[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Editorial Board
Aims& Scopes
Journal News
Articles archive::
All Issues
Current Issue
For Authors::
Call for Papers
Submission Instruction
For Reviewers::
Reviewers Section
Subscription::
Registration Information
Subscription Form
Contact us::
Contact Information
Contact us
Site Facilities::
Site map
Search contents
FAQ
Top 10 contents
Inform to friends
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Journal Citation Index

 

Citation Indices from GS

AllSince 2019
Citations90715466
h-index3825
i10-index237139

 
..
Central Library of Kurdistan University of Medical Sciences
AWT IMAGE
..
Vice-Chancellery for Research and Technology
AWT IMAGE
..
SCImago Journal & Country Rank
:: Volume 28, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2023) ::
SJKU 2023, 28(1): 65-73 Back to browse issues page
Comparison of BrucellacapT Sensitivity and Specificity with Standard Wright Tube Agglutination Test in The Diagnosis of Brucella
Hamid Reza Ghasemi Basir1 , Mohammad Mahdi Majzoobi2 , Abbas Moradi3 , Sadi Hesami Novin4 , Ali Saadatmand 5
1- Associate Professor, Department of Pathology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2- Associate Professor, Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
3- MSc in Epidemiology, Department of community medicine, school of medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- General Practitioner, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
5- MSc in Microbiology, Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , Ali.s_umsha@yahoo.com
Abstract:   (917 Views)
Background and Aim: Brucellosis is one of the most common infectious diseases between humans and livestock in Iran. Because of the diagnostic problems of brucellosis, especially in endemic areas, the use of new diagnostic methods is of great importance. Therefore, this study was performed to compare BrucellacapT sensitivity and specificity with the standard Wright tube agglutination test in the diagnosis of Brucella.
Materials and Methods: A total of 100 sera from a patient with suspected symptoms of brucellosis and without laboratory confirmation were studied. Wright, Coombs wright, and BrucellacapT tests were performed at diagnostic threshold titers of 1.160 and 1.80 Sensitivity, specificity, and positive and negative predictive values were evaluated and finally, data were analyzed with SPSS software version 14.
Results: A total of 100 patients were included in the study, of which 45% were female and 55% were male. In titrate 1.160 positive Wright test as a diagnostic threshold, sensitivity, specificity, and positive and negative predictive values for the BrucellacapT test were 97.95, 86.27, 87.27, and 97.97 percent, respectively. In titrate 1.80 positive Wright test as a diagnostic threshold, sensitivity, specificity, and positive and negative predictive values of BrucellacapT test were 98.43, 97.50, 95.45, and 97.05 percent, respectively.
Conclusion: The results showed that the BrucellacapT test in the studied cut points has acceptable sensitivity, specificity, and positive and negative predictive value for the diagnosis of brucellosis and the diagnostic and accuracy of this test in the titrate 1.160 is very close to the Coombs Wright test.

 
Keywords: Brucellosis, Wright, Coombs Wright, BrucellacapT
Full-Text [PDF 532 kb]   (247 Downloads)    
Type of Study: Original Research | Subject: Microbiology
Received: 2022/01/12 | Accepted: 2022/04/10 | Published: 2023/03/15
References
1. Majzoobi MM, Hashemi SH, Mamani M, Keramat F, Poorolajal J, Ghasemi Basir HR. Effect of hydroxychloroquine on treatment and recurrence of acute brucellosis: a single-blind, randomized clinical trial. Int J Antimicrob Agents. 2018 Mar;51(3):365-369. [DOI:10.1016/j.ijantimicag.2017.08.009] [PMID]
2. Alavi SM, Alavi L. Treatment of brucellosis: a systematic review of studies in recent twenty years. Caspian J Intern Med. 2013 Spring;4(2):636-41.
3. Khalilian MS, Ramazanpour J, Hosseini SM, Narrei S, Zeinalian M. Trends of human brucellosis in Central Iran (2010-2018). J Res Med Sci. 2021 Aug 30;26:55. [DOI:10.4103/jrms.JRMS_621_20] [PMID] []
4. Golshani M, Buozari S. A review of Brucellosis in Iran: Epidemiology, Risk Factors, Diagnosis, Control, and Prevention. Iran Biomed J. 2017 Nov;21(6):349-59.
