Interval training has a beneficial effect for treating the cardiovascular disease. However, it is not clarify the effects of different exercise intensity and duration on arterial function. In the present study, we evaluated the effect of acute short-term high and moderate-intensity interval exercise on pulse wave velocity. Thirteen healthy men were randomly assigned to perform both acute interval exercise (IE; cycling for 24.6 min at 80%VO₂max and 50%VO₂max) and acute continuous moderate-intensity exercise (CME; cycling for 30 min at 50%VO₂max). The IE and CME protocols were designed such that the exercises resulted in the same workload during each session. The brachial systolic and diastolic blood pressure (SBP and DBP), and brachial to ankle pulse wave velocity (baPWV) were obtained in the supine position using an automatic pulse wave form analyzer at pre- and post-exercise. In IE, baPWV at post-exercise decreased significantly compared with pre-exercise value (1181.9±119.2 cm・sec^-1 vs. 1108.4±109.4 cm・sec^-1, p<0.01). In CME, however, it was not significantly different between pre- and post-exercise values (1173.5±137.1 cm・sec^-1 vs. 1164.8±96.0 cm・sec^-1, p=ns). No significant differences in SBP and DBP were found both protocols. These results suggest that acute short-term high and moderate-intensity interval exercise was more effective in reducing baPWV compared with acute continuous exercise.