Antioxidant Activity of Polyphenolic Leaf Extract from Santolina chamaecyparissus L. (Asteraceae) and the Isolated Luteolin-7-O-Glucoside
Chahra Boudoukha *
Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University of Ferhat Abbas, Setif 1, Setif, Algeria.
Hamama Bouriche
Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University of Ferhat Abbas, Setif 1, Setif, Algeria.
Mahfuz Elmastas
Plant Research Laboratory-A, Department of Chemistry, Faculty of Art and Science, Gaziosmanpasa University, 60240 Tokat, Turkey.
Huseyin Aksit
Plant Research Laboratory-A, Department of Chemistry, Faculty of Art and Science, Gaziosmanpasa University, 60240 Tokat, Turkey.
Omer Kayir
Plant Research Laboratory-A, Department of Chemistry, Faculty of Art and Science, Gaziosmanpasa University, 60240 Tokat, Turkey.
Nusret Genc
Plant Research Laboratory-A, Department of Chemistry, Faculty of Art and Science, Gaziosmanpasa University, 60240 Tokat, Turkey.
Abderrahmane Senator
Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University of Ferhat Abbas, Setif 1, Setif, Algeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: The present study aimed to investigate the chemical constituents and antioxidant potential of Santolina chamaecyparissus polyphenolic extract (SCPE).
Materials and Methods: The extract containing phenolic compounds, was extracted with ethyl acetate and luteolin-7-O-glucoside was separated and purified using flash chromatography on silica gel column. This isolated compound was identified according to its physicochemical properties and spectral data (UV, LC–TOF-MS, 1H NMR, 13C NMR and 2D NMR). In vitro antioxidant activity of the isolated compound and SCPE was investigated using DPPH•, ABTS+, reducing power, superoxide anion radical scavenging and β-carotene bleaching assays.
Results: Results showed that SCPE and luteolin-7-O-glucoside exhibited significant antioxidant activity. The antioxidant activity of SCPE was comparable to that of luteolin-7-O-glucoside. Luteolin-7-O-glucoside was isolated for the first time from Santolina chamaecyparissus polyphenolic extract.
Conclusion: Results of the present investigation clearly indicate that SCPE and isolated compound (luteolin-7-O-glucoside) have powerful antioxidant capacity against various antioxidant systems in vitro
Keywords: Antioxidant, flavone, luteolin-7-o-glucoside, polyphenols, Santolina chamaecyparissus.