Immunogenicity evaluation of MS2 phage-mediated chimeric nanoparticle displaying an immunodominant B cell epitope of foot-and-mouth disease virus
- Published
- Accepted
- Subject Areas
- Bioengineering, Biotechnology, Immunology
- Keywords
- Keywords: Foot-and-mouth disease (FMD), MS2 Bacteriophage, chimeric nanoparticles (CNPs), G-H Loop
- Copyright
- © 2018 Wang et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Immunogenicity evaluation of MS2 phage-mediated chimeric nanoparticle displaying an immunodominant B cell epitope of foot-and-mouth disease virus. PeerJ Preprints 6:e26448v1 https://doi.org/10.7287/peerj.preprints.26448v1
Abstract
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals that has caused tremendous economic losses worldwide. In this study, we designed a chimeric nanoparticles vaccine with the predominant epitope of FMDV (VP1 131–160) displayed on the top of the coat protein (CP) of MS2 phage. The recombinant protein was expressed in E. coli and can self-assembled into chimeric nanoparticles (CNPs) with diameter 20-25nm. A tandem repeat peptide epitopes (VP1 131–160) (TRE) was prepared as control. Mice immunized with CNPs and TRE respectively and immunogenicity evaluated show that CNPs stimulated equivalent specific antibody levels to commercialized synthetic peptide vaccines (PepVac), but was significantly higher than TRE groups. Moreover, results from specific IFN-γ responses and lymphocyte proliferation test indicated that CNPs immunized mice exhibited significantly enhanced cellular immune response. These studies suggested that the CNPs constructed in current study could be a potential alternative vaccine in future FMDV control.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Raw data of ELISA to detect specific antibodies
Mice were immunized with PepVac, CNPs, TRE and PBS respectively. Mice sera were collected every week after immunization and used to detect the specific antibodies against inactivated FMDV by ELISA.
Raw data of lymphocyte proliferation assay
The T-lymphocyte proliferation of mice immunized with synthetic peptide vaccine, CNPs, TRE and PBS on 56 dpv. SI: OD450nm value of culture with FMDV stimulation/OD450nm value of culture without stimulation.
Raw data of cytokine levels
Splenic lymphocytes culture supernatants used in the proliferation assay were collected for evaluating IL-2, IL-4 and IFN-γ concentration. The assay were performed by the commercially available ELISA kit