The antimicrobial effects of the volatile organic components (VOCs) from Streptomyces alboflavus TD-1 on Botrytis cinerea were tested by two-sealed-base-plates method and illustrated by scanning electron micrographs (SEM). Furthermore, the chemical component of VOCs was analyzed by GC-MS in this study. The results showed that VOCs from S. alboflavus TD-1 grown on autoclaved wheat bran had a certain degree of antifungi activity on the growth of Botrytis cinerea, while the inhibitory ratio reached the strongest with 66.67% after 5 days. The sporulation was significantly inhibited (p<0.01), and morphological abnormalities, rough surface as well as increased cell membrane permeability were observed after 5 days. Additionally, the main chemical components of VOCs were 2-MIB (13.52%) and 1,4-dimethyl adamantine (14.08%). All these results suggest that the VOCs from S. alboflavus TD-1 could be developed into a bio-fumigant.