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Whole-brain ex-vivo quantitative MRI of the cuprizone mouse

, , , , , , ,
1 Department of Neuroimaging, IOPPN, King's College London, London, United Kingdom
2 Centre for Functional MRI of the Brain, University of Oxford, Oxford, Oxfordshire, United Kingdom
3 Synaptive Medical, Toronto, ON, Canada
4 Cells and Behaviour Unit, Department of Basic and Clinical Neuroscience, IOPPN, King's College London, London, United Kingdom
5 NatBrainLab, Department of Basic and Clinical Neuroscience, IOPPN, King's College London, London, United Kingdom
DOI
10.7287/peerj.preprints.2323v1
Published
Accepted
Subject Areas
Neuroscience, Radiology and Medical Imaging, Histology
Keywords
cuprizone, myelin, quantitative imaging, MRI
Copyright
© 2016 Wood et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Wood TC, Simmons C, Hurley SA, Vernon AC, Torres J, Dell'Acqua F, Williams SCR, Cash D. 2016. Whole-brain ex-vivo quantitative MRI of the cuprizone mouse. PeerJ Preprints 4:e2323v1

Abstract

Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component DESPOT, Diffusion Tensor Imaging and Tensor Based Morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. However the presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model.

Author Comment

This is a submission to PeerJ for review.

Additional Information

Competing Interests

The authors declare that they have no competing interests. Samuel A Hurley is an employee of Synaptive Medical, Toronto, ON, Canada.

Author Contributions

Tobias C Wood conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Camilla Simmons performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Samuel A Hurley contributed reagents/materials/analysis tools, reviewed drafts of the paper, provided the DESPOT sequences for the Agilent scanner.

Anthony C Vernon conceived and designed the experiments, reviewed drafts of the paper.

Joel Torres analyzed the data.

Flavio Dell'Acqua reviewed drafts of the paper, assisted with the diffusion sequence for the Agilent scanner.

Steve C R Williams conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Diana Cash conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All experiments were performed under approval of the local KCL ethics committee and the UK Animals (Scientific Procedures) Act 1986, according to the Home Office Project license no 70/8480 (held by Diana Cash).

Data Deposition

The following information was supplied regarding data availability:

1) Code - http://github.com/spinicist/QUIT -

2) Quantitative maps & stats images - https://figshare.com/articles/Whole-Brain_Ex-Vivo_Quantitative_MRI_of_the_Cuprizone_Mouse/3495848

Funding

The study received funding from the MRC (grant code G0800298). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Whole-brain ex-vivo quantitative MRI of the cuprizone mouse
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