Three new species of Axinulus (Bivalvia: Thyasiridae) from the Japan and Kuril-Kamchatka trenches and abyssal zone of the northern Pacific Ocean

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Zoological Science

Introduction

In recent years, international expeditions organized by the Russian Federation and Germany have sampled the deep-sea benthic fauna in an extensive region of the northwestern Pacific Ocean with depths greater than 3,000 m. The studies were focused on the composition and distribution of the benthic fauna in the deep-sea basins of the Seas of Japan and Okhotsk, the Kuril-Kamchatka Trench, and at the abyssal plain of the Pacific Ocean adjacent to the Kuril-Kamchatka Trench (Malyutina & Brandt, 2013; Brandt & Malyutina, 2015; Malyutina, Chernyshev & Brandt, 2018; Brandt et al., 2019). Benthic macrofauna rich in species number and abundance was found in these deep-sea ecosystems, with bivalves being one of the dominant groups of animals (Brandt et al., 2013, 2015, 2018, 2020; Kamenev, 2013, 2014, 2015, 2018a, 2018b, 2018c, 2019; Kamenev et al., 2022). Among the bivalve fauna of all the studied deep-sea areas of the northwestern Pacific Ocean, the Thyasiridae was the most species-rich family and many thyasirids were the dominant species in terms of abundance in the benthic macrofaunal communities (Kamenev, 2013, 2015, 2018a, 2019; Kamenev et al., 2022). At least 14 thyasirid species were found at depths greater than 3,000 m in the abyssal and hadal zones of the northwestern Pacific Ocean. They have not been identified to the species level and are most likely new to science. Moreover, previous studies of the northwestern Pacific deep-sea fauna revealed many species of thyasirids in various oceanic trenches (Belyaev & Mironov, 1977; Belyaev, 1989; Okutani, Fujikura & Kojima, 1999; Allen, 2015), most of them remain so far unidentified to the species level (Belyaev, 1989). Thus, despite the great importance of thyasirids in the functioning of the deep-sea ecosystems of the northwestern Pacific Ocean, the abyssal and hadal thyasirid fauna in the region is almost not studied, except three species (Axinulus hadalis (Okutani, Fujikura & Kojima, 1999), Axinulus philippinensis Allen, 2015, and Thyasira kaireiae (Okutani, Fujikura & Kojima, 1999)), which were found in the Japanese and Philippine trenches (Okutani, Fujikura & Kojima, 1999; Allen, 2015).

Several years ago, the author of this article started a study of unidentified deep-sea species of the Thyasiridae found in the northwestern Pacific Ocean. As a result of this work, six new species have been described from the abyssal and hadal zones of the Sea of Okhotsk and the Bering Sea, the Kuril-Kamchatka and Japan trenches, as well as the oceanic plain adjacent to these trenches (Kamenev, 2020, 2023). The present article is a continuation of the study of northwestern Pacific deep-sea thyasirids and presents a description of three new species of the genus Axinulus that were found in the Japan and Kuril-Kamchatka trenches, as well as in the abyssal zone of other deep-sea regions of the northern Pacific Ocean.

Materials and Methods

Material studied

The material examined in this study was collected from 1954 to 1990 by expeditions of the P.P. Shirshov Institute of Oceanology, Russian Academy of Sciences, Moscow (IO RAS) in the hadal zone of the Kuril-Kamchatka Trench and from the Pacific abyssal plain adjacent to the Kuril-Kamchatka Trench (RV Vityaz, cruise no. 19, August 17–October 29, 1954; RV Vityaz, cruise no. 39, July 7–September 13, 1966), in the hadal zone of the Japan Trench (RV Vityaz, cruise no. 59, May 26–July 5, 1976), in the Gulf of Alaska (RV Vityaz, cruise no. 45, April 23–July 10, 1969), and from the bottom of the Commander Basin (Bering Sea) (RV Akademik Mstislav Keldysh, cruise no. 22, July 25–October 27, 1990), as well as by the German-Russian deep-sea expeditions KuramBio (RV Sonne, cruise no. 223, July 21–September 7, 2012) and KuramBio II (RV Sonne, cruise no. 250, August 16–September 29, 2016) from the Pacific abyssal plain adjacent to the Kuril-Kamchatka Trench and in the hadal zone of the Kuril-Kamchatka Trench; and by the Russian-German deep-sea expedition SokhoBio (RV Akademik M.A. Lavrentyev, cruise no. 71, July 6–August 6, 2015) in the abyssal zone of the Pacific slope of the Kuril Islands. The new species of Axinulus were found among the 72 samples of benthic fauna at depths of 3,200–9,583 m. The methods for the sample collecting and fixing have been in detail described previosly (Monin, 1983; Brandt & Malyutina, 2015; Malyutina, Chernyshev & Brandt, 2018; Brandt et al., 2020; Kamenev, 2020, 2023). The samples obtained during the expeditions of the IO RAS were stored in the IO RAS Ocean Benthic Fauna collection. The samples collected by the KuramBio, KuramBio II, and SokhoBio expeditions were stored at the Museum (MIMB) of A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences (NSCMB FEB RAS), Vladivostok, Russia. The type and other materials of the new species were deposited at the MIMB, IO RAS, and Senckenberg Museum Frankfurt, Germany (SMF).

Additional material was also examined for the comparison purposes: Axinulus careyi Bernard, 1979 (holotype, LACM 1990 (Natural History Museum of Los Angeles County, Los Angeles, CA, USA)); paratype, SBNHM 55440 (Santa Barbara of the Natural History Museum, Santa Barbara); Axinulus kelliaeformis Okutani, 1962 (holotype, NSMT Mo 69697 (National Museum of Nature and Science, Tsukuba, Japan)); Axinulus thackergeigeri Valentich-Scott & Coan, 2012 in Coan & Valentich-Scott, 2012 (holotype, SBNHM 149742; paratype, SBNHM 149743); Axinulus obliquus Okutani, 1968 (holotype, NSMT Mo 69719, and photos of paratype and additional specimens, NSMT Mo 69720 (photos by Dr. H. Saito)); Clausina croulinensis Jeffreys, 1847 (neotype, USNM 62048 (National Museum of Natural History, Washington, D.C., USA), photos from USNM Web site); Cryptodon (Axinulus) brevis Verrill & Bush, 1898 (holotype, USNM 159873, photos from USNM Web site); Cryptodon (Axinulus) simplex Verrill & Bush, 1898 (holotype, USNM 159888, photos from USNM Web site); Cryptodon equalis Verrill & Bush, 1898 (holotype, USNM 74302, photos from USNM Web site); Maorithyas hadalis Okutani, Fujikura & Kojima, 1999 (paratype, NSMT Paratype C Mo 71432-c). Information and photos from USNM Web site provided with the permission of the National Museum of Natural History, Smithsonian Institution, 10th and Constitution Ave. N.W., Washington, DC 20560-0193 (http://www.nmnh.si.edu/).

Methods

Shell length (L), height (H), anterior end length (A), and shell width (W) were measured with the ocular micrometer at the accuracy level of 0.1 mm (Fig. 1). The ratios H/L, A/L, and W/L were determined.

Placement of shell measurements.

Figure 1: Placement of shell measurements.

Abbreviations: L, shell length; H, height; A, anterior end length; W, shell width.

The methods for the sample preparation and the shell morphology study using the scanning microscopy as well as the gross anatomy analysis of the preserved alive-taken specimens have been described by Kamenev (2020, 2023). The microscopic studies of the new species described here were carried out at the Far Eastern Center of Electron Microscopy of the NSCMB FEB RAS. To describe the shell morphology and body anatomy of the new species belonging to the genus Axinulus, the terms proposed by Payne & Allen (1991) and Oliver & Killeen (2002) were used.

Nomenclatural acts

The electronic version of this article in Portable Document Format (PDF) will represent a published work according to the International Commission on Zoological Nomenclature (ICZN), and hence the new names contained in the electronic version are effectively published under that Code from the electronic edition alone. This published work and the nomenclatural acts it contains have been registered in ZooBank, the online registration system for the ICZN. The ZooBank LSIDs (Life Science Identifiers) can be resolved and the associated information viewed through any standard web browser by appending the LSID to the prefix http://zoobank.org/. The LSID for this publication is: urn:lsid:zoobank.org:pub:0AE8C4E1-0F13-478E-81E9-287980139049. The online version of this work is archived and available from the following digital repositories: PeerJ, PubMed Central SCIE and CLOCKSS.

Results

Systematics

Class Bivalvia

Order Lucinoida Gray, 1854

Superfamily Thyasiroidea Dall, 1900 (1895)

Family Thyasiridae Dall, 1900 (1895)

Genus Axinulus Verrill & Bush, 1898

Type species (by original designation): Cryptodon (Axinulus) brevis Verrill & Bush, 1898

Diagnosis (after Kamenev, 2020): Shell small (<10 mm), equilateral or subequilateral, ovate to ovate-rhomboidal, slightly higher than long; margins entire, without any posterior sinus, but sometimes with weak angulation of posterior margin; posterior sulcus absent or weak. Lunule variably expressed, indistinct to deep; escutcheon and auricle sometimes present. Hinge plate edentulous, sometimes with a small swelling. Ligament sunken, sometimes visible externally. Ctenidium of a single demibranch, foot vermiform, lateral body pouches without or with distinct lobes.

