结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展

赵泽鑫; 周灵; 韩美玲; 许源; 蔡俊

doi:10.12187/2024.01.001

结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展

湖北工业大学生物工程与食品学院/发酵工程教育部重点实验室/工业发酵湖北省协同创新中心/工业微生物湖北省重点实验室, 湖北武汉 430068

作者简介: 赵泽鑫(1991-),男,湖南省郴州市人,湖北工业大学讲师,博士,主要研究方向为酶工程、生物转化。E-mail:zx_zhao@hbut.edu.cn;

基金项目: 湖北省教育厅科技攻关计划青年人才项目(Q20266407)
国家自然科学基金青年基金项目(32302019)
中图分类号: TS221

Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering

School of Bioengineering and Food science/Key Laboratory of Fermentation Engineering/Hubei Collaborative Innovation Center of Industrial Fermentation/Hubei Key Laboratory of Industrial Mincrobiology, Hubei University of Technology, Wuhan 430068, China
Received Date: 2023-08-26
Accepted Date: 2023-10-12

CLC number: TS221

摘要: 基于利用脂肪酶对天然油脂进行改性生成结构脂质(Structured Lipids,SLs)可赋予油脂额外的健康属性,有效提高产品附加值,对近期报道的各类SLs酶法合成及通过酶工程技术(结构设计和固定化)提升合成效果的研究进行综述,认为,使用sn-1,3位置专一性脂肪酶可精准合成SLs,利用分段变温反应、超临界流体反应、真空反应、超声波辅助等途径可有效抑制酰基转移,从而提高产物得率;脂肪酶的结构设计可提高其催化效率、稳定性和底物专一性,使SLs的合成效率和得率有所提升;固定化脂肪酶技术也能提高脂肪酶的反应活性和稳定性,增强其重复利用性,降低SLs酶法合成的成本。未来应针对不同SLs的结构特点,通过结构设计获得高活性、高稳定性和专一性的酶分子,结合新型固定化技术创制高性能酶制剂,以期为高效、精准的SLs酶法合成工艺的创新发展提供参考。
- 结构脂质 /
- 脂肪酶 /
- 酶法合成 /
- 酶工程 /
- 结构设计 /
- 固定化
Abstract: The utilization of lipase in synthesis structured lipids (SLs) from natural oils confers additional health properties and effectively enhances their value-added potential. This paper provided a comprehensive review of recent studies on the enzymatic preparation of SLs, summarizing the advancements in structural design and immobilized enzyme engineering technology to improve the efficiency of their synthesis by enzymatic approaches. The sn-1,3 regiospecific lipase enabled the precise synthesis of SLs, while employing stepwise variable temperature reactions, supercritical fluid reactions, vacuum reactions, and ultrasonic-assisted methods could inhibit the acyl migration effects and further enhance product yield. Furthermore, structural design could enhance the catalytic efficiency, stability, and substrate specificity of lipases to elevate the efficiency and yield in enzymatic synthesis of SLs. Immobilized enzyme technology could enhance enzyme reactivity and stability while reducing the costs of enzymatic synthesis of SLs. In the future, the high-performance enzymes should be developed by combining structural modification and novel immobilization technology on the basis of the clearer understanding of their structure-function characteristics. These insights would contribute to innovation and advancement in efficient and precise enzymatic synthesis processes for SLs.
- structured lipids /
- lipase /
- enzymatic synthesis /
- enzyme engineering /
- structure design /
- immobilization
1. [1]
  ZAM W.Structured lipids:Methods of production, commercial products and nutraceutical characteristics[J].Progress in Nutrition,2015,17(3):198-213.
2. [2]
  李亚,时杰,黄凤洪.酶催化法制备新型结构脂质研究进展[J].生物产业技术,2019(4):42-47.
3. [3]
  中国共产党中央委员会,中华人民共和国国务院.“健康中国2030”规划纲要[EB/OL].(2016-10-25
  )[2023-06-25].https://www.gov.cn/zhengce/2016-10/25/content_5124174.htm.
4. [4]
  GUO Y L,CAI Z X,XIE Y P,et al.Synthesis,physicochemical properties,and health aspects of structured lipids:A review[J].Comprehensive Reviews in Food Science and Food Safety,2020,19(2):759-800.
5. [5]
