The drastic changes of interior air-temperature and humidity induced by intense heat-mass exchange between inside and outside make the quality of perishable food difficult to guarantee while the door of the refrigerated truck is opened in the process of refrigerated transport. Aiming above problems, a mathematical model of interior temperature of refrigerator truck under the open-door state was established based on the theory of dynamic heat balance, and testified correspondingly through the analysis of influence of parameters on temperature. The test results showed that, when the refrigeration system turned on or off, and the door was opened in the 6min, and the initial temperature of the compartment was 0℃, the former temperature was 0.3℃ lower than the latter temperature. Similarly the former temperature was 0.7℃ lower than the latter temperature, when the refrigeration system turned on or off, and the door was opened in the 6 min, and the initial temperature of the compartment was -15℃. The former temperature was all 0.2℃ lower than the latter temperature, when comparing the speed of the evaporator outlet velocity was 4m/s and 2m /s, or the thermal conductivity of insulating materials was 0.05W/(m·K) and 0.033W/(m·K), and the door was opened in the 6min, and the initial temperature of the compartment was 0℃. Besides, the air-temperature increased more correspondingly inside refrigerated compartment in the situation of big temperature difference between interior and exterior and wider door-opening. Lastly, the interior heating rate was different according to the wind and wind direction outside of refrigerator carriage with the door opened. 