The W-Ni alloy specimens were prepared by laser powder bed fusion (LPBF) using 93W-7Ni pre-mixed powder feedstock. The influence of different linear energy densities (LEDs) on relative density, microstructure and microhardness of LPBFed W-Ni alloy specimens. The results show that the defects of the W-Ni alloy specimens are mainly irregular pores with no obvious cracks and the irregular pores can be reduced by increasing the LED. The W-Ni alloy specimens with a maximum relative density of 98.04% can be prepared by using LED of 1.5J/mm. The microstructure of LPBFed W-Ni alloy specimens is mainly composed of W particles and Ni-W matrix phase, accompanied by a large number of fine dendritic/granular W grains. The LPBFed W-Ni specimens have high microhardness with a maximum of 529.83HV0.5, which was not significantly influenced by LED.