Abstract
Evidence is presented for complexation of guanosine 5′-monophosphate 2-methylimidazolide (2-MeImpG) with polycytidylate (poly(C)) at pH 8.0 and 23°C in the presence of 1.0 M NaCl and 0.2 M MgCl2 in water. The association of 2-McImpG with poly(C) was investigated using UV-vis spectroscopy as well as by monitoring the kinetics of the nucleophilic substitution reaction of the imidazole moiety by amines. The results of both methods are consistent with moderately strong poly(C) · 2-McImpG complexation and the spectrophotometric measurements allowed the construction of a binding isotherm with a concentration of 2-McImpG equal to 5.55 ± 0.15 mM at half occupancy. UV spectroscopy was employed to establish the binding of other guanosine derivatives on poly(C). These derivatives are guanosine 5′-monophosphate (5′GMP), guanosine 5′monophosphate imidazolide (ImpG), and guanosine 5′monophosphate morpholidate (morpG). Within experimental error these guanosine derivatives exhibit the same affinity for poly(C) as 2-McImpG.
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Kanavarioti, A., Hurley, T.B. & Baird, E.E. Affinity of guanosine derivatives for polycytidylate revisited. J Mol Evol 41, 161–168 (1995). https://doi.org/10.1007/BF00170668
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DOI: https://doi.org/10.1007/BF00170668