Indian Journal of Science and Technology

Abstract

Background: Fruits and vegetables rich in antioxidant compounds which scavenges free radicals. In this research, the hepatoprotective effect of cherry fruit aqueous extracts was studied. Methods: Fruits of the cherry were collected; the seeds were removed and extracted by maceration method. For in vitro antioxidant activity, Diphenylpicryl Hydrazyl (DPPH), Trolex Equivalent Antioxidants Capacity (TEAC) and Ferric Reducing Antioxidant Power (FRAP) was carried out. Total phenol and flavonoid contents were also determined. For in vivo study, 28 wistar male rats were prepared and divided into negative control, plant control, carbon tetrachloride control (toxin) and treatment groups. At the end of 14 days, for evaluation of hepatoprotective effects of extracts, animals were exsanguinated by diethyl ether anesthesia. Blood samples were collected by heart puncture and the serum was used for assay of Aspartate Transaminase (AST), Alanine Transaminase (ALT), Alkaline Phosphatase (ALP), Total Bilirubin (TB) and albumin. All results are expressed as mean ± Standard Deviation (SD). Statistical analysis was calculated by one way analysis of variance (ANOVA). Results: Total phenol and Flavonoid contents were reported 930 mg/kg GAE and 380 mg Rutine in one Kg of fresh fruit respectively. In vitro antioxidant activity of extract in FRAP 4.8, DPPH 9.3 Mm trolox and TEAC 2.8 /kg fresh fruit were reported. There was a significant increase in hepatic enzymes activities and bilirubin level in carbon tetrachloride group compare to negative control. The extraction of cherry in 500 mg dose was able to alleviate the induced damages compare to toxic group (p<0.05). Conclusion: Cherry extract shows hepato-protective potential in selected dose.
Keywords: Antioxidant Activity, Carbon Tetrachloride, Cherry Extraction, Hepato-Protective