Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/115074
Title: | Enabling cryo-EM density interpretation from yeast native cell extracts by proteomics data and AlphaFold structures |
Author(s): | Tüting, Christian Schmidt, Lisa Skalidis, Ioannis Sinz, Andrea Kastritis, Panagiotis L. |
Issue Date: | 2023 |
Type: | Article |
Language: | English |
Abstract: | Inthecellularcontext,proteinsparticipateincommunitiestoperformtheirfunction.Thedetectionandidentificationofthesecommunitiesaswellasin-communityinter-actionshaslongbeenthesubjectofinvestigation,mainlythroughproteomicsanalysiswithmassspectrometry.Withtheadventofcryogenicelectronmicroscopyandthe“resolutionrevolution,”theirvisualizationhasrecentlybeenmadepossible,evenincomplex, native samples. The advances in both fields have resulted in the genera-tionoflargeamountsofdata,whoseanalysisrequiresadvancedcomputation,oftenemployingmachinelearningapproachestoreachthedesiredoutcome.Inthiswork,wefirstperformedarobustproteomicsanalysisofmassspectrometry(MS)dataderivedfromayeastnativecellextractandusedthisinformationtoidentifyproteincommu-nitiesandinter-proteininteractions.Cryo-EManalysisofthecellextractprovidedareconstructionofabiomoleculeatmediumresolution(∼8Å(FSC=0.143)).Utiliz-ingMS-derivedproteomicsdataandsystematicfittingofAlphaFold-predictedatomicmodels, this density was assigned to the 2.6 MDa complex of yeast fatty acid syn-thase.OurproposedworkflowidentifiesproteincomplexesinnativecellextractsfromSaccharomyces cerevisiaeby combining proteomics, cryo-EM, and AI-guided proteinstructureprediction. |
URI: | https://opendata.uni-halle.de//handle/1981185920/117030 http://dx.doi.org/10.25673/115074 |
Open Access: | Open access publication |
License: | (CC BY 4.0) Creative Commons Attribution 4.0 |
Journal Title: | Proteomics |
Publisher: | Wiley VCH |
Publisher Place: | Weinheim |
Volume: | 23 |
Issue: | 17 |
Original Publication: | 10.1002/pmic.202200096 |
Page Start: | 1 |
Page End: | 10 |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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Proteomics - 2023 - Tüting - Enabling cryo‐EM density interpretation from yeast native cell extracts by proteomics data and.pdf | 1.96 MB | Adobe PDF | View/Open |