Remarks: Despite that the WoRMS (WoRMS Editorial Board, 2023) currently lists 13 species of the genus Axinulus, some authors (Zelaya, 2010; Allen, 2015; Oliver, 2015; Kamenev, 2020) recognize only eight species as belonging to the genus (Axinulus alleni (Carrozza, 1981), Axinulus brevis (Verrill & Bush, 1898), Axinulus croulinensis (Jeffreys, 1847), Axinulus antarcticus Zelaya, 2010, A. philippinensis, Axinulus subequatorius (Payne & Allen, 1991), Axinulus oliveri Kamenev, 2020, and Axinulus roseus Kamenev, 2020). Therefore, the new species described herein were compared only with the above species. Assignment the other five species to the genus Axinulus is doubtful (Oliver, 2015; Kamenev, 2020).

Axinulus krylovae sp. nov.

(Figs. 25, Tables 1 and 2)

Axinulus krylovae sp. nov.

Figure 2: Axinulus krylovae sp. nov.

(A–D) Holotype (IO RAS BIV00984), exterior and dorsal views, and sculpture of central shell part, shell length 4.9 mm. (E and F) Paratype (IO RAS BIV00985), exterior and oblique dorsal views, shell length 5.0 mm. (G–J) Exterior and interior views of right and left valves, and hinge plates of both valves, valves length 4.4 mm. (K–M) Variability of shell shape, exterior view of right valves of specimens from holotype locality: (K) shell length 3.5 mm ; (L) shell length 4.3 mm; (M) shell length 4.0 mm. Abbreviation: R, radial, whitish ray. Scale bar: D, F, I, J = 1 mm.
Scanning electron micrographs of Axinulus krylovae sp. nov.

Figure 3: Scanning electron micrographs of Axinulus krylovae sp. nov.

(A) Dorsal view of both valves. (B) Dorsal view of posterodorsal margin. (C) Lunule. (D) Oblique dorsal view of lunule. (E–H) Specimen from the Japan Trench, depth 7,540 m: (E) Exterior view of right valve. (F) Sculpture of beak region. (G and H) Sculpture of central shell part. (I–L) Specimen from the Kuril-Kamchatka Trench, depth 8,734 m: (I) Exterior view of right valve. (J) Sculpture of beak region. (K) Sculpture of ventral shell part. (L) Sculpture of central shell part. (M–P) Prodissoconch of specimen from the Japan Trench. (Q–T) Prodissoconch of specimen from the Kuril-Kamchatka Trench. Scale bars: A, C–E, I = 1 mm; B = 500 μm; F–H, J–L, = 100 µm; M–T = 10 µm.
Scanning electron micrographs of Axinulus krylovae sp. nov.

Figure 4: Scanning electron micrographs of Axinulus krylovae sp. nov.

(A–H) Specimen from the Kuril-Kamchatka Trench, depth 8,700 m: (A and B) Interior and ventral views of right valve. (C and D) Hinge plate and ligamental groove of right valve. (E and F) Interior and ventral views of left valve. (G and H) Hinge plate and ligamental groove of left valve. (I–P) Specimen from the Japan Trench, depth 7,540 m: (I and J) Interior and ventral views of right valve. (K and L) Hinge plate and ligamental groove of right valve. (M and N) Interior and ventral views of left valve. (O and P) Hinge plate and ligamental groove of left valve. (Q–T) Exterior and interior views of valves of young specimens: (Q and R) Specimen from the Kuril-Kamchatka Trench, depth 8,700 m. (S and T) Specimen from the abyssal plain adjacent to the Kuril-Kamchatka Trench, depth 5,125 m. Scale bars: A, B, E, F, I, J, M, N, Q, R = 500 µm; C, D, G, H, K, L, O, P, S, T = 100 µm.
Axinulus krylovae sp. nov.

Figure 5: Axinulus krylovae sp. nov.

