BACK TO CONTENTS   |    PDF   |    PREVIOUS   |    NEXT

Title

 

 

 

 

Comparative characterization of commercially important xylanase enzymes

 

Authors

Neelima Arora, Amit Kumar Banerjee, Srilaxmi Mutyala, U. S. N Murty*

 

Affiliation

Bioinformatics Group, Biology Division, Indian Institute of Chemical Technology, Hyderabad-500007, A.P., India

 

Email

 

murty_usn@yahoo.com

 

Article Type

Hypothesis

 

Date

 

Received April 30, 2009; accepted June 07, 2009; published August 05, 2009

 

Abstract

 

 

Xylanase is an industrially important enzyme having wide range of applications especially in paper industry. It is crucial to gain an understanding about the structure and functional aspects of various xylanases produced from diverse sources. In this study, a bioinformatics and molecular modeling approach was adopted to explore properties and structure of Xylanases. Physico-chemical properties were predicted and prediction of motifs, disulfide bridges and secondary structure was performed for functional characterization. Apart from these analyses, three dimensional structures were constructed and stereo-chemical quality was evaluated by different structure validation tools. Comparative catalytic site analysis and assessment was performed to extract information about the important residues. Asn72 was found to be the common residue in the active sites of the proteins P35809 and Q12603.

 

Keywords

Xylanase, Bioinformatics, sequence analysis, structure prediction; function

 

Citation

Arora et al., Bioinformation 3(10): 446-453 (2009)

 

Edited by

P. Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

License

 

 

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.