5. Olsen SC, Palmer MV. Advancement of knowledge of Brucella over the past 50 years. Vet Pathol. 2014 Nov;51(6):1076-89. [DOI:10.1177/0300985814540545] [PMID]
6. Campbell JI, Lan NPH, Phuong PM, Chau LB, Trung Pham Duc, Guzmán-Verri C, Ruiz-Villalobos N, Minh TPT, Muñoz Álvaro PM, Moreno E, Thwaites GE, Rabaa MA, Chau NVV, Baker S. Human Brucella melitensis infections in southern Vietnam. Clin Microbiol Infect. 2017 Nov;23(11):788-790. [DOI:10.1016/j.cmi.2017.06.028] [PMID]
7. Keramat F, Alikhani MY, Poorolajal J, Akbari S. Comparison of serum level of 25(OH) vitamin D3 in brucellosis patients with healthy persons in Hamadan, west of Iran. J Infect Dev Ctries. 2018 Jun 30;12(6):448-453. [DOI:10.3855/jidc.9731] [PMID]
8. Dean AS, Crump L, Greter H, Schelling E, Zinsstag J. Global burden of human brucellosis: a systematic review of disease frequency. PLoS Negl Trop Dis. 2012;6(10):e1865. [DOI:10.1371/journal.pntd.0001865] [PMID] []
9. Lai S, Chen Q, Li Z. Human Brucellosis: An Ongoing Global Health Challenge. China CDC Wkly. 2021 Feb 5;3(6):120-123.
10. Keramat F, Ranjbar M, Mamani M, Hashemi SH, Zeraati F. A comparative trial of three therapeutic regimens: ciprofloxacin-rifampin, ciprofloxacin-doxycycline and doxycycline-rifampin in the treatment of brucellosis. Trop Doct. 2009 Oct;39(4):207-10. [DOI:10.1258/td.2009.090030] [PMID]
11. Mamani M, Majzoobi MM, Keramat F, Varmaghani N, Moghimbeigi A. Seroprevalence of Brucellosis in Butchers, Veterinarians and Slaughterhouse Workers in Hamadan, Western Iran. J Res Health Sci. 2018 Feb 10;18(1):e00406.
12. Vrioni G, Gartzonika C, Kostoula A, Boboyianni C, Papadopoulou C, Levidiotou S. Application of a polymerase chain reaction enzyme immunoassay in peripheral whole blood and serum specimens for diagnosis of acute human brucellosis. Eur J Clin Microbiol Infect Dis. 2004 Mar;23(3):194-9. [DOI:10.1007/s10096-003-1082-4] [PMID]
13. Šimundić AM. Measures of Diagnostic Accuracy: Basic Definitions. EJIFCC. 2009 Jan 20;19(4):203-11.
14. Altman DG, Bland JM. Diagnostic tests. 1: Sensitivity and specificity. BMJ. 1994 Jun 11;308(6943):1552. [DOI:10.1136/bmj.308.6943.1552] [PMID] []
15. Davidson M. The interpretation of diagnostic test: a primer for physiotherapists. Aust J Physiother. 2002;48(3):227-32. [DOI:10.1016/S0004-9514(14)60228-2] [PMID]
16. Peeridogaheh H, Golmohammadi MG, Pourfarzi F. Evaluation of ELISA and Brucellacapt tests for diagnosis of human Brucellosis. Iran J Microbiol. 2013 Mar;5(1):14-8.
17. Yagupsky P, Morata P, Colmenero JD. Laboratory Diagnosis of Human Brucellosis. Clin Microbiol Rev. 2019 Nov 13;33(1):e00073-19. [DOI:10.1128/CMR.00073-19] [PMID] []
18. Yagupsky P. Detection of Brucella melitensis by BACTEC NR660 blood culture system. J Clin Microbiol. 1994 Aug;32(8):1899-901. [DOI:10.1128/jcm.32.8.1899-1901.1994] [PMID] []
19. Serra J, Velasco J, Godoy P, Mendoza J. Puede sustituir la prueba de Brucellacapt a la prueba de Coombs en el diagnóstico de la brucelosis humana? [Can the Brucellacapt test be substituted for the Coombs test in the diagnosis of human brucellosis?]. Enferm Infecc Microbiol Clin. 2001 May;19(5):202-5. Spanish. [DOI:10.1016/S0213-005X(01)72613-4] [PMID]
20. Casanova A, Ariza J, Rubio M, Masuet C, Díaz R. BrucellaCapt versus classical tests in the serological diagnosis and management of human brucellosis. Clin Vaccine Immunol. 2009 Jun;16(6):844-51. [DOI:10.1128/CVI.00348-08] [PMID] []
21. Casao MA, Navarro E, Solera J. Evaluation of Brucellacapt for the diagnosis of human brucellosis. J Infect. 2004 Aug;49(2):102-8. [DOI:10.1016/j.jinf.2004.03.004] [PMID]
22. Memish Z, Mah MW, Al Mahmoud S, Al Shaalan M, Khan MY. Brucella bacteraemia: clinical and laboratory observations in 160 patients. J Infect. 2000 Jan;40(1):59-63. [DOI:10.1053/jinf.1999.0586] [PMID]
23. Dutto L, Pomero F, Allione A. Multiple abscesses in brucellosis with Wright's test negativity. BMJ Case Rep. 2009;2009:bcr06.2008.0243. [DOI:10.1136/bcr.06.2008.0243] [PMID] []
24. Orduña A, Almaraz A, Prado A, Gutierrez MP, Garcia-Pascual A, Dueñas A, et al. Evaluation of an immunocapture-agglutination test (Brucellacapt) for serodiagnosis of human brucellosis. J Clin Microbiol. 2000 Nov;38(11):4000-5. [DOI:10.1128/JCM.38.11.4000-4005.2000] [PMID] []
25. Gómez MC, Nieto JA, Rosa C, Geijo P, Escribano MA, Muñoz A, et al. Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic. Clin Vaccine Immunol. 2008 Jun;15(6):1031-3. [DOI:10.1128/CVI.00424-07] [PMID] []
26. Alişkan H, Colakoğlu S, Turunç T, Demiroğlu YZ, Yazic AC, Arslan H. Brusellozun tanisinda Brucellacapt testinin değerinin araţirilmasi [Evaluation of diagnostic value of Brucellacapt test in brucellosis]. Mikrobiyol Bul. 2007 Oct;41(4):591-5.
