A fast method was developed for determination of 52pesticides in grape wine by using DSPE and GC-MS. The samples were first extracted using acetonitrile with the addition of vibration and ultrasound. Then mix adsorbents were used in dispersive solid phase extraction to purify the extracts. After the high speed centrifugation, nitrogen flushing concentration, and filtration, the samples were detected by GC/MS using selective ion monitoring (SIM). Compared with traditional methods, QuEChERS had advantages in cost and pretreatment speed. The spiked recoveries at three levels of 52pesticides were in range of 60%-110% while the relative standard deviations (n=5) were all less 15%. Meanwhile, the limit of quantity (LOQ) were in the range of 0.0007-0.0323mg/kg. With features such as accuracy and easy operation, this method could be used to fast determination of 52pesticides in grape wine without any special apparatus and also satisfied the requirement of the pesticide routine analysis.