Supplementary material from "Tumour-suppressor microRNAs regulate ovarian cancer cell physical properties and invasive behaviour"
Posted on 2016-11-16 - 14:54
The activities of pathways that regulate malignant transformation can be influenced by microRNAs (miRs). Recently, we showed that increased expression of five tumour-suppressor miRs: miR-508-3p, miR-508-5p, miR-509-3p, miR-509-5p and miR-130b-3p, correlate with improved clinical outcomes in human ovarian cancer patients, and that miR-509-3p attenuates invasion of ovarian cancer cell lines. Here, we investigate the mechanism underlying this reduced invasive potential by assessing the impact of these five miRs on the physical properties of cells. Human ovarian cancer cells (HEYA8, OVCAR8) that are transfected with miR mimics representing these five miRs exhibit decreased invasion through collagen matrices, increased cell size and reduced deformability as measured by microfiltration and microfluidic assays. To understand the molecular basis of altered invasion and deformability induced by these miRs, we use predicted and validated mRNA targets that encode structural and signalling proteins that regulate cell mechanical properties. Combined with image analysis of F-actin in single cells, our results suggest that these tumour-suppressor miRs may alter cell physical properties by regulating the actin cytoskeleton. Our findings provide biophysical insights into how tumour-suppressor miRs can regulate the invasive behaviour of ovarian cancer cells, and identify potential therapeutic targets that may be implicated in ovarian cancer progression.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Chan, Clara K.; Pan, Yinghong; Nyberg, Kendra; A. Marra, Marco; Lim, Emilia L.; J. M. Jones, Steven; et al. (2016). Supplementary material from "Tumour-suppressor microRNAs regulate ovarian cancer cell physical properties and invasive behaviour". The Royal Society. Collection. https://doi.org/10.6084/m9.figshare.c.3577190.v1
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (11)
CC
Clara K. Chan
YP
Yinghong Pan
KN
Kendra Nyberg
MA
Marco A. Marra
EL
Emilia L. Lim
SJ
Steven J. M. Jones
DM
Dianna Maar
EA
Ewan A. Gibb
PH
Preethi H. Gunaratne
AG
A. Gordon Robertson
AR
Amy C. Rowat