Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital
- Published
- Accepted
- Subject Areas
- Neurology, Oncology, Translational Medicine
- Keywords
- Glioma, Biobank, Quality evaluation
- Copyright
- © 2017 Kong et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Establishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital. PeerJ Preprints 5:e3478v1 https://doi.org/10.7287/peerj.preprints.3478v1
Abstract
Background: We established a glioma biobank at Beijing Tiantan Hospital in November, 2010.Specialized staffs have been trained to collect, store and manage the biobank in accordance with standard operating procedures.
Methods: One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination, housekeeping gene expression and protein integrity determination.
Results: The majority of samples (95%) remain high RNA quality for further analysis with RIN≥6. All samples remain high DNA and protein quality without significant degradation.
Conclusion: Storage conditions of our biobank are suitable for long-term (at least 5 years) sample preservation with high molecular quality.
Author Comment
This is a submission to PeerJ for review.