Population structure and phenotypic variation of Sclerotinia sclerotiorum from dry bean (Phaseolus vulgaris) in the United States
- Published
- Accepted
- Subject Areas
- Agricultural Science, Genetics, Mycology
- Keywords
- clonality, microsatellite, network analysis, plant breeding, plant pathogen, white mold, fungal genetics, mycelial compatibility group, population genetics, Phaseolus vulgaris
- Copyright
- © 2017 Kamvar et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Population structure and phenotypic variation of Sclerotinia sclerotiorum from dry bean (Phaseolus vulgaris) in the United States. PeerJ Preprints 5:e3311v2 https://doi.org/10.7287/peerj.preprints.3311v2
Abstract
The ascomycete pathogen Sclerotinia sclerotiorum is a necrotrophic pathogen on over 400 known host plants, and is the causal agent of white mold on dry bean. Currently, there are no known cultivars of dry bean with complete resistance to white mold. For more than 20 years, bean breeders have been using white mold screening nurseries with natural populations of S. sclerotiorum to screen new cultivars for resistance. It is thus important to know if the genetic diversity in populations of S. sclerotiorum within these nurseries a) reflect the genetic diversity of the populations in the surrounding region and b) are stable over time. Furthermore, previous studies have investigated the correlation between mycelial compatibility groups (MCG) and multilocus haplotypes (MLH), but none have formally tested these patterns. We genotyped 366 isolates of S. sclerotiorum from producer fields and white mold screening nurseries surveyed over 10 years in 2003–2012 representing 11 states in the United States of America, Australia, France, and Mexico at 11 microsatellite loci resulting in 165 MLHs. Populations were loosely structured over space and time based on analysis of molecular variance and discriminant analysis of principal components, but not by cultivar, aggressiveness, or field source. Of all the regions tested, only Mexico (n=18) shared no MLHs with any other region. Using a bipartite network-based approach, we found no evidence that the MCGs accurately represent MLHs. Our study suggests that breeders should continue to test dry bean lines in several white mold screening nurseries across the US to account for both the phenotypic and genotypic variation that exists across regions.
Author Comment
This version is revised with minor revisions from peer review.
Permanent link to data and code: https://osf.io/k8wtm
Github Repository: https://github.com/everhartlab/sclerotinia-366
Docker Repository: https://hub.docker.com/r/everhartlab/sclerotinia-366