27. Majzoobi MM, Hashemi SH, Mamani M, Keramat F, Poorolajal J, Ghasemi Basir HR. Effect of hydroxychloroquine on treatment and recurrence of acute brucellosis: a single-blind, randomized clinical trial. Int J Antimicrob Agents. 2018 Mar;51(3):365-369. [DOI:10.1016/j.ijantimicag.2017.08.009] [PMID]
28. Alavi SM, Alavi L. Treatment of brucellosis: a systematic review of studies in recent twenty years. Caspian J Intern Med. 2013 Spring;4(2):636-41.
29. Khalilian MS, Ramazanpour J, Hosseini SM, Narrei S, Zeinalian M. Trends of human brucellosis in Central Iran (2010-2018). J Res Med Sci. 2021 Aug 30;26:55. [DOI:10.4103/jrms.JRMS_621_20] [PMID] []
30. Golshani M, Buozari S. A review of Brucellosis in Iran: Epidemiology, Risk Factors, Diagnosis, Control, and Prevention. Iran Biomed J. 2017 Nov;21(6):349-59.
31. Olsen SC, Palmer MV. Advancement of knowledge of Brucella over the past 50 years. Vet Pathol. 2014 Nov;51(6):1076-89. [DOI:10.1177/0300985814540545] [PMID]
32. Campbell JI, Lan NPH, Phuong PM, Chau LB, Trung Pham Duc, Guzmán-Verri C, Ruiz-Villalobos N, Minh TPT, Muñoz Álvaro PM, Moreno E, Thwaites GE, Rabaa MA, Chau NVV, Baker S. Human Brucella melitensis infections in southern Vietnam. Clin Microbiol Infect. 2017 Nov;23(11):788-790. [DOI:10.1016/j.cmi.2017.06.028] [PMID]
33. Keramat F, Alikhani MY, Poorolajal J, Akbari S. Comparison of serum level of 25(OH) vitamin D3 in brucellosis patients with healthy persons in Hamadan, west of Iran. J Infect Dev Ctries. 2018 Jun 30;12(6):448-453. [DOI:10.3855/jidc.9731] [PMID]
34. Dean AS, Crump L, Greter H, Schelling E, Zinsstag J. Global burden of human brucellosis: a systematic review of disease frequency. PLoS Negl Trop Dis. 2012;6(10):e1865. [DOI:10.1371/journal.pntd.0001865] [PMID] []
35. Lai S, Chen Q, Li Z. Human Brucellosis: An Ongoing Global Health Challenge. China CDC Wkly. 2021 Feb 5;3(6):120-123.
36. Keramat F, Ranjbar M, Mamani M, Hashemi SH, Zeraati F. A comparative trial of three therapeutic regimens: ciprofloxacin-rifampin, ciprofloxacin-doxycycline and doxycycline-rifampin in the treatment of brucellosis. Trop Doct. 2009 Oct;39(4):207-10. [DOI:10.1258/td.2009.090030] [PMID]
37. Mamani M, Majzoobi MM, Keramat F, Varmaghani N, Moghimbeigi A. Seroprevalence of Brucellosis in Butchers, Veterinarians and Slaughterhouse Workers in Hamadan, Western Iran. J Res Health Sci. 2018 Feb 10;18(1):e00406.