  ARAB-TEHRANY E,JACQUOT M,GAIANI C,et al.Beneficial effects and oxidative stability of omega-3 long-chain polyunsaturated fatty acids[J].Trends in Food Science & Technology,2012,25(1):24-33.
6. [6]
  KIM J,CHUNG M Y,CHOI H D,et al.Enzymatic synthesis of structured monogalactosyl diacylglycerols enriched in pinolenic acid[J].Journal of Agricultural and Food Chemistry,2018,66(30):8079-8085.
7. [7]
  HE Y J,QIU C Y,GUO Z,et al.Production of new human milk fat substitutes by enzymatic acidolysis of microalgae oils from Nannochloropsis oculata and Isochrysis galbana[J].Bioresource Technology,2017,238:129-138.
8. [8]
  MA G J,DAI L M,LIU D H,et al.Lipase-mediated selective methanolysis of fish oil for biodiesel production and polyunsaturated fatty acid enrichment[J].Energy & Fuels,2018,32(7):7630-7635.
9. [9]
  CAO X,MANGAS-SNCHEZ J,FENG F Q,et al.Acyl migration in enzymatic interesterification of triacylglycerols:Effects of lipases from Thermomyces lanuginosus and Rhizopus oryzae,support material, and water activity[J].European Journal of Lipid Science and Technology,2016,118(10):1579-1587.
10. [10]
  万思迪.疏棉状嗜热丝孢菌脂肪酶热稳定性和催化功能的理性设计研究[D].昆明:云南师范大学,2022.
11. [11]
  KIM B H,AKOH C C.Recent research trends on the enzymatic synthesis of structured lipids[J].Journal of Food Science,2015,80(8):C1713-C1724.
12. [12]
  ZHOU J,LEE Y Y,MAO Y L,et al.Future of structured lipids: Enzymatic synthesis and their new applications in food systems[J].Foods,2022,11(16):2400.
13. [13]
  ROGALSKA E,CUDREY C,FERRATO F,et al.Stereoselective hydrolysis of triglycerides by animal and microbial lipases[J].Chirality,1993,5(1):24-30.
14. [14]
  WEI W,JIN Q Z,WANG X G.Human milk fat substitutes: Past achievements and current trends[J].Progress in Lipid Research,2019,74:69-86.
15. [15]
  LIU Z Q,DAI L M,LIU D H,et al.Progress and perspectives of enzymatic preparation of human milk fat substitutes[J].Biotechnology for Biofuels and Bioproducts,2022,15(1):118.
16. [16]
  HASIBUAN H A.The synthesis of sn-2 palmitate as human milk fat substitute from palm oil fractions by enzymatic interesterification: A review[J].Journal of Oil Palm Research,2022,34(4):608-621.
17. [17]
  高亮,余旭伟,邹凤,等.酶法合成1-油酸-2-棕榈酸-3-亚油酸甘油三酯结构脂的研究[J].中国油脂,2020,45(8):66-70.
18. [18]
  XIONG Z Q,PAN L J,JIANG S T,et al.Optimization of two-step lipase-catalyzed synthesis of human milk fat substitutes by response surface methodology and fatty acid composition analysis[J].Food Science,2017,38(2):248-254.
19. [19]
  AGAPAY R C,JU Y H,TRAN-NGUYEN P L,et al.Process evaluation of solvent-free lipase-catalyzed esterification schemes in the synthesis of structured triglycerides from oleic and palmitic acids[J].Asia-Pacific Journal of Chemical Engineering,2021,16(2):e2606.
20. [20]
  肖乾煌,徐雨茜,熊华,等.溶剂和水分活度对OPO结构脂合成中酰基迁移的影响[J].中国油脂,2020,45(3):62-67.
21. [21]
  熊志琴,潘丽军,姜绍通,等.响应面试验优化两步酶法合成母乳脂替代品工艺及脂肪酸组成分析[J].食品科学,2017,38(2):248-254.
22. [22]
  LI Y, LI C,FENG F Q,et al.Synthesis of medium and long-chain triacylglycerols by enzymatic acidolysis of algal oil and lauric acid[J].LWT-Food Science and Technology,2021,136(1):110309.
23. [23]
  GHIDE M K,LI K,WANG J H,et al.Effective production of human milk fat substitutes rich in 1,3-dioleoyl-2-palmitoyl glycerol (OPO) viaa new strategy[J].Food Biophysics,2022,17(4):495-507.
24. [24]
