Development of Yeast Molecular Display Systems Focused on Therapeutic Proteins, Enzymes, and Foods: Functional Analysis of Proteins and its Application to Bioconversion

Seiji      Shibasaki; Mitsuyoshi      Ueda

doi:10.2174/187220810793611545

Abstract

Molecular display systems using yeast have been developed for industrial, medical, pharmaceutical, and biological studies. Although several host cells are available to construct a molecular display system, the yeast Saccharomyces cerevisiae is a well-established and convenient organism in eukaryotes. A wide variety of prokaryotic and eukaryotic proteins have been displayed on yeast cell surfaces. In addition, functional analyses and applications to bioconversion have been performed on the cell surface, and cells are conveniently engineered by molecular display systems. In this review, we focus on the yeast molecular display system with regard to therapeutic proteins, several enzymes, and food ingredients. In addition, recent patents on molecular display using yeast cell for production of those compounds, screening technology and related techniques are introduced. Development of devices for functional analysis of created and modified proteins in the yeast display system is also described.

Keywords: Yeast, Molecular Display, Antibody, β-Glucosidase, Lipase, Isoflavon, Cell Chip, cytosol, immune system, T cells, B cells, ligand binding, biotechnological, antibodies, enzymes, analytical systems, cell wall, linked glucans, non-covalent bonds, inositol phospholipids, exocytosis, enclosed vesicles, Golgi apparatus, proteolytic, flocculation, PHARMACEUTI-CAL DEVELOPMENT, horseradish peroxidase, strain, pH, galactose, microscopy, fluorescence, Rhizopus oryzae, immunosuppressants, novel, mutagenesis, autolysis, stationary-phase, com-plementarity-determining regions, rate-limiting dissociation, catalytic, genome, metalloendopeptidase, neurolysin, matrix metal-loproteinases, cholinergic circuits, cell progression, pathology, pharmacology, biochemistry, Angiotensin, bradykinin, bioactive peptides, white biotechnology, emulsion, triolein, L-lactic acid, viscosity, Biodegradation, polyether-based, polyurethane, acetone, osteoporosis, post-menopausal symptoms, aglycones, fungi, mass spectrometry, hydrolysis, Fatty acids provide, volatile, esterification, oral antigen, Pharmacopeia, Pepsin, hydrogel systems, auxotrophic