| 高媛,孙牧笛,徐全智,吕茜,张庆宸,顾沛雯.苦豆子内生真菌对宿主培养物生长及喹诺里西啶类生物碱合成的影响[J].干旱地区农业研究,2017,35(3):212~218 |
| 苦豆子内生真菌对宿主培养物生长及喹诺里西啶类生物碱合成的影响 |
| Effect of Sophora alopecuroides L. endophytic fungi on the growth of the host plant culture and synthesis of quinolizidine alkaloids |
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| DOI：10.7606/j.issn.1000-7601.2017.03.33 |
| 中文关键词: 苦豆子 内生真菌 真菌诱导子 植物培养物 喹诺里西啶生物碱 |
| 英文关键词:Sophora alopecuroides L. endophytic fungal fungal elicitor plant culture quinolizidine alkaloids |
| 基金项目:国家自然基金项目“苦豆子内生真菌促进宿主喹诺里西啶生物碱合成积累的机制研究”(31260452) |
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| 中文摘要: |
| 利用苦豆子健康植株中分离鉴定的8株内生真菌菌株为真菌诱导子，分别制备灭活菌丝和菌液浓缩物，研究内生真菌诱导子不同种类、浓度和诱导时间对苦豆子无菌苗和愈伤组织的生长以及喹诺里西啶生物碱含量的影响。结果表明：8株苦豆子内生真菌诱导子中，菌液浓缩物的诱导效果要强于灭活菌丝。菌株HMGKDF1菌液浓缩物和灭活菌丝都能明显促进愈伤组织的生长，净生长率是对照的1.82和1.42倍；菌株NDZKDF13菌液浓缩物对愈伤组织生物碱的合成效果明显，生物碱含量为0.5483 mg·g-1，是对照的23.8倍；在一定浓度范围内(0.01～1.0 mg·L-1)，苦豆子内生真菌诱导子能够促进宿主植物喹诺里西啶生物碱的合成。内生真菌诱导子处理苦豆子无菌苗12 d时，喹诺里西啶生物碱含量最高，是对照的2.65倍。在苦豆子无菌苗或愈伤组织中添加一定量的苦豆子内生真菌诱导子，对宿主的生长以及提高喹诺里西啶生物碱含量是一种有效的方法。 |
| 英文摘要: |
| Eight strains of endophytic fungi were isolated and identified as the fungal elicitors from the healthy plant of S. alopecuroides L. and then their autoclaved mycelia and concentrated filtrates were made respectively to study the effect of different types, concentration and eliciting time of endophytic fungal elicitors on the growth of aseptic seedling and callus tissue of S. alopecuroides L., and the content of quinolizidine alkaloids. Among the 8 strains of endophytic fungal elicitors, the elicitation effect of the concentrated filtrate was stronger than those of the autoclaved mycelia. The autoclaved mycelia and concentrated filtrates of HMGKDF1 promoted the growth of callus, with the rate of growth being 1.82 and 1.42 times higher than that in CK, respectively. The concentrated filtrate of NDZKDF13 has a strong effect on the accumulation of quinolizidine alkaloids of S. alopecuroides L. The content of total alkaloids was 0.5483 mg·g-1, 23.8 times higher than that in CK. Within a certain range (0.01～1.0 mg·L-1), endophytic fungal elicitors promoted the synthetic accumulation of quinolizidine alkaloids of the host plant. After 12 days of induction by endophytic fungal elicitors, the content of quinolizidine alkaloids reached the highest amount, 2.65 times higher than that in CK. Addition of the autoclaved mycelia or concentrated filtrates from the endophytic fungi into aseptic seedlings and callus tissues of S. alopecuroides L. could be an effective way to increase the content of quinolizidine alkaloids and to improve the growth of the plant. |
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