Wittmann, Torsten; Bokoch, Gary M.; Waterman-Storer, Clare M. (2021). CIL:11841, Potorous tridactylus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09P30C6

Microtubules in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 4, B and D of associated paper (J Cell Biol, 161:845-851, 2003).

• 2021

• Bokoch, Gary M.
• Waterman-Storer, Clare M.
• Wittmann, Torsten

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: X-Rhodamine
Processing history: unprocessed raw data
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 4105

• Potorous tridactylus

• Epithelial cell
• Microtubule

• Activated Rac
• Microtubule polymerization
• PAK inhibition
• Pt K1 (NBL-3)

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 11841

Doi: https://doi.org/10.6075/J09P30C6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11841

Reference

• J Cell Biol, 161:845-851, 2003. https://www.ncbi.nlm.nih.gov/pubmed/?term=12796474
• Wittmann T, Bokoch GM, Waterman-Storer CM. Regulation of leading edge microtubule and actin dynamics downstream of Rac1. J Cell Biol. 2003 Jun 9;161(5):845-51. https://doi.org/10.1083/jcb.200303082

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2022-08-12