  TECELO C,PERRIER V,DUBREUCQ E,et al.Production of human milk fat substitutes by interesterification of tripalmitin with ethyl oleate catalyzed by Candida parapsilosis lipase/acyltransferase[J].Journal of the American Oil Chemists Society,2019,96(7):777-787.
25. [25]
  UTAMA Q D,SITANGGANG A B,ADAWIYAH D R,et al.Lipase-catalyzed interesterification for the synthesis of medium-long-medium (MLM) structured lipids:A review[J].Food Technology and Biotechnology,2019,57(3):305-318.
26. [26]
  JENNINGS B H,SHEWFELT R L,AKOH C C.Food applications of a rice bran oil structured lipid in fried sweet potato chips and an energy bar[J].Journal of Food Quality,2010,33(6):679-692.
27. [27]
  王博,鞠兴荣,周润松,等.大豆油和月桂酸制备MLM型结构脂质工艺优化及其性质分析[J].食品科学,2018,39(18):249-254.
28. [28]
  CABALLERO E,SOTO C,OLIVARES A,et al.Potential use of avocado oil on structured lipids MLM-type production catalysed by commercial immobilised lipases[J].PLoS One,2014,9(9):e107749.
29. [29]
  王强,贺稚非,李贵节,等.脂肪酶催化玉米胚芽油与辛酸制备MLM型结构脂质[J].中国食品学报,2017,17(1):77-83.
30. [30]
  JADHAV H B,GOGATE P,ANNAPURE U.Process intensification of acidolysis reaction catalysed by enzymes for synthesis of designer lipids using sonication[J].Chemical Engineering Journal,2022,428:131374.
31. [31]
  MORALES-MEDINA R,MUNIO M,GUADIX A,et al.Development of an up-grading process to produce MLM structured lipids from sardine discards[J].Food Chemistry,2017,228:634-642.
32. [32]
  LEE Y Y,TANG T K,PHUAH E T,et al.Production, safety, health effects and applications of diacylglycerol functional oil in food systems:A review[J].Critical Reviews in Food Science and Nutrition,2019,60(15):2509-2525.
33. [33]
  ANDO Y,SAITO S,YAMANAKA N,et al.Alpha linolenic acid-enriched diacylglycerol consumption enhances dietary fat oxidation in healthy subjects:A randomized double-blind controlled trial[J].Journal of Oleo Science,2017,66(2):181-185.
34. [34]
  DEVI B L A P,GANGADHAR K N,PRASAD R B N,et al.Nutritionally enriched 1,3-diacylglycerol-rich oil:Low calorie fat with hypolipidemic effects in rats[J].Food Chemistry,2018,248:210-216.
35. [35]
  KIM H,CHOE J H,CHOI J H,et al.Medium-chain enriched diacylglycerol (MCE-DAG) oil decreases body fat mass in mice by increasing lipolysis and thermogenesis in adipose tissue[J].Lipids,2017,52(8):665-673.
36. [36]
  杨馨怡.1,3-甘油二酯的酶法制备及其功效评价研究[D].泰安:山东农业大学,2022.
37. [37]
  LEE Y Y,TANG T K,PHUAH E T,et al.Production,safety,health effects and applications of diacylglycerol functional oil in food systems:A review[J].Critical Reviews in Food Science and Nutrition,2020,60(15):2509-2525.
38. [38]
  WANG Z T,DAI L M,LIU D H,et al.Kinetics and mechanism of solvent influence on the lipase-catalyzed 1,3-diolein synthesis[J].ACS Omega,2020,5(38):24708-24716.
39. [39]
  连伟帅.甘油二酯,LML型结构脂的酶法制备与应用研究[D].广州:华南理工大学,2019.
40. [40]
  MENG X,XU G,ZHOU Q L,et al.Highly efficient solvent-free synthesis of 1,3-diacylglycerols by lipase immobilised on nano-sized magnetite particles[J].Food Chemistry,2014,143:319-324.
41. [41]
  YANG T,FRUEKILDE M B,XU X.Suppression of acyl migration in enzymatic production of structured lipids through temperature programming[J].Food Chemistry,2005,92(1):101-107.
42. [42]
  KIM I H,KO S N,LEE S M,et al.Production of structured lipids by lipase-catalyzed acidolysis in supercritical carbon dioxide: Effect on acyl migration[J].Journal of the American Oil Chemists Society,2004,81:537-541.
43. [43]