38. Vrioni G, Gartzonika C, Kostoula A, Boboyianni C, Papadopoulou C, Levidiotou S. Application of a polymerase chain reaction enzyme immunoassay in peripheral whole blood and serum specimens for diagnosis of acute human brucellosis. Eur J Clin Microbiol Infect Dis. 2004 Mar;23(3):194-9. [DOI:10.1007/s10096-003-1082-4] [PMID]
39. Šimundić AM. Measures of Diagnostic Accuracy: Basic Definitions. EJIFCC. 2009 Jan 20;19(4):203-11.
40. Altman DG, Bland JM. Diagnostic tests. 1: Sensitivity and specificity. BMJ. 1994 Jun 11;308(6943):1552. [DOI:10.1136/bmj.308.6943.1552] [PMID] []
41. Davidson M. The interpretation of diagnostic test: a primer for physiotherapists. Aust J Physiother. 2002;48(3):227-32. [DOI:10.1016/S0004-9514(14)60228-2] [PMID]
42. Peeridogaheh H, Golmohammadi MG, Pourfarzi F. Evaluation of ELISA and Brucellacapt tests for diagnosis of human Brucellosis. Iran J Microbiol. 2013 Mar;5(1):14-8.
43. Yagupsky P, Morata P, Colmenero JD. Laboratory Diagnosis of Human Brucellosis. Clin Microbiol Rev. 2019 Nov 13;33(1):e00073-19. [DOI:10.1128/CMR.00073-19] [PMID] []
44. Yagupsky P. Detection of Brucella melitensis by BACTEC NR660 blood culture system. J Clin Microbiol. 1994 Aug;32(8):1899-901. [DOI:10.1128/jcm.32.8.1899-1901.1994] [PMID] []
45. Serra J, Velasco J, Godoy P, Mendoza J. Puede sustituir la prueba de Brucellacapt a la prueba de Coombs en el diagnóstico de la brucelosis humana? [Can the Brucellacapt test be substituted for the Coombs test in the diagnosis of human brucellosis?]. Enferm Infecc Microbiol Clin. 2001 May;19(5):202-5. Spanish. [DOI:10.1016/S0213-005X(01)72613-4] [PMID]
46. Casanova A, Ariza J, Rubio M, Masuet C, Díaz R. BrucellaCapt versus classical tests in the serological diagnosis and management of human brucellosis. Clin Vaccine Immunol. 2009 Jun;16(6):844-51. [DOI:10.1128/CVI.00348-08] [PMID] []
47. Casao MA, Navarro E, Solera J. Evaluation of Brucellacapt for the diagnosis of human brucellosis. J Infect. 2004 Aug;49(2):102-8. [DOI:10.1016/j.jinf.2004.03.004] [PMID]
48. Memish Z, Mah MW, Al Mahmoud S, Al Shaalan M, Khan MY. Brucella bacteraemia: clinical and laboratory observations in 160 patients. J Infect. 2000 Jan;40(1):59-63. [DOI:10.1053/jinf.1999.0586] [PMID]
49. Dutto L, Pomero F, Allione A. Multiple abscesses in brucellosis with Wright's test negativity. BMJ Case Rep. 2009;2009:bcr06.2008.0243. [DOI:10.1136/bcr.06.2008.0243] [PMID] []
50. Orduña A, Almaraz A, Prado A, Gutierrez MP, Garcia-Pascual A, Dueñas A, et al. Evaluation of an immunocapture-agglutination test (Brucellacapt) for serodiagnosis of human brucellosis. J Clin Microbiol. 2000 Nov;38(11):4000-5. [DOI:10.1128/JCM.38.11.4000-4005.2000] [PMID] []
51. Gómez MC, Nieto JA, Rosa C, Geijo P, Escribano MA, Muñoz A, et al. Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic. Clin Vaccine Immunol. 2008 Jun;15(6):1031-3. [DOI:10.1128/CVI.00424-07] [PMID] []
52. Alişkan H, Colakoğlu S, Turunç T, Demiroğlu YZ, Yazic AC, Arslan H. Brusellozun tanisinda Brucellacapt testinin değerinin araţirilmasi [Evaluation of diagnostic value of Brucellacapt test in brucellosis]. Mikrobiyol Bul. 2007 Oct;41(4):591-5.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.UMSHA.REC.1399.174

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ghasemi Basir H R, Majzoobi M M, Moradi A, Hesami Novin S, Saadatmand A. Comparison of BrucellacapT Sensitivity and Specificity with Standard Wright Tube Agglutination Test in The Diagnosis of Brucella. SJKU 2023; 28 (1) :65-73
URL: http://sjku.muk.ac.ir/article-1-7180-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 1 (Scientific Journal of Kurdistan University of Medical Sciences 2023) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
مجله علمی دانشگاه علوم پزشکی کردستان Scientific Journal of Kurdistan University of Medical Sciences
Persian site map - English site map - Created in 0.05 seconds with 45 queries by YEKTAWEB 4645