme inhibitory and antioxidant activity of Melia azedarach L. naturalized in Anatolia and its phenolic acid and fatty acid composition. Ind Crops Prod 2012; 37(1): 213-8.
[http://dx.doi.org/10.1016/j.indcrop.2011.11.028]
[29]
Pacifico S, Piccolella S, Marciano S, et al. LC-MS/MS profiling of a mastic leaf phenol enriched extract and its effects on H2O2 and Aβ(25-35) oxidative injury in SK-B-NE(C)-2 cells. J Agric Food Chem 2014; 62(49): 11957-66.
[http://dx.doi.org/10.1021/jf504544x] [PMID: 25405583]
[30]
Rayan A, Raiyn J, Falah M. Nature is the best source of anticancer drugs: Indexing natural products for their anticancer bioactivity. PLoS One 2017; 12(11)e0187925
[http://dx.doi.org/10.1371/journal.pone.0187925] [PMID: 29121120]
[31]
Prakash O, Usmani S, Gupta A, Singh R, Singh N, Ved A. Bioactive Polyphenols as promising natural medicinal agents against cancer: the emerging trends and prospective goals. Curr Bioact Compd 2018; 14.
[http://dx.doi.org/10.2174/1573407214666181030122046]
[32]
Rahman HS. Phytochemical analysis and antioxidant and anticancer activities of mastic gum resin from Pistacia atlantica subspecies kurdica. OncoTargets Ther 2018; 11: 4559-72.
[http://dx.doi.org/10.2147/OTT.S170827] [PMID: 30122948]

Rights & Permissions Print Cite
Article Metrics
69
1
© 2024 Bentham Science Publishers | Privacy Policy