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2014 Vol.32, Issue 5 Preview Page
Antioxidant Enzymes and Antimicrobial Activities in Sponge Gourds

수세미 오이의 항산화 효소 및 항균 활성 검증

Hee Ju Lee1
,
Ji Hye Moon2*
,
Woo Moon Lee1
,
Sang Gyu Lee1
,
Dong Kum Park2
,
Moo Kyung Yoon1
이 희주1
,
문 지혜2*
,
이 우문1
,
이 상규1
,
박 동금2
,
윤 무경1
1Vegetable Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration
2Urban Agriculture Research Team, National Institute of Horticultural & Herbal Science, Rural Development Administration
1농촌진흥청 국립원예특작과학원 채소과
2농촌진흥청 국립원예특작과학원 도시농업연구팀
*교신저자. *Corresponding Author. 
31 October 2014. pp. 702-709
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Abstract

Luffa cylindrica Roem (sponge gourd) belongs to the Cucurbitaceae family and has been cultivated as an ornamental plant in Korea. Recently, its cultivation area has been increased with an increase in demand for it as an ingredient in cosmetics, herbal medicines, and health supplements. We analyzed inorganic components of sap collected from land race sponge gourds. We also measured antioxidant enzyme activities and antimicrobial activities of the plant, seed, and sap to examine functional properties of sponge gourd. The sap of the sponge gourd contained high levels of K, Ca, P, and Mg, with the most abundant mineral in the sap being K (470 mg･L-1). The amounts of Ca and Mg were 2 and 1.7 times more than those found in cucumber (Cucumis sativus), respectively. Ascorbate peroxidase was more active than catalase and superoxide dismutase in various plant parts of sponge gourd. Antioxidant activities were much lower in stems than in other plant parts such as leaves, roots, flowers, fruits, seeds, and sap. In addition, sap showed a very low level of antimicrobial activity against two food-borne pathogens, Vibrio parahaemdyticus and Propionibacterium acne, and none against the other eight tested food-borne pathogens. Antimicrobial activities against Candida albicans and Malassezia furfur, which causes dermatitis, appeared to be higher in sap than in other parts of sponge gourd plants. Overall, the antimicrobial activity against Malassezia furfur appeared to be higher than against Candida albicans.

Keywords
ascorbate peroxidase
Candida albicans
catalase
Malassezia furfur
superoxide dismutase (SOD)

수세미오이(Luffa cynlindrica)는 박과 채소작물로 우리나라에서는 주로 관상용으로 재배되어 왔다. 그러나 최근에는 미용소재, 한방약재, 건강보조용 등으로 수요가 증가하면서 재배면적이 증가하고 있다. 따라서 본 실험은 수세미오이의 기능성을 구명하기 위하여 재래종 수세미오이를 사용하여 수액의 무기성분을 분석하고 수액과 식물체 부위별 항산화효소 활성과 항균활성을 측정하였다. 수세미오이 수액은 K, Ca, P, Mg이 주요 성분이었으며, K의 함량이 470mg･L-1으로 가장 높았다. Ca과 Mg의 함량은 오이의 약 2배와 1.7배로 높게 나타났다. 수세미오이의 조직별 항산화효소 활성을 분석한 결과 전체적으로 APX 활성이 CAT와 SOD 활성보다 높았다. 식물체 부위별로 비교해 볼 때 항산화효소 활성은 잎, 뿌리, 꽃, 과실, 종자, 수액보다 줄기에서 가장 낮게 나타났다. 수세미오이 수액의 식품유해균 10종에 대한 항균활성은 Vibrio parahaemdyticus와 Propionibacterium acne에 대하여 미미한 활성을 보였을 뿐 그 외 8종의 균에 대해서는 효과가 없었다. 피부염 등 인체 감염을 일으키는 Candida albicans와 Malassezia furfur에 대한 수세미오이의 부위별 항균활성 분석 결과, 수액의 항균활성이 식물체의 다른 부위보다 높게 나타났다. 전체적으로 Malassezia furfur에 대한 항균활성이 Candida albicans에 대한 항균활성보다 높은 것으로 나타났다.

키워드
ascorbate peroxidase
Candida albicans
카탈라아제
Malassezia furfur
superoxide dismutase (SOD)
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 32
  • No :5
  • Pages :702-709
  • Received Date : 2013-05-06
  • Revised Date : 2014-03-10
  • Accepted Date : 2014-04-02
  • DOI :https://doi.org/10.7235/hort.2014.13069
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