  ZHONG N J,GUI Z Y,XU L,et al.Solvent-free enzymatic synthesis of 1,3-diacylglycerols by direct esterification of glycerol with saturated fatty acids[J].Lipids in Health and Disease,2013,12:65.
44. [44]
  LIU S L,DONG X Y,WEI F,et al.Ultrasonic pretreatment in lipase-catalyzed synthesis of structured lipids with high 1,3-dioleoyl-2-palmitoylglycerol content[J].Ultrasonics Sonochemistry,2015,23:100-108.
45. [45]
  ZHANG H P,ZHENG M M,SHI J,et al.Enzymatic preparation of “functional oil” rich in feruloylated structured lipids with solvent-free ultrasound pretreatment[J].Food Chemistry,2018,248:272-278.
46. [46]
  ANG X,CHEN H,XIANG J Q,et al.Preparation and functionality of lipase-catalysed structured phospholipid:A review[J].Trends in Food Science & Technology,2019,88:373-383.
47. [47]
  LI Y H,DAI L M,LIU D H,et al.Progress & prospect of enzyme-mediated structured phospholipids preparation[J].Catalysts,2022,12(7):795.
48. [48]
  RYCHLICKA M,NIEZGODA N,GLISZCZYN＇SKA A.Lipase-catalyzed acidolysis of egg-yolk phosphatidylcholine with Citronellic acid.new insight into synthesis of isoprenoid-phospholipids[J].Molecules,2018,23(2):314.
49. [49]
  LIANG S H,LIU Y M,MENG Y N,et al.Two-stage enzymatic hydrolysis of soybean concentrated phospholipid to prepare glycerylphosphorylcholine:Optimized by response surface methodology[J].Journal of Oleo Science,2021,70(2):237-245.
50. [50]
  李珍珍,许飞跃,韩玉谦,等.超临界 CO₂体系中磷脂酶A2催化合成高DHA含量DHA-PC的研究[J].食品工业科技,2017,38(18):85-89.
51. [51]
  肖志刚,杨国强,杨庆余,等.酶法合成亚麻酸磷脂的结构特性及抗氧化性[J].食品科学,2020,41(22):57-63.
52. [52]
  OKULUS M,RYCHLICKA M,GLISZCZYN＇SKA A.Enzymatic production of biologically active 3-methoxycinnamoylated lysophosphatidylcholine via regioselctive lipase-catalyzed acidolysis[J].Foods,2021,11(1):7.
53. [53]
  张芹,孙兆敏,徐杰,等.酶法合成富含二十二碳六烯酸的磷脂酰丝氨酸[J].中国食品学报,2016,16(3):81-87.
54. [54]
  廖妙飞,付万冬,孙继鹏,等.富含ω-3多不饱和脂肪酸的磷脂酰丝氨酸制备工艺研究[J].渔业研究,2022,44(6):599-605.
55. [55]
  HUANG Z X,GUO Z W,XIE D,et al.Rhizomucor miehei lipase-catalysed synthesis of cocoa butter equivalent from palm mid-fraction and stearic acid:Characteristics and feasibility as cocoa butter alternative[J].Food Chemistry,2021,343:128407.
56. [56]
  LIAN W S,WANG W F,TAN C P,et al.Immobilized Talaromyces thermophilus lipase as an efficient catalyst for the production of LML-type structured lipids[J].Bioprocess and Biosystems Engineering,2018,42(2):321-329.
57. [57]
  ZHANG J H,JIANG Y Y,LIN Y,et al.Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids[J].PLoS One,2013,8(7):e67892.
58. [58]
  LI L L,WANG Y H,CUI R G,et al.Engineering the thermostability of the mono-and diacylglycerol lipase SMG1 for the synthesis of diacylglycerols[J].Foods,2022,11(24):4069.
59. [59]
  WOO J M,KANG Y S,LEE S M,et al.Substrate-binding site engineering of Candida antarctica lipase B to improve selectivity for synthesis of 1-monoacyl-sn-glycerols[J].Biotechnology and Bioprocess Engineering,2022,27(2):234-243.
60. [60]
  SCHRAG J D,LI Y,WU S,et al.Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum[J].Nature,1991,351(6329):761-764.
61. [61]
  TANG T,YUAN C L,HWANG H T,et al.Engineering surface hydrophobicity improves activity of Bacillus thermocatenulatus lipase 2 enzyme[J].Biotechnology Journal,2015,10(11):1762-1769.
62. [62]
  赵泽鑫.基于结构信息和生物计算的MAS1和PCL脂肪酶分子改造研究[D].广州:华南理工大学,2020.
63. [63]
  LAN D M,LI S,TANG W,et al.Glycerol is released from a new path in MGL lipase catalytic process[J].Journal of Chemical Information and Modeling,2021,62(9):2248-2256.
