Doyle, Andrew D.; Wang, Francis W.; Matsumoto, Kazue; Yamada, Kenneth M. (2021). CIL:13161, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0M0445F

Transition from 1D to 2D migration: regulation of cellular phenotype by ECM topography. Formation of multiaxial lamellae and cell spreading during the transition from 1D to 2D migration. 2D matrices were constructed by uniform coating with extracellular matrix (ECM). 1D surfaces were made by using a 2 photon confocal microscope to pattern a polyvinyl alchohol (PVA) coated coverslip. Following gluteraldehyde quenching, ECM proteins were absorbed onto the PVA surface. Movie is video 6 from JCB 2009, 184:481-490

• 2021

• Doyle, Andrew D.
• Matsumoto, Kazue
• Wang, Francis W.
• Yamada, Kenneth M.

Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 2 min and shown at 15 frames per second.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: custom-made smoothing, sharpening, and convolution filters using MetaMorph software
Data qualification: Processed

• Cell Image Library Group ID: 9565

• Mus musculus

• Cell
• Fibroblast

• Cell migration
• NIH/3T3
• Substrate-dependent cell migration, cell attachment to substrate

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 13161

Doi: https://doi.org/10.6075/J0M0445F

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13161

Reference

• JCB 2009, 184:481-490 https://www.ncbi.nlm.nih.gov/pubmed/?term=19221195

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2022-08-12