Abstract
The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.
Keywords: Full-length cDNA, EST, SAGE, LongSAGE, SuperSAGE, transcriptome
Current Pharmaceutical Biotechnology
Title: Long-Short-Long Games in mRNA Identification: The Length Matters
Volume: 9 Issue: 5
Author(s): San Ming Wang
Affiliation:
Keywords: Full-length cDNA, EST, SAGE, LongSAGE, SuperSAGE, transcriptome
Abstract: The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.
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Cite this article as:
Wang Ming San, Long-Short-Long Games in mRNA Identification: The Length Matters, Current Pharmaceutical Biotechnology 2008; 9 (5) . https://dx.doi.org/10.2174/138920108785915166
DOI https://dx.doi.org/10.2174/138920108785915166 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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