(A) Exhalant aperture. (B) Gross anatomy after removal of right valve and mantle. (C) Gross anatomy after further removal of right ctenidium. (D and E) Gross anatomy after further removal of right lateral body pouch. (F) Labial palps, anterior pedal and adductor muscles. (G) Gross anatomy after further removal of right kidney. (H) Kidney with orange granules. (I) Digestive system. (J) Posterior view of kidneys, ctenidium, and lateral body pouches of live specimen after removal shell and mantle. (K) Foot of live specimen. (L) Egg (photo by Dr. O.V. Yurchenko (NSCMB FEB RAS)). (M) Gross anatomy after removal of right valve, mantle, right kidney, and posterior adductor muscle. (N) Gross anatomy after further removal of right ctenidium and mantle edge. (O) Gross anatomy after further removal of right lateral body pouch and anterior adductor muscle. (P) Digestive system. Abbreviations: AA, anterior adductor muscle; APR, anterior pedal retractor muscle; EA, exhalant aperture; F, foot; HG, hind gut; ID, inner demibranch; K, kidney; L, ligament; LBP, lateral body pouch; LP, labial palps; ME, mantle edge; MG, mid gut; NP, neck of lateral body pouch; PA, posterior adductor muscle; PPR, posterior pedal retractor muscle; S, stomach. Scale bars: A–K, M–P = 1 mm; L = 100 µm.
Table 1:
Additional material of Axinulus krylovae sp. nov. examined in the present study.
Ship, cruise no. Station Date Start End Depth (m) Gear N Depository
Latitude °N Longitude °E Latitude °N Longitude °E
Japan Trench
Vityaz 45 6151 28.06.1969 37°41.5′ 143°54.3′ 7,370 ST 1 IORAS BIV00980
Vityaz 59 7500 21.06.1976 37°38′ 143°58′ 7,370–7,350 GT 1 IORAS BIV00992
7503 23.06.1976 36°44′ 143°19′ 7,540 GT 74 IORAS BIV00986
7511 27.06.1976 38°38′ 144°06′ 7,500 OG 1 IORAS BIV00987
Kuril-Kamchatka Trench
Vityaz 19 3168 04.10.1954 45°41.7′ 152°36.7′ 6,150 OG 8 IORAS BIV00981
Vityaz 39 5615 03.08.1966 45°56′ 153°28′ 8,060–8,135 ST 2 IORAS BIV00982
Sonne 250 17 22.08.2016 45°51.514′ 153°50.581′ 45°51.401′ 153°50.406′ 8,183–8,184 EBS 6 MIMB 45900
19 23.08.2016 45°51.566′ 153°50.451′ 45°51.412′ 153°50.215′ 8,189–8,187 EBS 2 MIMB 45901
25 25.08.2016 45°55.235′ 152°47.464′ 6,068 GKG 3 MIMB 45902
28 26.08.2016 45°53.959′ 152°45.838′ 45°54.520′ 152°47.204′ 6,087–6,047 EBS 1 MIMB 45903
29 26.08.2016 45°56.587′ 152°54.251′ 45°56.570′ 152°54.499′ 6,204–6,202 AGT 2 MIMB 45904
30 27.08.2016 45°56.821′ 152°51.185′ 45°56.834′ 152°50.943′ 6,168–6,164 EBS 8 MIMB 45905
37 28.08.2016 45°38.604′ 152°55.911′ 7,136 GKG 1 MIMB 45906
40 29.08.2016 45°40.686′ 152°57.374′ 45°40.839′ 152°57.687′ 7,060–7,055 EBS 1 MIMB 45907
42 30.08.2016 45°40.173′ 152°57.457′ 45°40.263′ 152°57.638′ 7,122–7,120 EBS 6 MIMB 45908
49 04.09.2016 45°28.752′ 153°11.649′ 8,739 GKG 3 MIMB 45909
51 05.09.2016 45°28.748′ 153°11.654′ 8,737 MUC 1 MIMB 45910
52 05.09.2016 45°29.347′ 153°11.415′ 45°29.187′ 153°11.138′ 8,738–8,699 EBS 24 MIMB 45911
54 06.09.2016 45°28.139′ 153°10.624′ 45°28.125′ 153°10.109′ 8,701–8,735 AGT 14 MIMB 45912
55 07.09.2016 45°29.491′ 153°12.540′ 45°29.580′ 153°12.240′ 8,740–8,735 EBS 11 MIMB 45913
67 10.09.2016 45°12.944′ 152°42.844′ 9,495 GKG 1 MIMB 45914
75 12.09.2016 44°39.883′ 151°28.136′ 8,221 GKG 9 MIMB 45915
77 13.09.2016 45°13.892′ 152°50.774′ 45°14.219′ 152°49.956′ 9,577–9,583 EBS 11 MIMB 45916
89 16.09.2016 44°39.325′ 151°27.340′ 44°39.053′ 151°27.343′ 8,215–8,217 EBS 8 MIMB 45917
90 17.09.2016 44°41.759′ 151°26.554′ 44°41.992′ 151°26.321′ 8,271–8,273 AGT 24 MIMB 45918
94 18.09.2016 44°06.852′ 151°25.539′ 6,531 GKG 11 MIMB 45919
97 18.09.2016 44°06.668′ 151°24.878′ 44°06.942′ 151°24.888′ 6,551–6,561 EBS 13 MIMB 45920
98 19.09.2016 44°06.152′ 151°25.705′ 44°06.253′ 151°25.935′ 6,441–6,442 AGT 12 MIMB 45921
100 20.09.2016 44°12.378′ 150°39.053′ 9,305 GKG 3 MIMB 45922
103 21.09.2016 44°12.499′ 150°39.055′ 44°12.502′ 150°37.258′ 9,301–9,431 AGT 1 MIMB 45923
105 22.09.2016 44°12.391′ 150°36.006′ 9,540 GKG 1 MIMB 45924
Kuril-Kamchatka Trench area and abyssal plain adjacent to trench (depth less than 6,000 m)
Vityaz 19 3102 22.08.1954 41°16′ 147°27.7′ 5,210 OG 1 IORAS BIV00983
Sonne 223 1-5 29.07.2012 43°58.17′ 157°19.88′ 5,410 GKG 1 MIMB 45925
2-5 02.08.2012 46°13.99′ 155°33.10′ 4,869 GKG 1 MIMB 45926
2-9 02.08.2012 46°13.60′ 155°33.42′ 46°14.93′ 155°32.57′ 4,866–4,860 EBS 3 MIMB 45927
2-11 03.08.2012 46°13.69′ 155°33.29′ 46°14.87′ 155°32.49′ 4,869–4,861 AGT 5 MIMB 45928
3-4 04.08.2012 47°14.32′ 154°42.26′ 4,982 GKG 6 MIMB 45929
3-9 05.08.2012 47°13.83′ 154°41.88′ 47°14.87′ 154°43.18′ 4,988–4,998 EBS 3 MIMB 45930
4-4 07.08.2012 46°57.97′ 154°32.49′ 5,766 GKG 3 MIMB 45931
5-4 10.08.2012 43°35.01′ 153°58.09′ 5,379 GKG 1 MIMB 45932
6-4 13.08.2012 42°28.98′ 153°59.97′ 5,297 GKG 1 MIMB 45933
7-4 16.08.2012 43°02.31′ 152°59.16′ 5,222 GKG 7 MIMB 45934
7-5 16.08.2012 43°02.24′ 152°59.09′ 5,223 GKG 3 MIMB 45935
7-9 17.08.2012 43°02.87′ 152°59.45′ 43°01.50′ 152°58.35′ 5,216–5,221 EBS 3 MIMB 45936
7-10 17.08.2012 43°02.78′ 152°59.30′ 43°01.65′ 152°58.45′ 5,217–5,223 EBS 20 MIMB 45937
8-4 19.08.2012 42°14.57′ 151°43.51′ 5,130 GKG 1 MIMB 45938
8-5 19.08.2012 42°14.57′ 151°43.51′ 5,130 GKG 5 MIMB 45939
8-9 20.08.2012 42°14.69′ 151°44.05′ 42°14.26′ 151°42.49′ 5,127 EBS 18 MIMB 45940
8-12 21.08.2012 42°14.73′ 151°44.38′ 42°14.32′ 151°42.94′ 5,112–5,126 EBS 7 MIMB 45941
9-5 23.08.2012 40°34.96′ 151°00.07′ 5,401 GKG 1 MIMB 45942
9-9 23.08.2012 40°35.49′ 150°59.92′ 40°34.25′ 150°59.91′ 5,399–5,398 EBS 2 MIMB 45943
9-10 24.08.2012 40°36.13′ 151°00.07′ 40°35.31′ 151°00.12′ 5,406–5,404 AGT 3 MIMB 45944
9-12 25.08.2012 40°35.40′ 150°59.84′ 40°34.27′ 150°59.00′ 5,398 EBS 1 MIMB 45945
10-5 26.08.2012 41°11.99′ 150°05.75′ 5,251 GKG 1 MIMB 45946
10-12 27.08.2012 41°11.70′ 150°05.56′ 41°13.03′ 150°05.71′ 5,250–5,255 EBS 4 MIMB 45947
11-4 29.08.2012 40°12.86′ 148°05.92′ 5,348 GKG 5 MIMB 45948
11-9 30.08.2012 40°13.26′ 148°06.24′ 40°12.37′ 148°05.43′ 5,348–5,350 EBS 5 MIMB 45949
11-11 30.08.2012 40°13.55′ 148°06.77′ 40°12.90′ 148°06.20′ 5,349–5,352 AGT 1 MIMB 45950
11-12 31.08.2012 40°13.10′ 148°06.45′ 40°12.10′ 148°05.53′ 5,351–5,348 EBS 6 MIMB 45951
12-2 31.08.2012 39°43.43′ 147°09.98′ 5,243 GKG 7 MIMB 45952
12-4 01.09.2012 39°43.80′ 147°10.16′ 39°42.49′ 147°09.37′ 5,224–5,215 EBS 3 MIMB 45953
Sonne 250 6 18.08.2016 43°49.197′ 151°45,609′ 5,497 GKG 1 MIMB 45954
8 19.08.2016 43°48.593′ 151°46.433′ 43°48.598′ 151°46.477′ 5,107 EBS 13 MIMB 45955
10 20.08.2016 43°48.602′ 151°47.124′ 43°48.455′ 151°47.171′ 5,352–5,104 EBS 7 MIMB 45956
61 08.09.2016 45°09.997′ 153°45.419′ 5,741 GKG 5 MIMB 45957
65 09.09.2016 45°10.226′ 153°44.178′ 45°10.160′ 153°44.052′ 5,734–5,752 EBS 1 MIMB 45958
86 15.09.2016 45°01.202′ 151°06.008′ 45°01.371′ 151°06.001′ 5,572–5,530 AGT 19 MIMB 45959
87 16.09.2016 45°01.383′ 151°05.527′ 45°01.651′ 151°05.522′ 5,496–5,478 EBS 1 MIMB 45960
DOI: 10.7717/peerj.15543/table-1

Note:

GT, Galathea trawl; OG, Okean grab (0.25 m2); ST, Sigsbee trawl; GKG, giant box corer (0.25 m2); EBS, epibenthic sledge; AGT, Agassiz trawl; N, number of live specimens.

Table 2:
Axinulus krylovae sp. nov.
Shell measurements (mm), indices and summary statistics of indices.
Depository L H A W H/L A/L W/L
Paratype IORAS BIV00985 5.1 5.5 2.3 3.5 1.078 0.451 0.686
Paratype IORAS BIV00985 5.0 5.4 1.9 3.2 1.080 0.380 0.640
Holotype IORAS BIV00984 4.9 5.3 2.0 3.4 1.082 0.408 0.694
IORAS BIV00986 4.8 5.5 2.2 3.3 1.146 0.458 0.688
IORAS BIV00986 4.8 5.2 1.8 3.1 1.083 0.375 0.646
IORAS BIV00986 4.7 4.8 2.1 3.0 1.021 0.447 0.638
Paratype MIMB 45898 4.7 5.3 1.8 3.6 1.128 0.383 0.766
IORAS BIV00986 4.6 4.6 1.7 2.9 1.000 0.370 0.630
Paratype IORAS BIV00985 4.5 5.1 1.9 3.2 1.133 0.422 0.711
Paratype IORAS BIV00985 4.5 4.8 1.7 3.1 1.067 0.378 0.689
IORAS BIV00986 4.3 4.5 1.9 3.1 1.047 0.442 0.721
Paratype MIMB 45899 4.2 4.7 1.9 3.0 1.119 0.452 0.714
Paratype SMF 372429 4.1 4.3 1.6 2.8 1.049 0.390 0.683
IORAS BIV00986 4.2 5.0 1.8 3.0 1.190 0.429 0.714
IORAS BIV00986 4.2 4.4 1.5 3.0 1.048 0.357 0.714
IORAS BIV00986 4.2 4.6 2.0 2.9 1.095 0.476 0.690
IORAS BIV00986 4.1 4.4 1.6 2.8 1.073 0.390 0.683
IORAS BIV00986 4.0 4.1 1.6 2.4 1.025 0.400 0.600
IORAS BIV00986 3.9 4.1 1.5 2.5 1.051 0.385 0.641
IORAS BIV00986 3.8 4.3 1.7 2.6 1.132 0.447 0.684
IORAS BIV00986 3.8 4.0 1.8 2.4 1.053 0.474 0.632
IORAS BIV00986 3.7 3.8 1.3 2.4 1.027 0.351 0.649
IORAS BIV00986 3.7 3.9 1.5 2.4 1.054 0.405 0.649
IORAS BIV00986 3.6 4.1 1.6 2.4 1.139 0.444 0.667
IORAS BIV00986 3.6 3.8 1.4 2.4 1.056 0.389 0.667
IORAS BIV00986 3.5 3.7 1.5 2.3 1.057 0.429 0.657
IORAS BIV00986 3.5 3.5 1.5 2.2 1.000 0.429 0.629
IORAS BIV00986 3.5 3.8 1.4 2.3 1.086 0.400 0.657
IORAS BIV00986 3.4 3.7 1.4 2.1 1.088 0.412 0.618
IORAS BIV00986 3.3 3.7 1.6 2.2 1.121 0.485 0.667
IORAS BIV00986 3.3 3.7 1.5 2.2 1.121 0.455 0.667
IORAS BIV00986 2.4 2.9 0.9 1.6 1.208 0.375 0.667
Statistics L H A W H/L A/L W/L
Mean 1.083 0.415 0.671
SE 0.039 0.032 0.028
SD 0.050 0.037 0.035
Min 1.000 0.351 0.600
Max 1.208 0.485 0.766
DOI: 10.7717/peerj.15543/table-2

Note:

L, shell length; H, height; A, anterior end length; W, width.