64. [64]
  WANG W G,DASETTY S,SARUPRIA S,et al.Rational engineering of low temperature activity in thermoalkalophilic Geobacillus thermocatenulatus lipase[J].Biochemical Engineering Journal,2021,174:108093.
65. [65]
  HAMDAN S H,MAIANGWA J,NEZHAD N G,et al.Knotting terminal ends of mutant T1 lipase with disulfide bond improved structure rigidity and stability[J].Applied Microbiology and Biotechnology,2023,107(5/6):1673-1686.
66. [66]
  HAN Z L,HAN S Y,ZHENG S P,et al.Enhancing thermostability of a Rhizomucor miehei lipase by engineering a disulfide bond and displaying on the yeast cell surface[J].Applied Microbiology and Biotechnology,2009,85:117-126.
67. [67]
  LI L L,WU W K,DENG Z X,et al.Improved thermostability of lipase Lip2 from Yarrowia lipolytica through disulfide bond design for preparation of medium-long-medium structured lipids[J].LWT-Food Science and Technology,2022,166:113786.
68. [68]
  TAMBUNAN U S F,RANDY A,PARIKESIT A A.Design of Candida antarctica lipase B thermostability improvement by introducing extra disulfide bond into the enzyme[J].OnLine Journal of Biological Sciences,2014,14(2):108-118.
69. [69]
  YU X W,TAN N J,XIAO R,et al.Engineering a disulfide bond in the lid hinge region of Rhizopus chinensis lipase:Increased thermostability and altered acyl chain length specificity[J].PLoS One,2012,7(10):e46388.
70. [70]
  ZHANG H T,SANG J C,ZHANG Y,et al.Rational design of a Yarrowia lipolytica derived lipase for improved thermostability[J].International Journal of Biological Macromolecules,2019,137:1190-1198.
71. [71]
  WU J P,LI M,ZHOU Y,et al.Introducing a salt bridge into the lipase of Stenotrophomonas maltophilia results in a very large increase in thermal stability[J].Biotechnology Letters,2015,37(2):403-407.
72. [72]
  GIHAZ S,KANTEEV M,PAZY Y,et al.Filling the void:Introducing aromatic interactions into solvent tunnels to enhance lipase stability in methanol[J].Applied and Environmental Microbiology,2018,84(23):e02143-18.
73. [73]
  BRUNDIEK H B,EVITT A S,KOURIST R,et al.Creation of a lipase highly selective for trans fatty acids by protein engineering[J].Angewandte Chemie International Edition,2012,51(2):412-414.
74. [74]
  YANG J H,KOGA Y C,NAKANO H,et al.Modifying the chain-length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate-binding site[J].Protein Engineering,2002,15(2):147-152.
75. [75]
  YEN C C,MALMIS C C,LEE G C,et al.Site-specific saturation mutagenesis on residues 132 and 450 of Candida rugosa LIP2 enhances catalytic efficiency and alters substrate specificity in various chain lengths of triglycerides and esters[J].Journal of Agricultural and Food Chemistry,2010,58(20):10899-10905.
76. [76]
  BRUNDIEK H,PADHI S K,KOURIST R,et al.Altering the scissile fatty acid binding site of Candida antarctica lipase A by protein engineering for the selective hydrolysis of medium chain fatty acids[J].European Journal of Lipid Science and Technology,2012,114(10):1148-1153.
77. [77]
  曹茜,王丹,袁永俊.脂肪酶位置选择性及其应用在功能性结构甘油三酯合成中的研究进展[J].食品与发酵工业,2020,46(11):295-301.
78. [78]
  ZHAO Z X,CHEN S Y,XU L,et al.Structural basis for the regiospecificity of a lipase from Streptomyces sp.W007[J].International Journal of Molecular Sciences,2022,23(10):5822.
79. [79]
