外源褪黑素对镉胁迫下小麦幼苗生长生理特征及镉含量的影响
Effects of exogenous melatonin on growth and physiological characteristics and Cd content of wheat seeding under Cd stresses
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摘要: 以小麦矮抗58为研究对象,采用室内培养实验对不同浓度镉(Cd)胁迫下外源褪黑素处理对小麦幼苗生长生理特征及Cd含量进行了研究。结果显示:在Cd胁迫下,外源褪黑素处理在一定程度上促进了小麦幼苗的生长;随着褪黑素浓度的增加,小麦芽中超氧化物歧化酶活性、过氧化氢酶活性和过氧化物酶活性总体上呈先增加后降低的趋势,丙二醛含量则在低浓度Cd胁迫下有所降低、在高浓度Cd胁迫下有所增加;外源褪黑素在一定程度上显著降低了小麦根部和芽中的Cd含量,在低水平Cd胁迫浓度(100 μmol·L-1)时,Cd在小麦中的转移系数显著增加,说明外源褪黑素在影响小麦对Cd吸收的同时促进了Cd在小麦中的转移,但在Cd浓度增加的情况下,较高浓度褪黑素对Cd在小麦幼苗中转移的影响并不显著。Abstract: To investigate the effects of exogenous melatonin on the growth physiology and Cd content of wheat seedling under Cd stress, the present research selected wheat seed Aikang No.58 as test material and adopted indoor culture method to study the effects of different concentrations exogenous melatonin under the stress of different Cd concentrations on the growth, physiological characteristics, and Cd absorption of wheat seedling. Results showed that under Cd stress, the addition of exogenous melatonin promoted the growth of wheat seedling to some extent. With the increase of melatonin concentration, the activities of peroxidase, catalase, and superoxide dismutase in wheat shoot tended to increase first and then decrease, while the content of malondialdehyde decreased under low concentration of Cd stress and increased under high concentration of Cd stress. Exogenous melatonin significantly decreased the Cd content in wheat root and shoot to some extent. Moreover, when the Cd concentration was 100 μmol·L-1, the transfer coefficient of Cd in wheat seeding increased significantly, which indicated that exogenous melatonin not only reduced Cd absorption in wheat seeding, but also promoted Cd transfer in wheat seeding to some extent. However, when the Cd concentration increased, the higher concentration of melatonin had no significant effect on the transfer of Cd in wheat seeding.
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Key words:
- melatonin /
- Cd stress /
- wheat seedling /
- growth and physiological characteristic /
- heavy metal pollution
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