Axinulus sp.: Kamenev, 2015, p. 191.

Genaxinus” sp. 1: Kamenev, 2019, p. 6, 7.

urn:lsid:zoobank.org:act:F3D588B6-84FD-44A7-99BF-7FB53AC893F3

Type material and locality: Holotype (IORAS BIV00984), Japan Trench, Pacific Ocean (36°44′N, 143°19′E), 7,540 m, Galathea trawl, Coll. F.A. Pasternak, 23-VI-1976 (RV Vityaz, cruise no. 59, stn. 7503); paratypes (4) (IORAS BIV00985) and paratype (MIMB 45898) from holotype locality; paratype (MIMB 45899) and paratype (SMF 372429), Kuril-Kamchatka Trench, Pacific Ocean (45°56.821′N, 152°51.185′E–45°56.834′N, 152°50.943′E), 6,168–6,164 m, epibenthic sledge, Coll. A. Brandt, 27-VIII-2016 (RV Sonne, cruise no. 250, stn. 30).

Other material examined: 438 live specimens (Table 1).

Diagnosis: Shell medium in size (to 5.2 mm in length), elongated ovate to rhomboidal, with two radiating, whitish, elongated triangular rays extending from beaks to anteroventral and posteroventral margins. Sculpture of closely spaced commarginal riblets forming weak undulations and conspicuous, closely spaced, radial ribs and rays as slightly convex overlap areas of varying width over commarginal riblets. Posterior folds and sulcus absent. Escutcheon and auricle absent. Lunule as a crest, raised, wide, weakly defined. Ligament sunken, only slightly visible externally, long. Prodissoconch small (length 131–158 µm), irregularly convex, with distinct tubercle in anterior part; initial part densely sculptured, chaotically arranged, short, curved plicae and numerous, radial, thin folds. Adductor muscle scars distinct, outline elongate-triangular, raised above inner shell surface because of thickening of shell. Lateral body pouches large, simple, without numerous distinct lobes.

Description. Shell medium in size (to 5.2 mm in length and 5.7 mm in height), strongly inflated (W/L = 0.671 ± 0.028), slightly higher than long (H/L = 1.083 ± 0.039); elongated ovate to rhomboidal, equivalve, subequilateral, white, thick, with two curved, radial, whitish, elongated triangular, opaque rays extending from beaks to anteroventral and posteroventral margins, respectively, formed by internal thickening shell; patches of silty deposit adhering to anteriodorsal and posterior shell margins (Fig. 2 and Table 2). Periostracum thin, colorless, translucent, adherent. Dissoconch sculptured with thin, closely spaced, commarginal riblets forming weak, narrow, irregular, undulations and conspicuous, closely spaced, radial ribs and rays with overlap areas of varying width over commarginal riblets. Beaks small, raised, prosogyrate, curved slightly inwards, slightly anterior to midline (A/L = 0.415 ± 0.032) (Fig. 3 and Table 2). Anterodorsal shell margin convex, steeply sloping from beaks, forming a rounded angle at transition to anterior margin. Anterior margin curved, smoothly transitioning to ventral margin. Ventral margin strongly curved, sometimes slightly angulate. Posterodorsal margin slightly convex, steeply sloping from beaks, smoothly transitioning to posterior margin. Posterior margin slightly curved or straight, smoothly transitioning to ventral margin. Posterior folds and sulcus absent but posterior shell area a little flattened. Escutcheon and auricle absent. Lunule as a crest, raised, long, wide, weakly defined, demarcated by weak, long, rounded ridges along entire anterodorsal shell margin (Figs. 3C and 3D). Ligament opisthodetic, sunken, slightly visible externally as a narrow strip between valves, thick, almost straight, long, about half the length of posterodorsal shell margin, lying in deep, slightly curved, wide groove at surface of hinge plate (Fig. 4). Prodissoconch small (length 131–158 µm), ovate in outline, slightly drawn out anteriorly, irregularly convex, flattened anteriorly, with a distinct tubercle and shallow depression in initial part (Figs. 3M3T). Tubercle and depression of prodissoconch sculptured with numerous, densely spaced, short and chaotically arranged folds, forming a rounded area; numerous short, thin, radial folds of about same length radiating from this area; remaining surface of prodissoconch smooth or slightly bumpy (Fig. 3N). Hinge plate thickened, with a small peg under beak in each valve and long, deep ligamental groove (Fig. 4). Adductor muscle scars distinct, long, elongated triangular in outline, extending into umbonal cavity, bearing thin, indistinct, radial striation; shell strongly thickened in scar area, thus rendering muscle scars raised above inner shell surface. Posterior adductor scar narrow, straight; anterior adductor scar curved, approximately twice as wide as posterior (Fig. 4).

Gross anatomy: Mantle thin; margins thickened and unfused except limited interconnection at the posterior ventral margin with a small exhalant aperture below the posterior adductor muscle (Fig. 5A). Anterior adductor muscle elongated, two times longer than posterior adductor, slightly curved parallel to anterodorsal shell margin, dorsal part narrower than ventral part. Posterior adductor muscle small, oval. Ctenidium thin, narrow, consisting of a single inner demibranch with fully reflected filaments (up to 50 filaments in large specimens more than 4.7 mm in length). Demibranch not covering lateral body pouches and consisting of both ascending and descending lamellae; ascending lamellae slightly shorter than descending lamellae. Labial palps small (to 150 µm), triangular, located at proximal end of longer (more than 1 mm) oral grooves extending to antero-ventral corner of gill (Figs. 5B5F). Lateral pouches large (Figs. 5B, 5C, 5J, 5M and 5N), dorsoventrally elongated, oval in outline, with undulated margins and a few small, slightly projecting lobes along different margins; each pouch connecting to body by a wide neck (Figs. 5D5F). Kidneys large, elongated, occupying a posterodorsal position between posterior adductor muscle and heart, containing numerous, bright orange, large (to 80 µm in diameter), different-size granules (Figs. 5D, 5J and 5H). Gonad occupying inner side of lateral pouches. Sexes are separate. Eggs oval or polygonal (up to 120–130 µm in length after fixation) (Fig. 5L). Alimentary system with short esophagus leading to a relatively large, elongate stomach; combined style sac and strongly curved midgut forming a deep and narrow loop between neck of lateral pouches and kidney; hind gut forming an anterior, deep, and wide loop dorsal to style sac, running posteriorly dorsal to kidney and posterior adductor muscle, opening at ventral side of posterior adductor muscle (Figs. 5I and 5P). Foot long, vermiform, depending upon its state of contraction, may either form coil within mantle cavity or form a relatively short, curved steam with bulbous tip; surface of bulbous portion with densely spaced papillae (Figs. 5D, 5K and 5N). Heel absent. Anterior and posterior pedal retractors short, narrow (Figs. 5D and 5F).

Variability: The shell shape and proportions, the degree of slope of anterodorsal and posterodorsal shell margins and curving of all shell margins vary significantly among different sized specimens (Figs. 24 and Table 2). Some specimens have a shell rather elongated dorsoventrally and ovate in outline. In some specimens, the shell is more rounded and angulate, the anterodorsal and posterodorsal margins are sloped more gently from beaks, the anterodorsal margin is more convex and the posterodorsal margin is almost straight, the ventral margin is more curved and slightly drawn out anteriorly. Moreover, in thicker-shelled specimens the hinge plate is wider and the inner thickenings of the shell in the аdductor muscle scar areas are much thicker and more expressed compared with specimens with a thinner shell (Figs. 4I, 4M, 4R and 4T).

Distribution: This species was recorded on the oceanic slope of the Kuril Islands (45°01.202′N, 151°06.008′E–47°14.32′N, 154°42.26′E) at a depth of 4,982–5,766 m, on the abyssal plain adjacent to the Kuril-Kamchatka Trench (39°43.43′N, 147°09.98′E–46°13.99′N, 155°33.10′E) at a depth of 4,860–5,497 m (bottom temperature (6–8 m above bottom) 1.5–1.6 °C, salinity 34.7‰, oxygen 7.71–7.72 ml. l−1) (Brandt et al., 2015), in the Kuril-Kamchatka Trench (44°06.152′N, 151°25.705′E–45°56.821′N, 152°51.185′E) at a depth of 6,068–9,540 m, and in the Japan Trench (36°44′N, 143°19′E–38°38′N, 144°06′E) at a depth of 7,350–7,540 m.

