DIOLMYCINS, NEW ANTICOCCIDIAL AGENTS PRODUCED BY Streptomyces sp. I. PRI. PRODUCTION, ISOLATION AND PHYSICO-CHEMICAL AND BIOLOGICAL PROPERTIES

NORIKO TABATA; HIROSHI TOMODA; YOKO TAKAHASHI; KATSUJI HANEDA; YUZURU IWAI; H. BOYD WOODRUFF; SATOSHI OMURA

doi:10.7164/antibiotics.46.756

DIOLMYCINS, NEW ANTICOCCIDIAL AGENTS PRODUCED BY Streptomyces sp.

I. PRI. PRODUCTION, ISOLATION AND PHYSICO-CHEMICAL AND BIOLOGICAL PROPERTIES

NORIKO TABATA, HIROSHI TOMODA, YOKO TAKAHASHI, KATSUJI HANEDA, YUZURU IWAI, H. BOYD WOODRUFF, SATOSHI OMURA

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JOURNAL FREE ACCESS

1993 Volume 46 Issue 5 Pages 756-761

DOI https://doi.org/10.7164/antibiotics.46.756

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Abstract

Streptomyces sp. WK-2955, a soil isolate, was found to produce a series of new anticoccidial compounds. Four active compounds, designated diolmycins A1, A2, B1 and B2, were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography, gel filtration on Sephadex LH-20, and preparative HPLC. Diolmycins inhibited the growth of Eimeria tenella in an in vitro assay system using BHK-21 cells as a host. No schizont in the cells was observed at concentrations of 0.02-2.0μg/ml for diolmycin Al, at 0.2-2.0μg/ml for diolmycin A2, and at 20μg/ml for diolmycins Bl and B2.

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