包包曲与平板曲理化指标及细菌群落结构研究
Study on the physicochemical indexes and bacterial community structure of bag Daqu and brick Daqu
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摘要: 检测包包曲和平板曲的理化指标，并结合高通量测序技术分析两种曲的细菌群落结构.结果表明：包包曲和平板曲的水分含量、酸度、淀粉含量等理化指标均在成品曲的标准范围之内，与包包曲相比，平板曲的水分含量和发酵力较高且差异显著，糖化力、液化力、酯化力、发酵力、酒化力均较高且差异极显著，酸度和氨基酸态氮含量均较低且差异极显著，淀粉含量差异不明显；以平均相对丰度0.50%为阈值，包包曲和平板曲共有的优势细菌门依次为变形菌门、厚壁菌门和放线细菌门，且平板曲的优势细菌门比包包曲多一个拟杆菌门；以平均相对丰度1.00%为阈值，包包曲的优势细菌属依次为假单胞菌属、泛菌属、不动杆菌属、明串珠菌属和乳杆菌属，平板曲的优势细菌属依次为乳杆菌属、明串珠菌属、泛菌属、克雷伯氏菌属、假单胞菌属、葡萄球菌属和乳球菌属，且首次从平板曲中发现巴那斯拉菌属.Abstract: The physicochemical indexes of bag Daqu and brick Daqu were studied. With high-throughput sequencing technology the bacterial community structure of bag Daqu and brick Daqu samples were analyzed. The results showed that the moisture content, acidity, starch content and other physicochemical indexes of brick Daqu and bag Daqu were within the standard range of finished Daqu. Compared with bag Daqu, the moisture content and fermentation power of brick Daqu were higher and the difference was significant;the saccharification power, liquefaction power, esterification power and fermentation power brick Daqu were higher, the acidity and starch content of brick Daqu were lower and the difference was very significant;the starch content was not much different. With the average relative abundance of 0. 05% as the threshold, the dominant bacteria of the bag Daqu and brick Daqu were Proteobacteria, Firmicutes and Actinobacteria. There was one more Bacteroidetes in the dominant bacteria of he brick Daqu than in bag Daqu. With the average relative abundance of 1. 00% as the threshold, the predominant bacterial genus of bag Daqu were Pseudomonas, Pantoea, Acinetobacter, Leuconostoc, Lactobacillus, and the predominant bacterial genus of brick Daqu were Lactobacillus, Leuconostoc, Pantoea, Klebsiella, Pseudomonas, Staphylococcus, Lactococcus. In this study, Barnesiella was discovered from the brick Daqu for the first time.
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