Comparisons: Axinulus krylovae sp. nov. strongly differs from all species of the genus Axinulus in having expressed thickenings of the shell in the adductor muscle scar areas, which are visible on the outer surface of the anterior and posterior parts of the shell as wide radial rays extending from the beaks (Table 3). Due to the shell thickening, the muscle scars are raised to various degrees above the inner shell surface, depending on the thickness of the shell itself. Also, Axinulus krylovae sp. nov. is clearly distinguished from all the other species by a unique sculpture of the prodissoconch (Table 3). Moreover, the new species well differs from almost all species of the genus Axinulus in having a conspicuous radial sculpture of the shell. Judging by photos of the type material and descriptions of species, A. brevis, A. croulensis, and A. philippinensis also have a radial sculpture on the shell surface, but it is less distinct and was described as fine radial lines or a peculiar radial texture (Oliver & Killeen, 2002; Allen, 2015). In contrast to most species of the genus, the new species has a larger shell. Only A. oliveri and A. roseus also have a large shell, but unlike Axinulus krylovae sp. nov., their shells lack the radial sculpture and inner thickenings in the adductor muscle scar areas and are characterized by a well defined escutcheon (Kamenev, 2020). In addition, the above species have extensively lobed lateral pouches.

Table 3:
Main differentiating characters of Axinulus species.
Species Maximum shell length and height (mm) Shell Sculpture Escutcheon Auricle Lunule Ligament Lateral pouch Prodissoconch length (µm) and sculpture References
Axinulus krylovae sp. nov. L = 5.2 H = 5.7 Elongated ovate to rhomboidal; anterodorsal and posterodorsal shell margins convex Closely spaced, commarginal riblets with conspicuous radial ribs and rays and weak, irregular, undulations Absent Absent Weakly defined, as a crest, raised, long, wide Slightly visible externally as a narrow strip between valves Large, no marked lobes 131–158; initial part with tubercle and depression, sculptured numerous, short folds and radial, long folds Present study
Axinulus alatus sp. nov. L = 2.7 H = 3.1 Oval to ovate-polygonal; anterodorsal and posterodorsal shell margins convex Closely spaced, commarginal riblets; micro-sculpture of densely spaced pits Weakly defined, long, narrow Present, distinct Weakly defined, as a weak crest, short, narrow, lanceolate Not visible externally Small, no marked lobes 161–174; initial part with densely spaced, short folds and wrinkles and two series of commarginal, long folds Present study
Axinulus cristatus sp. nov. L = 3.6 H = 3.8 Pyriform; anterodorsal and posterodorsal shell margins convex Closely spaced, commarginal ribs and narrow, closely spaced, radial rays formed by closely spaced, finest, short, concentric wrinkles Absent Absent Weakly defined, with a weak crest, long, wide Not visible externally No data 127; with 24 thin, almost straight folds extending from a long, high crest, located in mid-line of prodissoconch Present study
Axinulus roseus L = 8.7 H = 9.3 Rhomboidal; anterodorsal shell margin concave; posterodorsal shell margin convex or straight Conspicuous, commarginal, narrow ridges with sparse, thin, radial rays from microscopic concentric wrinkles and weak, irregular undulations; microsculpture of densely spaced pits Well defined, very long, narrow Absent Well defined, sunken, long, wide Visible externally Large, extensively lobed 212–215; with an oblique, shallow, wide, elongated sulcus in anterior part Kamenev (2020)
Axinulus oliveri L = 5.7 H = 6.2 Ovate-rhomboidal; anterodorsal shell margin convex or straight; posterodorsal shell margin convex Thin, commarginal ribs and weak, irregular undulations; microsculpture of densely spaced pits Well defined, very long, narrow Present, distinct Weakly defined, with a weak crest, long, wide Not visible externally Large, extensively lobed 191–220; initial part with 9–12 thin, lamellated folds extending from short, plicate ridge, located in mid-line of prodissoconch Kamenev (2020)
Axinulus antarcticus H = 2.9 Subquadrate; anterodorsal shell margin horizontal, straight; posterodorsal shell margin straight Thin, commarginal ribs Weakly defined, long, narrow Present, distinct Weakly defined, short, wide Visible externally Small, no marked lobes 115; sculptured with numerous folds, radiating from a central axis Zelaya (2010)
Axinulus philippinensis L = 3.2 Oblique, angular; anterodorsal shell margin convex or straight; posterodorsal shell margin convex or straight Fine, growth lines and marginal, radial lines Absent Absent No data No data Markedly lobed at margins No data Allen (2015)
Axinulus alleni L = 2.5 Subquadrate; anterodorsal shell margin straight or convex; posterodorsal shell margin convex Very fine, growth lines Well defined, long, narrow Present, distinct No data Visible externally Large, swollen, without lobes No data Carrozza (1981), Payne & Allen (1991)
Axinulus brevis L = 2.70 H = 3.19 Upright oval outline; sometimes pyriform; anterodorsal and posterodorsal shell margin convex Very fine, growth lines and thin, radial riblets Weakly defined, no sunken Absent Weakly defined Visible externally Large, elongate, no marked lobes 135–175 Verrill & Bush (1898), Payne & Allen (1991)
Axinulus croulensis L = 1.8 H = 2.0 Upright oval or almost circular outline; anterodorsal shell margin horizontal, straight; posterodorsal shell margin convex Very fine, commarginal lines and growth stops, mostly glossy with a radial texture Absent Indistinct Absent Visible externally Large, with small projecting lobes along dorsal margin 121–141 Payne & Allen (1991), Oliver & Killeen (2002)
Axinulus subequatorius L = 3.3 H = 3.5 Pyriform; anterodorsal and posterodorsal shell margins convex Fine, ill-defined growth lines Present Absent No data No data With a number of small peripheral lobes No data Payne & Allen (1991)
DOI: 10.7717/peerj.15543/table-3

Note:

L, shell length; H, shell height.

Derivation of name: The new species was named in honor of the well-known malacologist E.M. Krylova, who made a significant contribution to the study of the world fauna of the family Vesicomyiidae and the superfamily Cuspidarioidea and has always provided invaluable help in my research on the deep-sea bivalve fauna of the northern Pacific.

Remarks: Axinulus krylovae sp. nov. has more or less expressed inner thickenings of the shell, depending on the shell thickness, in the area of adductor muscle scars. As a result, the muscle scars are raised above the inner shell surface. The thickened shell area is, as a rule, more pronounced posteriorly than anteriorly. The presence of elevated adductor scars is a characteristic and main distinguishing feature of species of the genus Genaxinus Iredale, 1930 from other genera of the family Thyasiridae (Hedley, 1907; Iredale, 1930; Oliver & Killeen, 2002; Oliver & Levin, 2006; Oliver, 2015). Nevertheless, I think the new species should be assigned to the genus Axinulus but not to Genaxinus. In species of Genaxinus, only muscle scars themselves are raised. The new species has the thickened anterior and posterior parts of shell, which are larger in area than the muscle scars proper, and these thickened areas bear muscle scars. The degree of inner shell thickening significantly varies, thus influencing the degree of elevation of muscle scars above the inner shell surface. Unlike the species described herein, in species of the genus Genaxinus, muscle scars bear prominent concentric growth marks that were formed during migration of the muscle during growth. In the Genaxinus species, the muscle scars are white color, project from the inner shell surface, and are well visible through the shell from outside (Payne & Allen, 1991; Oliver & Killeen, 2002).

The thickenings of the shell in muscle scar areas analogous to those of Axinulus krylovae sp. nov. are also characteristic of other species of the Thyasiridae (e.g., A. croulinensis, A. alleni, Mendicula ultima (Payne & Allen, 1991)) (Payne & Allen, 1991; Oliver & Killeen, 2002). In these species, inner shell thickening is also more expressed in the posterior area of the shell. Most probably, thorough studies of small thyasirids using scanning electron microscopy will reveal analogous inner shell thickenings in some other species as well.

The new species described here has a wide geographic and vertical distribution in the northwestern Pacific Ocean. It was found in large numbers in many samples from this vast region. This allowed a study of both the age and individual variability of its shell morphology. On the whole, the shell shape and proportions vary greatly, irrespective of the sampling area and depth. Specimens found in different areas and at different depths were of different size and had both the rounded and dorsoventrally elongated shell shape. A thorough examination of the shell morphology of specimens from various parts of the species range showed that they have the same main morphological characters (shell sculpture, hinge plate morphology, inner shell thickenings in muscle scar areas, prodissoconch sculpture). I found no unique morphological and anatomical features characteristic of a group of specimens. Moreover, specimens with a transitional shape of the shell between elongated oval and almost rounded were found in samples. Therefore, I think that specimens with different shell shape belong to the same species. It should be noted that many species of the Thyasiridae, among them species of Axinulus, exhibit a large age and individual variability of the shell shape and proportions (Payne & Allen, 1991; Oliver & Killeen, 2002; Allen, 2015; Kamenev, 2020).

