DEVELOPMENT OF A PRACTICABLE METHOD FOR ISOLATION AND IDENTIFICATION OF DENGUE VIRUSES IN DEVELOPPING COUNTRIES

Masaru NAWA; Cleotilde A. TORRES; Fem J.E. PALADIN; Thelma E. TUPASI; Yoshinori KANEKO

doi:10.7883/yoken1952.40.79

Masaru NAWA, Cleotilde A. TORRES, Fem J.E. PALADIN, Thelma E. TUPASI, Yoshinori KANEKO

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JOURNAL FREE ACCESS

1987 Volume 40 Issue 2 Pages 79-82

DOI https://doi.org/10.7883/yoken1952.40.79

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Abstract

We sought a practicable method for isolation and identification of dengue viruses in South-East Asia. We compared two mosquito cell lines, C6/36 and TRA-284-SFG, for virus isolation and two identification methods, immunofluorescent staining of infected cells with serotype-specific mouse monoclonal antibodies and a sandwich-type enzyme-linked immunosorbent assay with conventional mouse hyperimmune ascitic fluids. We found that the combination of TRA-284-SFG cells and ELISA is a useful and feasible method in developping countries.

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