  CAI Y Z,BAI Q Y,YANG L F,et al.Substitution and mutation of the C-terminal loop domain of Penicillium expansum lipase significantly changed its regioselectivity and activity[J].Biochemical Engineering Journal,2022,179:108313.
80. [80]
  JEGANNATHAN K R,ABANG S,PONCELET D,et al.Production of biodiesel using immobilized lipase:A critical review[J].Critical Reviews in Biotechnology,2008,28(4):253-264.
81. [81]
  REMONATTO D,MIOTTI JR R H,MONTI R,et al.Applications of immobilized lipases in enzymatic reactors:A review[J].Process Biochemistry,2022,114:1-20.
82. [82]
  GAO S L,WANG Y J,WANG W W,et al.Enhancing performance of lipase immobilized on methyl-modified silica aerogels at the adsorption and catalysis processes:Effect of cosolvents[J].Journal of Molecular Catalysis B(Enzymatic),2010,62(3/4):218-224.
83. [83]
  ALMEIDA L C,BARBOSA A S,FRICKS A T,et al.Use of conventional or non-conventional treatments of biochar for lipase immobilization[J].Process Biochemistry,2017,61:124-129.
84. [84]
  HOANG HIEP N,AND MOONIL K.An overview of techniques in enzyme immobilization[J].Applied Science and Convergence Technology,2017,26(6):157-163.
85. [85]
  HE L H,ZHENG J W,FENG S T,et al.Immobilization of Candida antarctica lipase A onto macroporous resin NKA-9:Esterification and glycerolysis performance study[J].Journal of Oleo Science,2022,71(9):1337-1348.
86. [86]
  PACHECO B J S,DOMINGUES O,REINA M P,et al.Improved the synthesis of dietary triglycerides by using lipase supported on clay carriers[J].Biotechnology Journal,2022,17(4):2100491.
87. [87]
  WANG X M,ZHAO X X,QIN X L,et al.Properties of immobilized MAS1-H108A lipase and its application in the efficient synthesis of n-3 PUFA-rich triacylglycerols[J].Bioprocess and Biosystems Engineering,2021,44(3):575-584.
88. [88]
  ZHANG B H,WENG Y Q,XU H,et al.Enzyme immobilization for biodiesel production[J].Applied Microbiology and Biotechnology,2012,93(1):61-70.
89. [89]
  WANG W F,XU Y,QIN X L,et al.Immobilization of lipase SMG1 and its application in synthesis of partial glycerides[J].European Journal of Lipid Science and Technology,2014,116(8):1063-1069.
90. [90]
  CAI X Y,ZHANG M J,WEI W,et al.The immobilization of Candida antarctica lipase B by ZIF-8 encapsulation and macroporous resin adsorption:Preparation and characterizations[J].Biotechnology Letters,2020,42(2):269-276.
91. [91]
  ABDULMALEK S A,YAN Y J.Recent developments of lipase immobilization technology and application of immobilized lipase mixtures for biodiesel production[J].Biofuels Bioprod Biorefining,2022,16(4):1062-1094.
92. [92]
  AKIL E,PEREIRA A D,EL-BACHA T,et al.Efficient production of bioactive structured lipids by fast acidolysis catalyzed by Yarrowia lipolytica lipase, free and immobilized in chitosan-alginate beads,in solvent-free medium[J].International Journal of Biological Macromolecules,2020,163:910-918.
93. [93]
  CHEN W Y,XU L,ZHONG N J.Encapsulation of CALB by nucleotide/metal ions coordination nanoparticles:Highly selective catalysis of esterification while poor performance in glycerolysis reaction[J].Journal of the Science of Food and Agriculture,2022,102(5):1812-1822.
94. [94]
  LI M M,QIAO S,ZHENG Y L,et al.Fabricating covalent organic framework capsules with commodious microenvironment for enzymes[J].Journal of the American Chemical Society,2020,142(14):6675-6681.
95. [95]