Axinulus krylovae sp. nov. was found in the Pacific Ocean in a wide depth range (about 5,000 m). An earlier study recorded three species of bivalves with a vertical distribution range of 4,000 to 7,000 m among the hadal fauna of the Kuril-Kamchatka Trench. In addition, a number of eurybathic species from various taxonomic groups have a vertical distribution range greater than 5,000–7,000 m (Kamenev, 2019). By all appearances, A. krylovae sp. nov. also belongs to the group of eurybathic species inhabiting a wide depth range in the abyssal and hadal zones of the Pacific Ocean.

Axinulus alatus sp. nov.

(Figs. 69, Tables 4 and 5)

Axinulus alatus sp. nov.

Figure 6: Axinulus alatus sp. nov.

(A–D) Holotype ( MIMB 45893), exterior, dorsal, and oblique dorsal views of both valves, shell length 2.4 mm. (E and F) Interior view and hinge plate of right valve, valve length 2.7 mm. (G) Paratype (MIMB 45894), exterior view of right valve, shell length 2.7 mm. (H and I) Paratype (ZMF 372430), exterior view of right valve and dorsal view of both valves, shell length 2.3 mm. (J–M) Variability of shell shape, exterior view of right valves: (J) Shell length 1.7 mm; (K) Shell length 2.5 mm ; (L) Shell length 1.5 mm; (M) Shell length 2.1 mm. Abbreviation: R, radial, whitish ray. Scale bar: F = 500 µm.
Scanning electron micrographs of Axinulus alatus sp. nov.

Figure 7: Scanning electron micrographs of Axinulus alatus sp. nov.

(A) Dorsal view of both valves. (B and C) Escutcheon. (D) Lunule. (E) Exterior view of left valve. (F) Sculpture of beak region. (G) Posterodorsal margin of left valve with auricle. (H) Sculpture of ventral shell part. (I and J) Sculpture of central shell part. (K) Interior view of right valve. (L) Hinge plate and ligamental groove of right valve. (M and N) Ventral view of hinge plate and ligamental groove of right valve. (O) Interior view of left valve. (P) Hinge plate and ligamental groove of left valve. (Q and R) Ventral view of hinge plate and ligamental groove of left valve. Scale bars: A–H, K–R = 100 µm ; I, J = 10 µm.
Scanning electron micrographs of prodissoconchs of Axinulus alatus sp. nov.

Figure 8: Scanning electron micrographs of prodissoconchs of Axinulus alatus sp. nov.

(A–F) Prodissoconchs of specimens from oceanic slope of the Kuril Islands, depth 3,432 m. (G and H) Prodissoconchs of specimens from the Bering Sea, depth 3,978 m. Scale bars: A–H = 50 µm.
Axinulus alatus sp. nov.

Figure 9: Axinulus alatus sp. nov.

(A) Exhalant apertures. (B) Gross anatomy after removal of right valve and mantle. (C) Gross anatomy after further removal of right ctenidium. (D) Gross anatomy after further removal of right lateral body pouch. (E) Digestive system. (F) Gross anatomy after removal of right valve, mantle, right ctenidium, and bulbous portion of foot. (G) Gross anatomy after further removal of right lateral body pouch and mantle edge. (H) Digestive system. Abbreviations: AA, anterior adductor muscle; APR, anterior pedal retractor muscle; EA, exhalant aperture; F, foot; HG, hind gut; ID, inner demibranch; K, kidney; L, ligament; LBP, lateral body pouch; LP, labial palps; ME, mantle edge; MG, mid gut; NP, neck of lateral body pouch; PA, posterior adductor muscle; PPR, posterior pedal retractor muscle; S, stomach. Scale bars: A–H = 500 µm.
Table 4:
Additional material of Axinulus alatus sp. nov. examined in the present study.
Ship, cruise no. Station Date Start End Depth (m) Gear N Depository
Latitude °N Longitude °E Latitude °N Longitude °E
Abyssal slope of the Kuril Islands, Pacific Ocean
Akademik M.A.
Lavrentyev 71
9-1 27.07.2015 46°16.082′ 152°02.060′ 3,432 GKG 10 MIMB 45895
9-6 26.07.2015 46°16.103′ 152°02.004′ 46°16.132′ 152°03.036′ 3,436–3,377 EBS 2 MIMB 45896
9-7 26.07.2015 46°16.129′ 152°02.440′ 46°16.070′ 152°03.324′ 3,409–3,377 EBS 1 MIMB 45897
Commander Basin, Bering Sea
Akademik Mstislav Keldysh 22 2309 31.07.1990 55°13.2′ 167°29.07′ 55°12′ 167°26.7′ 3,957–3,978 ST 73 IORAS BIV00989
2316 05.08.1990 55°36.1′ 167°23.04′ 55°35′ 167°24.5′ 4,294–4,200 ST 1 IORAS BIV00990
Kodiak Island, Gulf of Alaska, Pacific Ocean
Vityaz 45 6095 08.05.1969 57°37.7′ 148°34.5′ W 3,200 OG 2 IORAS BIV00991
DOI: 10.7717/peerj.15543/table-4

Note:

OG, Okean grab (0.25 m2); GKG, giant box corer (0.25 m2); EBS, epibenthic sledge; ST, Sigsbee trawl; N, number of live specimens.

Table 5:
Axinulus alatus sp. nov.
Shell measurements (mm), indices and summary statistics of indices.
Depository L H A W H/L A/L W/L
Paratype MIMB 45894 2.7 2.8 1.1 1.7 1.037 0.407 0.630
Paratype IORAS BIV00988 2.6 2.9 1.0 1.7 1.115 0.385 0.654
Paratype IORAS BIV00988 2.5 2.8 1.0 1.7 1.120 0.400 0.680
Paratype IORAS BIV00988 2.6 2.8 1.0 1.6 1.077 0.385 0.615
Paratype IORAS BIV00988 2.5 2.8 1.0 1.6 1.120 0.400 0.640
Paratype IORAS BIV00988 2.5 2.6 1.0 1.5 1.040 0.400 0.600
Holotype MIMB 45893 2.4 2.6 1.0 1.5 1.083 0.417 0.625
Paratype SMF 372430 2.3 2.6 0.9 1.4 1.130 0.391 0.609
IORAS BIV00989 2.4 2.6 0.9 1.5 1.083 0.375 0.625
IORAS BIV00989 2.3 2.5 0.8 1.4 1.087 0.348 0.609
IORAS BIV00989 2.2 2.4 0.8 1.4 1.091 0.364 0.636
IORAS BIV00989 2.2 2.4 0.9 1.3 1.091 0.409 0.591
IORAS BIV00989 2.2 2.4 0.8 1.3 1.091 0.364 0.591
IORAS BIV00989 2.1 2.3 0.7 1.3 1.095 0.333 0.619
IORAS BIV00989 2.1 2.3 0.8 1.3 1.095 0.381 0.619
IORAS BIV00989 2.1 2.5 0.7 1.2 1.190 0.333 0.571
IORAS BIV00989 2.1 2.3 0.7 1.3 1.095 0.333 0.619
IORAS BIV00989 2.0 2.4 0.7 1.4 1.200 0.350 0.700
IORAS BIV00989 2.0 2.2 0.9 1.3 1.100 0.450 0.650
IORAS BIV00989 2.0 2.3 0.7 1.3 1.150 0.350 0.650
IORAS BIV00989 2.0 2.2 0.6 1.3 1.100 0.300 0.650
IORAS BIV00989 2.0 2.1 0.8 1.2 1.050 0.400 0.600
IORAS BIV00989 1.9 2.2 0.6 1.2 1.158 0.316 0.632
IORAS BIV00989 1.9 2.0 0.7 1.2 1.053 0.368 0.632
IORAS BIV00989 1.9 2.1 0.6 1.2 1.105 0.316 0.632
IORAS BIV00989 1.9 2.1 0.8 1.1 1.105 0.421 0.579
IORAS BIV00989 1.9 2.1 0.7 1.1 1.105 0.368 0.579
IORAS BIV00989 1.9 2.1 0.8 1.1 1.105 0.421 0.579
IORAS BIV00989 1.8 2.0 0.7 1.1 1.111 0.389 0.611
IORAS BIV00989 1.7 1.9 0.5 1.0 1.118 0.294 0.588
IORAS BIV00989 1.6 1.8 0.5 0.9 1.125 0.313 0.563
IORAS BIV00989 1.5 1.7 0.5 0.9 1.133 0.333 0.600
Statistics L H A W H/L A/L W/L
Mean 1.105 0.369 0.618
SE 0.026 0.033 0.024
SD 0.037 0.039 0.031
Min 1.037 0.294 0.563
Max 1.200 0.450 0.700
DOI: 10.7717/peerj.15543/table-5

Note:

L, shell length; H, height; W, width; A, anterior end length.

