Isolation and Characterization of 8-Demethoxy Steffimycins and Generation of 2, 8-Demethoxy Steffimycins in Streptomyces steffisburgemis by the Nogalamycin Biosynthesis Genes

TERO KUNNARI; JAANA TUIKKANEN; ANNE HAUTALA; JUHA HAKALA; KRISTIINA YLIHONKO; PEKKA MÄNTSÄLÄ

doi:10.7164/antibiotics.50.496

Isolation and Characterization of 8-Demethoxy Steffimycins and Generation of 2, 8-Demethoxy Steffimycins in Streptomyces steffisburgemis by the Nogalamycin Biosynthesis Genes

TERO KUNNARI, JAANA TUIKKANEN, ANNE HAUTALA, JUHA HAKALA, KRISTIINA YLIHONKO, PEKKA MÄNTSÄLÄ

Author information

JOURNAL FREE ACCESS

1997 Volume 50 Issue 6 Pages 496-501

DOI https://doi.org/10.7164/antibiotics.50.496

Details

Abstract

Streptomyces steffisburgensis (NRRL 3193, ATCC 27466) is described as a steffimycin producer. Steffimycin belongs to the anthracycline group of aromatic polyketide antibiotics. The structural analysis of the products accumulated by the wild type ATCC 27466 strain revealed three different forms of 8-demethoxy steffimycin suggesting the loss of C-8 hydroxylation/methylation activity. In our approach to generate new anthracycline molecules, we used this strain as a host in gene cloning. The genes encoding the polyketide ketoreductase and aromatase enzymes of nogalamycin biosynthesis caused the production of 2-demethoxy Steffimycins in S. steffisburgensis.

Corresponding author

Register with J-STAGE for free!