  CAVALCANTE F T T,CAVALCANTE A L G,DE SOUSA I G,et al.Current status and future perspectives of supports and protocols for enzyme immobilization[J].Catalysts,2021,11(10):1222.
96. [96]
  MOHAMAD N R,MARZUKI N H C,BUANG N A,et al.An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes[J].Biotechnology & Biotechnological Equipment,2015,29(2):205-220.
97. [97]
  ISMAIL A R,BAEK K H.Lipase immobilization with support materials, preparation techniques,and applications:Present and future aspects[J].International Journal of Biological Macromolecules,2020,163:1624-1639.
98. [98]
  LI X X,LI D M,WANG W F,et al.Immobilization of SMG1-F278N lipase onto a novel epoxy resin:Characterization and its application in synthesis of partial glycerides[J].Journal of Molecular Catalysis B(Enzymatic),2016,133:154-160.
99. [99]
  GONCALVES D,SILVA A G,GUIDINI C Z.Lipases:Sources,immobilization methods,and industrial applications[J].Applied Microbiology and Biotechnology,2019,103(18):7399-7423.
100. [100]
  BAVARO T,BENUCCI I,PEDRALI A,et al.Lipase-mediated hydrolysis of hempseed oil in a packed-bed reactor and in-line purification of PUFA as mono-and diacylglycerols[J].Food Bioprod Process,2020,123:345-353.
101. [101]
  ZHAO J F,LIN J P,YANG L R,et al.Enhanced performance of rhizopus oryzae lipase by reasonable immobilization on magnetic nanoparticles and its application in synthesis 1,3-diacyglycerol[J].Applied Biochemistry and Biotechnology,2019,188(3):677-689.
102. [102]
  XIE W L,ZANG X Z.Immobilized lipase on core-shell structured Fe₃O₄-MCM-41 nanocomposites as a magnetically recyclable biocatalyst for interesterification of soybean oil and lard[J].Food Chemistry,2016,194:1283-1292.
1. [1]
  郭华诚 , 胡仙妹 , 高尊华 , 李金周 , 王红霞 . 针辊式烟丝结构调控设备参数变化对细支烟卷制品质的影响. 轻工学报, 2024, 0(0): -.
2. [2]
  郭华诚 , 胡仙妹 , 高尊华 , 李金周 , 王红霞 . 针辊式烟丝结构调控设备参数变化对细支烟卷制品质的影响. 轻工学报, 2024, 39(2): 122-126. doi: 10.12187/2024.02.016
3. [3]
  张义森 , 程硕 , 周娟娟 , 贾会领 , 王军 , 陈雪 , 吴丽芳 . 杜仲叶提取物绿色合成纳米铂及其美白作用. 轻工学报, 2023, 0(0): 56-63.
4. [4]
  罗亮 , 师东方 , 朱鲜艳 , 宗东岳 , 王明辉 , 王鹏飞 , 金强 , 李朝建 . 基于增量式PID算法的香精施加系统设计. 轻工学报, 2024, 0(0): -.
5. [5]
  罗亮 , 师东方 , 朱鲜艳 , 宗东岳 , 王明辉 , 王鹏飞 , 金强 , 李朝建 . 基于增量式PID算法的香精施加系统设计. 轻工学报, 2024, 39(2): 114-121. doi: 10.12187/2024.02.015
6. [6]
  方亦成 , 杨菁 , 陆诚玮 , 冯奇 , 汪旭 . 基于层次分析法的加热卷烟感官评价指标筛选及其权重建立. 轻工学报, 2024, 0(0): -.
7. [7]
  方亦成 , 杨菁 , 陆诚玮 , 冯奇 , 汪旭 . 基于层次分析法的加热卷烟感官评价指标筛选及其权重建立. 轻工学报, 2024, 39(2): 107-113. doi: 10.12187/2024.02.014

点击查看大图

计量

PDF下载量: 29
文章访问数: 1286
引证文献数: 0

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展

作者简介: 赵泽鑫(1991-),男,湖南省郴州市人,湖北工业大学讲师,博士,主要研究方向为酶工程、生物转化。E-mail:zx_zhao@hbut.edu.cn;

Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering

计量

通讯作者: 陈斌, bchen63@163.com

结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展

作者简介:赵泽鑫(1991-),男,湖南省郴州市人,湖北工业大学讲师,博士,主要研究方向为酶工程、生物转化。E-mail:zx_zhao@hbut.edu.cn

English Abstract

Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering

目录

结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展

作者简介: 赵泽鑫(1991-),男,湖南省郴州市人,湖北工业大学讲师,博士,主要研究方向为酶工程、生物转化。E-mail:zx_zhao@hbut.edu.cn;

Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

结构脂质的酶法合成及应用酶工程提高其合成效果的研究进展

作者简介:赵泽鑫(1991-),男,湖南省郴州市人,湖北工业大学讲师,博士,主要研究方向为酶工程、生物转化。E-mail:zx_zhao@hbut.edu.cn

English Abstract

Research progress in enzymatic synthesis of structured lipids and improvement of the synthetic effect by enzyme engineering

目录