COMPARATIVE ANALYSIS OF THE VASCULAR RESPONSE OF SYNOVIAL AND BONE TISSUE OF THE KNEE JOINT OF RATS IN EXPERIMENTAL NON-SPECIFIC ARTHRITIS AND IN THE INTRODUCTION OF CRYOCONSERVATED PLACENTA

Pelypenko O. V.

MORPHOLOGY

Scentific article

Abstract. Introduction. One of the modern hypotheses of the pathogenesis of osteoarthritis directly relates to circulatory disorders in joint-forming tissues with the subsequent development of dystrophic-degenerative changes. Due to the absence of vessels in cartilage tissue, the study of hemocirculatory processes in the subchondral bone and synovial membrane in normal and in conditions of pathological processes becomes especially relevant. The aim of the research. Comparative assessment of the state of the subchondral bone and synovial membrane microcirculation in the knee joints of rats in normal, in the conditions of experimental arthritis and after exposure to cryopreserved placenta. Material and methods. The experimental research was performed on 115 white rats. 3 control groups of 35 animals in each group were compared with the intact group (10 animals), which performed: 1) modeling of carginan-induced aseptic inflammation of the knee joint; 2) subcutaneous implantation of a fragment of the placenta; 3) subcutaneous implantation of a fragment of the placenta on the background of aseptic inflammation of the knee joint. Histological specimens were prepared according to general methods with hematoxylin-eosin and Van Gizon staining. Results. Filling of vessels of bone and, especially, synovial fabrics was characteristic of the first three days of experiment in all groups of research. The reaction of the microcirculatory bed in the group where aseptic inflammation was simulated differed in the change of this state by the stage of tissue ischemia. In the group of animals injected with cryopreserved placenta, on the contrary, for a long time there was the presence of sludge-phenomenon that indicated the physiology and reversibility of the process. The injection of cryopreserved placenta on the background of aseptic inflammation leveled ischemic phenomena, intensified the processes of angio- and osteogenesis. Conclusions. The microcirculatory system of the synovial and skeletal components of the joint is synchronized in response to the influence of pathological factors. The synovial membrane, as more sensitive, is previously involved in metabolic transformations. The bone component is relatively stable – it later undergoes destructive changes and is reorganized earlier. The injection of cryopreserved placenta can significantly reduce the negative impact of the inflammatory process in experimental osteoarthritis.

osteoarthritis, cryopreserved placenta, microcirculation.

1. Alekseeva LI, Taskina EA, Kashevarova NG. Osteoartrit: epidemiologiya, klassifikatsiya, factory riska i progressirovaniya, klinika, diagnostika, lecheniye. Sovremennaya revmatologiya. 2019;13(2):9-21. [in Russian].
2. Gayko GV, Haluzynskyi OA, Nyzalov TV, Kozak RA, Zaets VB, Cherniak PS. Vyznachennia zalezhnosti formy prohresuvannia koksartrozu vid variant vertykalnoi postavy khvorykh iz kulshovo-poperekovym syndromom. Visnyk ortopedii, travmatolohii ta protezuvannia. 2020;1:48-54. [in Ukrainian].
3. Felson DT, McLaughlin S, Goggins J, LaValley MP, Gale ME, Totterman S, et al. Bone marrow edema and its relation to progression of knee osteoarthritis. Ann Intern Med. 2003 Sep 2;139(5.1):330-6.
4. Kabalyk MA. Rol sosudistykh faktorov v patogeneze osteoartrita. Sovremennyye problemy nauki i obrazovaniya. 2017;2:50. [in Russian].
5. Yakovenchuk NN, Dedukh NV. Morfolohiia suhlobovoho khriashcha ta subkhondralnoi kistky pislia modeliuvannia osteoporozu. Visnyk problem biolohii i medytsyny. 2017;4.3(141):324-7. [in Ukrainian].
6. Chen M, Li Y, Huang X, Gu Y, Li S, Yin P, et al. Skeleton-vasculature chain reaction: a novel insight into the mystery of homeostasis. Bone Res. 2021;9:21. DOI: https://doi.org/10.1038/s41413-021-00138-0.
7. Ramasamy SK, Kusumbe AP, Schiller M, Zeuschner D, Bixel MG, Milia C, et al. Blood flow controls bone vascular function and osteogenesis. Nat Commun. 2016;7:13601. DOI: https://doi.org/10.1038/ncomms13601.
8. Elkerton JS, Xu Y, Pickering JG, Ward AD. Differentiation of arterioles from venules in mouse histology images using machine learning. J Med Imag. 2017;4(2):021104. DOI: 10.1117/1.JMI.4.2.021104.
9. Plakhov AI, Kolesnikova LI, Korytov LI, Vinogradov VG, Darenskaya MA. Izmeneniya pokazateley mikrotsirkulyatsii v ranniy posleoperatsionnyy period pri lechenii diafizarnykh perelomov kostey goleni s pomoshchyu plastiny s ogranichennym kontaktom. Acta Biomedica Scientifica. 2019;4(3):58-62. [in Russian].
10. Grüneboom A, Hawwari I, Weidner D, Culemann S, Müller S, Henneberg S, et al. A network of trans-cortical capillaries as mainstay for blood circulation in long bones. Nat Metab. 2019;1:236-50.
11. Zhao Y, Xie L. Unique bone marrow blood vessels couple angiogenesis and osteogenesis in bone homeostasis and diseases. Ann N Y Acad Sci. 2020;1474(1):5-14.
12. Shepitko VI, Pelypenko AV. Suchasni pohliady na strukturnu orhanizatsiiu suhlobovykh tkanyn. Svit medytsyny ta biolohii. 2015;3(52):175- 83. [in Ukrainian].
13. Bailey KN, Furman BD, Zeitlin J, Kimmerling KA, Wu CL, Guilak F, et al. Intra-articular depletion of macrophages increases acute synovitis and alters macrophage polarity in the injured mouse knee. Osteoarthritis and cartilage. 2020;28(5):626-638.
14. Vagapova VSh, Rybalko DYu, Gumerov AA, Minigazimov RS, Borzilova OKh, Imanova VR. Regeneratsiya sinovialnoy membrany kolennogo sustava posle chastichnoy kapsulektomii v usloviyakh alloplastiki vistseralnoy plevroy v eksperimente. Prakticheskaya meditsina. 2019;1(17):103-7. [in Russian].
15. Pelypenko AV, Shepitko VI, Pelypenko LB. Morfofunktsionalna kharakterystyka mikrotsyrkuliatornoho rusla subkhondralnoi kistky pry vvedenni kriokonservovanoi platsenty na tli aseptychnoho zapalennia suhlobu u shchuriv. Svit medytsyny ta biolohii. 2019;2(68):187-191. [in Ukrainian].
16. Tkachuk PV, Strafun SS, Savosko SI, Makarenko OM. Strukturni porushennia kolinnoho suhlobu pry modeliuvani osteoartrozu. Travma. 2019;20(1):73-8. [in Ukrainian].
17. Komarova EB, Kurinnyi AB. Morfostruktura synovyalnoi obolochky pry revmatoydnom artryte. Morfolohiia. 2013;7(3):65-9. [in Russian].
18. Buch MH, Eyre S, McGonagle D. Persistent inflammatory and non-inflammatory mechanisms in refractory rheumatoid arthritis. Nat Rev Rheumatol. 2021;17(1):17-33.

«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 227-230 pages, index UDK 616.72-002.77.599.323.4

10.29254/2077-4214-2021-2-160-227-230