Axinulus sp.: Kamenev, 2018a, p. 234.

urn:lsid:zoobank.org:act:0BBDAC37-2258-4C8B-89E4-DECF7402C58E

Type material and locality: Holotype (MIMB 45893), slope of the Kuril Islands, Pacific Ocean (46°16.082′N, 152°02.060′E), 3,432 m, boxcorer, Coll. G.M. Kamenev, 27-VII-2015 (RV Akademik M.A. Lavrentyev, cruise no. 71, stn. 9-1); paratype (SMF 372430), from holotype locality; paratypes (5) (IORAS BIV00988) and paratype (MIMB 45894), Commander Basin, Bering Sea (55°13.2′N, 167°29.07′E–55°12′N, 167°26.7′E), 3,957–3,978 m, Sigsbee trawl, Coll. S.V. Galkin, 31-VII-1990 (RV Akademik Mstislav Keldysh, cruise no. 22, stn. 2309).

Other material examined: 89 live specimens (Table 4).

Diagnosis: Shell small (to 2.9 mm in length), oval to ovate-polygonal, slightly drawn out anterior, with two slightly curved, radiating, whitish, elongate-triangular, opaque rays extend from beaks to anteroventral and posteroventral margins. Sculpture of closely spaced, commarginal riblets. Micro-sculpture of densely spaced pits. Posterior folds and sulcus absent. Escutcheon long, narrow, shallow. Auricle long, low. Lunule as a weak crest, slightly raised, short, lanceolate. Ligament sunken, not visible externally, short. Prodissoconch medium in size (length 161–174 µm); initial part with small area of densely spaced, short, curved folds and wrinkles and two series of commarginal, long, thin folds extending as wings from this area. Adductor muscle scars distinct, elongated triangular in outline; posterior scar raised above inner shell surface due to thickening of shell. Lateral body pouches small, simple, without projecting lobes.

Description. Shell small (to 2.9 mm in length and 3.1 mm in height), inflated (W/L = 0.618 ± 0.024), higher than long (H/L = 1.105 ± 0.026), slightly drawn out anteriorly, median area divided by a weak change in angulation; oval to ovate-polygonal, sometimes slightly pyriform, subequilateral, white, thin, translucent, with two slightly curved, radiating, whitish, elongate-triangular, opaque rays extending from beaks to anteroventral and posteroventral margins, respectively, more marked in posterior shell area, formed by internal thickening of shell associated with anterior and posterior adductor muscles; patches of silty deposit adhering to anterior and posterior shell margins (Fig. 6 and Table 5). Periostracum very thin, colorless, translucent, adherent. Dissoconch sculptured with thin, closely spaced, commarginal riblets. Micro-sculpture of small, densely spaced pits. Beaks small, prosogyrate, curved slightly inwards, anterior to midline (A/L = 0.369 ± 0.033) (Fig. 7 and Table 5). Anterodorsal shell margin long, convex, steeply sloping from beaks, forming a rounded angle at transition to anterior margin. Anterior shell margin curved, smoothly transitioning to ventral margin. Ventral margin strongly curved, slightly angulate. Posterodorsal shell margin long, convex, steeply sloping from beaks, forming a broadly rounded angle at transition to posterior margin. Posterior shell margin slightly curved, smoothly transitioning to ventral margin. Posterior folds absent but posterior shell area a little flattened. Escutcheon long, almost as long as entire length of posterodorsal shell margin, narrow, shallow, demarcated by low ridges. Auricle long, almost as long as entire length of escutcheon, weak, low, only slightly projecting (Figs. 7B, 7C and 7G). Lunule as a weak crest, slightly raised, short, about half length of anterodorsal shell margin, narrow, lanceolate, weakly defined by low and thin ribs (Fig. 7D). Ligament opisthodetic, sunken, not visible externally, short, about one third of entire length of the escutcheon, thick, almost straight, lying in deep, wide groove in hinge plate (Figs. 7L7N, 7P7R). Prodissoconch medium in size (length 161–174 µm), distinctly separated from dissoconch, ovate in outline. Initial part of prodissoconch with densely spaced, short, curved folds and wrinkles interspersed with pits, forming a rounded zone. This zone giving rise to two series of commarginal, long, thin, low folds (about 20 folds in each series), extending as wings in different directions; remaining surface of prodissoconch smooth (Fig. 8). Hinge plate thin, edentulous, with almost straight ligamental groove (Figs. 7L and 7P). Adductor muscle scars distinct, long, elongated triangular in outline, extending into umbonal cavity, bearing thin, radial striation. Posterior adductor scar narrow, straight, raised above inner shell surface due to thickening of shell in this area. Anterior adductor scar wider than posterior one, curved, not raised above inner shell surface (Figs. 7K, 7M, 7O and 7Q).

Anatomy: Mantle thin; margins thickened, unfused except long posterior suture with two exhalant apertures below the posterior adductor (Fig. 9A). Anterior adductor muscle elongated, two times longer than posterior adductor, slightly curved, dorsal part narrower than ventral part. Posterior adductor muscle small, oval (Figs. 9B and 9C). Ctenidium thin, narrow, consisting of a single inner demibranch with fully reflected filaments (up to 30 filaments in largest specimen 2.9 mm in shell length). Demibranch not covering lateral body pouches and consisting of both ascending and descending lamellae; ascending lamellae slightly shorter than descending lamellae. Labial palps small, as extensions of the short (about 300 µm), oral grooves (Fig. 9B). Lateral body pouches small (Figs. 9C and 9F), dorsoventrally elongated, outline oval, with undulated margins, without projecting lobes; each pouch connecting to body by a wide neck (Fig. 9D). Kidneys large, dorsoventrally elongated along posterodorsal shell margin, without granules (Figs. 7C7G). Alimentary system with short oesoghagus leading to an elongate stomach; combined style sac and midgut strongly curved, forming a deep and narrow loop between neck of lateral pouches and kidney; hind gut forming an anterior, deep, and wide loop dorsal to style sac, running posteriorly dorsal to kidney and posterior adductor muscle, opening at ventral side of posterior adductor muscle (Figs. 7E and 7H). Foot long, vermiform, distally bulbous, with a muscular ring at the junction with the visceral mass. Bulbous portion of foot not divided into two distinct parts; surface with densely spaced papillae; heel absent. Anterior and posterior pedal retractors short, narrow (Figs. 7B, 7C and 7F).

Variability: The shell shape and proportions vary little among different-sized specimens (Figs. 6G6M and Table 5). The shell shape is oval to ovate. Some specimens have a more angular and relatively shortened shell. In small specimens (less than 2 mm in shell length), the shell is more elongated dorsoventrally, with a more anteriorly drown-out ventral margin.

Distribution: This species was recorded on the oceanic slope of the Kuril Islands (46°16.082′N, 152°02.060′E–46°16.129′N, 152°03.324′E) at a depth of 3,377–3,436 m, at the bottom of Commander Basin (Bering Sea) (55°12.7′N, 167°26.7′E–55°36.1′N, 167°23.04′E) at a depth of 3,957–4,294 m, and off Kodiak Island (Gulf of Alaska, Pacific Ocean) (57°37.7′N, 148°34.5′W) at a depth of 3,200 m.

Comparisons: Axinulus alatus sp. nov. differs from all species of the genus Axinulus in having a unique sculpture of the prodissoconch (Table 3). In terms of the shape, proportions, and size of the shell, A. alatus sp. nov. is closest to A. brevis, A. croulensis, A. antarcticus, and A. subequatorius. However, unlike A. brevis and A. croulensis, the new species described here lacks radial sculpture of the shell and has a well-defined, long auricle, and a deep sunken ligament invisible externally. Apart from the differences in prodissoconch sculpture, A. alatus sp. nov. is distinguished from A. antarcticus by having a long lanceolate lunule and a deep sunken ligament invisible externally. Axinulus alatus sp. nov. differs from A. subequatorius in lacking projecting lobes of lateral body pouches and in having two posterior inhalant apertures but not one (Payne & Allen, 1991). Axinulus alatus sp. nov. is similar in shell shape and proportions to some species of the genus Adontorhina Berry, 1947, but it is readily distinguished from them by the absence of irregular, minute granules on the hinge plate (Scott, 1986; Kamenev, 1996, 2013; Coan, Scott & Bernard, 2000; Güller & Zelaya, 2011; Coan & Valentich-Scott, 2012; Valentich-Scott, Coan & Zelaya, 2020).

Derivation of name: The species epithet meaning “winged” indicates the similarity of two series of long, commarginal folds of the prodissoconch to the extended wings of a flying bird.

Remarks: Axinulus alatus sp. nov. has a wide range in the northern Pacific Ocean and was found off the Asian and American continents in the lower abyssal zone in the depth range of 3,000–5,000 m. The species was not found among extensive material of bivalves collected by the numerous deep-sea expeditions at depths greater than 5,000 m at the abyssal plain adjacent to the Kuril-Kamchatka and Japan trenches (Okutani, 1974, 2000; Okutani & Fujikura, 2002; Okutani & Kawamura, 2002; Kamenev, 2015, 2018a), as well as in the hadal zone of the Aleutian, Kuril-Kamchatka, and Japan trenches (Okutani, 1974, 2003; Okutani, Fujikura & Kojima, 1999; Fujikura et al., 1999, 2002; Okutani & Fujiwara, 2005; Kamenev, 2019, 2020, 2023). In addition, this species was not recorded for the benthic fauna of the Kuril Basin (the Sea of Okhotsk) a little less than 3,300 m deep (Kamenev, 2018a), though it was found on the oceanic slope of the Kuril Islands opposite the deepest Bussol Strait connecting the Sea of Okhotsk and the Pacific Ocean. The deep-sea ecosystem of the Kuril Basin is characterized by low oxygen concentrations near the bottom, largely influencing the composition and quantitative distribution of benthic fauna (Kamenev et al., 2022). It is possible that oxygen deficiency exerts a negative influence, not allowing the species to live in the Kuril Basin. In the Commander Basin (Bering Sea), in contrast to the Kuril Basin, A. alatus sp. nov. forms high-density populations. Probably, A. alatus sp. nov. is a low abyssal species, preferring depths of 3,000–5,000 m.

