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Current Pharmaceutical Biotechnology

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ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

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General Research Article

Ezetimibe Prevents IL-1β-induced Inflammatory Reaction in Mouse Chondrocytes via Modulating NF-κB and Nrf2/HO-1 Signaling Crosstalk

Author(s): Qiuyan Weng, Tongzhou Hu, Xiaohan Shen, Jinming Han, Yong Zhang* and Jianning Luo*

Volume 23, Issue 14, 2022

Published on: 29 April, 2022

Page: [1772 - 1780] Pages: 9

DOI: 10.2174/1389201023666220104141521

Price: $65

Abstract

Background: Osteoarthritis is a type of age-related, chronic, and degenerative joint disease. Ezetimibe, a cholesterol absorption inhibitor, is widely used for the treatment of various diseases. However, the role of ezetimibe in osteoarthritis remains unclear.

Objectives: This study aimed to explore the anti-inflammation effect of ezetimibe on mouse chondrocytes.

Methods: In the present study, ELISA, qPCR and western blot analysis were performed to evaluate the anti-inflammatory effects of ezetimibe. In addition, enzymes that are highly associated with the anabolism and catabolism of the extracellular matrix of the articular cartilage were also evaluated.

Results: Treatment with ezetimibe attenuated the IL-1β-induced degradation of the extracellular matrix, including aggrecan and collagen II. Ezetimibe also attenuated the IL-1β-induced expression levels of MMP3, MMP13 and ADAMTS5, thus exerting protective effects against IL-1β- induced extracellular matrix degradation. The complex mechanism of the anti-inflammatory reaction contributed to the activation of the Nrf2/HO-1 pathway and the suppression of the NF-κB pathway.

Conclusion: On the whole, the present study demonstrates that ezetimibe may be a promising agent for further osteoarthritis therapy.

Keywords: Ezetimibe, osteoarthritis, IL-1β, Nrf2/HO-1, NF-κB, chondrocytes.

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