3.Immunoblotting.xlsx (42.42 kB)
Genome-wide chromatin and gene expression profiling during memory formation and maintenance in adult mice. [Data Record 3: Immunoblotting]
Version 4 2016-06-28, 10:06
Version 3 2016-06-27, 09:30
Version 2 2016-06-13, 14:47
Version 1 2016-06-10, 16:34
dataset
posted on 2016-06-28, 10:06 authored by Tonatiuh Pena CentenoTonatiuh Pena Centeno, orr shomroniorr shomroni, Ramon VidalRamon Vidal, Magali Hennion, Rashi Halder, Raza-Ur Rahman, Stefan BonnDescription
The file contains the immunoblotting results for chromatin markers H3K9ac, H3K4me3, H3K27ac, H3K27me3 and H4K12ac for tissues ACC and CA1, and one immunoblotting result for CA1 neuronal H3K9ac for conditions Naive (N), context at 1h (C) and context-shock at 1h (CS). Each table contains the tested sample names (based on their condition), chromatin mark, immunoblot channel, number ID and signal strength, total, area and background. Each replicate had its signals compared between the chromatin mark and H4 signal immunoblotting, resulting in the ratio signal and standard deviation between the signals. The average signal for each condition N, C and CS and standard deviation of said signals were also recorded, and finally, some immunoblots run twice for the same chromatin mark had their averages and standard deviations calculated together.
The file contains the immunoblotting results for chromatin markers H3K9ac, H3K4me3, H3K27ac, H3K27me3 and H4K12ac for tissues ACC and CA1, and one immunoblotting result for CA1 neuronal H3K9ac for conditions Naive (N), context at 1h (C) and context-shock at 1h (CS). Each table contains the tested sample names (based on their condition), chromatin mark, immunoblot channel, number ID and signal strength, total, area and background. Each replicate had its signals compared between the chromatin mark and H4 signal immunoblotting, resulting in the ratio signal and standard deviation between the signals. The average signal for each condition N, C and CS and standard deviation of said signals were also recorded, and finally, some immunoblots run twice for the same chromatin mark had their averages and standard deviations calculated together.