Axinulus cristatus sp. nov.

(Fig. 10)

Scanning electron micrographs of the holotype of Axinulus cristatus sp. nov. (MIMB 45892).

Figure 10: Scanning electron micrographs of the holotype of Axinulus cristatus sp. nov. (MIMB 45892).

(A) Exterior view of right valve. (B–D) Sculpture of ventral shell part. (E) Interior view of left valve. (F) Ventral view of left valve. (G) Hinge plate and ligamental groove of left valve. (H and I) Spoon-shaped tubercle under beak in left valve. (J–M) Prodissoconch. Scale bars: A, E, F = 500 µm; B, G, H = 100 µm; C, I, J–M = 50 µm; D = 10 µm.

urn:lsid:zoobank.org:act:BFDA7E99-6561-4597-B5BE-5037F42815B0

Type material and locality: Holotype (MIMB 45892), Kuril-Kamchatka Trench, Pacific Ocean (45°56.821′N, 152°51.185′E – 45°56.834′N, 152°50.943′E), 6,168–6,164 m, epibenthic sledge, Coll. A. Brandt, 27-VIII-2016 (RV Sonne, cruise no. 250, stn. 30).

Diagnosis: Shell medium in size (to 3.6 mm in length), pyriform. Sculpture of closely spaced, commarginal ribs and narrow, radial rays consisting of fin, short, concentric wrinkles. Posterior folds and sulcus absent. Escutcheon and auricle absent. Lunule as a weak crest, raised, long, wide, weakly defined. Ligament sunken, long. Prodissoconch small (length 127 µm), with 24 thin, almost straight folds of different length, extending from high crest, located in mid-line of prodissoconch. Adductor muscle scars distinct, elongated triangular in outline, not raised above inner surface of shell. Inner shell surface with distinct, thin, radial striae.

Description: Shell medium in size (to 3.6 mm in length and 3.8 mm in height), pyriform, equivalve, subequilateral, white, thick, inflated, slightly higher than long (H/L = 1.043) (Fig. 10). Periostracum thin, colorless, translucent, adherent. Dissoconch sculptured with thin, closely spaced, commarginal ribs and narrow, closely spaced, radial rays of varying width, formed by closely spaced, finest, short, concentric wrinkles; radial rays becoming widest and prominent along shell margins (Figs. 10B10D). Beaks small, raised, prosogyrate, anterior to midline (A/L = 0.312). Anterodorsal shell margin convex, steeply sloping from beaks, smoothly transitioning to anterior margin. Anterior margin curved, smoothly transitioning to ventral margin. Ventral margin gently curved. Posterodorsal margin long, slightly convex, steeply sloping from beaks, descending to mid-point of shell, forming a rounded angle at transition to slightly curved posterior margin. Posterior folds and sulcus absent. Escutcheon and auricle absent. Lunule as a weak crest, raised, long, wide, weakly defined, demarcated by weak, long, rounded ridges along entire anterodorsal shell margin. Ligament opisthodetic, sunken, thick, evenly curved, long, about half the length of posterodorsal shell margin, lying in deep, curved groove at surface of hinge plate (Figs. 10E10G). Prodissoconch small (length 127 µm), distinct, oval in outline, convex, with 24 thin, almost straight folds of different length, sometimes bifurcated at end, extending from a long, high crest, located in mid-line of prodissoconch; remaining surface of prodissoconch smooth (Figs. 10J10M). Hinge plate thickened, with a distinct, flattened, spoon-shaped tubercle under beak in left valve and a long ligamental groove (Figs. 10G10I). Adductor muscle scars distinct, long, outline elongated triangular, extending into umbonal cavity, bearing thin, indistinct, radial striation, not raised above shell surface. Posterior adductor scar narrow, straight. Anterior adductor scar about two times wider than posterior and curved. Inner shell surface with distinct, thin, radial lines, corresponding to radial sculpture of external shell surface (Figs. 10E and 10F).

Distribution: This species is known only from the holotype locality in the Kuril-Kamchatka Trench at a depth of 6,168–6,164 m.

Comparisons: Axinulus cristatus sp. nov. is readily distinguished from all species of the genus Axinulus in having a radial sculpture in the form of rays consisting of closely spaced, short, fin, wrinkles and distinct radial lines on the inner shell surface, as well as by its unique sculpture of the prodissococh (Table 3). In addition, the new species differs from other species of the genus Axinulus in having a distinct spoon-shaped tubercle on the hinge plate in the left valve. However, the unusual shape of the tubercle on the hinge plate may be an individual abnormality of the examined specimen rather than being a specific character.

Derivation of name: The species name means “crested” and refers to a high crest on the surface of the prodissoconch.

Remarks: Unfortunately, only a single specimen of A. cristatus sp. nov. was found after examining all the material collected by many expeditions in the northwestern Pacific Ocean. Therefore, the gross anatomy of the body of this species is not studied, because the body has partially dissolved during preparation of the specimen for examination in a scanning microscope. In the specimen described here, the gill had only one demibranch; therefore, I assigned the species to the genus Axinulus. Since the shell of the species has a number of unique morphological characters that readily separate it from the congeners, I described it as new to science, despite the minimal material at my disposal. Due to the difficulty of collecting bottom animals from great depths, many deep-sea species are often represented by a single specimen in the benthic fauna collections made by different deep-sea expeditions (Kamenev, 2015, 2019) and are thus described from merely 1–2 specimens (e.g., Knudsen, 1970; Payne & Allen, 1991).

Discussion

Until recently, the genus Axinulus was represented only by six species (Zelaya, 2010; Allen, 2015; Oliver, 2015; Kamenev, 2020). Of these, five species were found in the Atlantic Ocean (Payne & Allen, 1991; Allen, 2008; Zelaya, 2010), while in the Pacific Ocean only one species of the genus, A. philippinensis, was recorded and described from the bottom of the Philippine Trench (Allen, 2015). In recent years, a study of the extensive material of bivalves collected by many deep-sea expeditions mainly in the northwestern Pacific Ocean, another five species of the genus Axinulus were recorded for the Pacific Ocean (Kamenev, 2020). Thus, the number of species in the genus has increased almost twice, and at present the fauna of Axinulus in the Pacific Ocean has more species, compared to the Atlantic Ocean.

Out of all the species found in the Atlantic Ocean, only A. subequatorius was recorded exclusively in the abyssal zone at depths of more than 3,000 m. The other species have a wider vertical distribution range and were recorded in the subtidal and bathyal zones at depths less than 3,000 m. In contrast to Atlantic species, all Pacific species of the genus Axinulus were found exclusively at depths greater than 3,000 m. Moreover, almost all of them, except A. alatus sp. nov., were recorded in the hadal zone of different oceanic trenches at depths of more than 6,000 m (Allen, 2015; Kamenev, 2020). Thus, unlike the Atlantic Ocean, species of the genus Axinulus are typical members of the abyssal and hadal benthic fauna in the northern Pacific Ocean. In addition, the species A. roseus, A. krylovae sp. nov., A. philippinensis, and A. alatus sp. nov. were found in large numbers in samples collected from the floor of the deepest basins of the Kuril-Kamchatka, Japan, and Philippine trenches, and the Bering Sea, respectively (Allen, 2015; Kamenev, 2020; Kamenev et al., 2022), where they probably play a substantial role in the functioning of benthic deep-sea ecosystems in these northwestern Pacific regions.

Presumably, species of the genus Axinulus were found in another seven oceanic trenches, apart from the Kuril-Kamchatka, Japan, and Philippine trenches (Belyaev, 1989). Therefore, further study will most likely increase the species richness of the genus Axinulus.

Unlike many species of the genus Thyasira, many species of the genus Axinulus have a uniquely sculptured prodissoconch, which is one of the important diagnostic characters of thyasirids (Zelaya, 2009, 2010; Kamenev, 2020, 2023). The prodissoconch sculpture of A. brevis, A. alleni, A. subequatorius, and A. philippinensis has not been reported. Likewise, the prodissoconch of A. croulensis, which is smooth and lacks sculpture, was not examined with a scanning microscope (Oliver & Killeen, 2002). Perhaps, the prodissoconch of all the above species has an external sculpture that can be distinguished only at high magnification using scanning microscopy. It cannot be ruled out that further examination of the prodissoconch of these species will reveal more morphological features, allowing a more accurate diagnosis of largely morphologically similar species of the genus Axinulus.

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