| 杜晓敏,王金花,朱鲁生,王军,杨莉莉,林琳.毒死蜱降解菌降解特性及其降解条件优化[J].农业环境科学学报,2020,39(10):2437-2445. |
| 毒死蜱降解菌降解特性及其降解条件优化 |
| Characteristics of a chlorpyrifos-degrading bacterial strain and optimization of its degradation conditions by response surface methodology |
| 投稿时间：2020-02-27 |
| DOI：10.11654/jaes.2020-0212 |
| 中文关键词: 毒死蜱 降解菌 降解特性 单因素试验 响应面分析法 |
| 英文关键词: chlorpyrifos bacterial strain degradation characteristics single-factor experiment response surface methodology |
| 基金项目:国家重点研发计划项目（2017YFD0800703，2016YFD0201203）；山东省自然科学基金项目（ZR2016JL029，JQ201711）；泰山学者工程专项 |
| 作者 | 单位 | E-mail | | 杜晓敏 | 山东农业大学资源与环境学院, 山东省高校农业环境重点实验室, 山东 泰安 271018 | | | 王金花 | 山东农业大学资源与环境学院, 山东省高校农业环境重点实验室, 山东 泰安 271018 | wjh@sdau.edu.cn | | 朱鲁生 | 山东农业大学资源与环境学院, 山东省高校农业环境重点实验室, 山东 泰安 271018 | | | 王军 | 山东农业大学资源与环境学院, 山东省高校农业环境重点实验室, 山东 泰安 271018 | | | 杨莉莉 | 山东农业大学资源与环境学院, 山东省高校农业环境重点实验室, 山东 泰安 271018 | | | 林琳 | 山东农业大学资源与环境学院, 山东省高校农业环境重点实验室, 山东 泰安 271018 | |
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| 中文摘要: |
| 为获得用于修复毒死蜱（Chlorpyrifos，CP）污染环境的高效降解菌，从大田土壤中分离、筛选出1株能降解毒死蜱的菌株，经形态特征和16S rDNA序列分析，该菌株为Bacillus属细菌，命名为H27。单因素降解条件实验初步获得毒死蜱降解的最适时间为48 h，最适温度为25℃，最适pH为7.0。降解酶定位实验结果表明，该降解菌产生的降解毒死蜱的代谢酶主要是胞内酶。为了提高降解率，采用Box-Behnken实验设计及响应面法分析，确定毒死蜱初始浓度为25 mg·L-1时，其最优降解条件为降解时间54 h、pH为7.2、温度为24℃，在此实验条件下毒死蜱的降解率为88.96%。 |
| 英文摘要: |
| In order to obtain a bacterial strain for bioremediating chlorpyrifos contamination, a bacterial strain capable of degrading chlorpyrifos was isolated from field soil. Based on its morphological characteristics and 16S rDNA sequence analysis, the strain was identified as Bacillus and named H27. Through single-factor degradation condition experiments, the results showed that its optimal degradation time was 48 h, optimal temperature was 25℃, and optimal pH was 7.0. Localization of the chlorpyrifos-degrading enzyme was conducted, and the results showed that chlorpyrifos-degrading enzymes were mainly intracellular enzymes. In order to improve the chlorpyrifos degradation rate, three factors were further optimized using the Box-Behnken design and response surface analysis. When the initial concentration of chlorpyrifos was 25 mg·L-1, the optimal conditions were as follows:Time of 54 h, pH of 7.2, and temperature of 24℃. Under these optimal conditions, the chlorpyrifos degradation rate reached 88.96%. This study provides information for the effective treatment of chlorpyrifos-contaminated soil. |
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