Distribution of high-risk types of human papillomavirus compared to histopathological findings in cervical biopsies in women

Vitković, Leonida; Perišić, Ž.; Trajković, G.; Mijović, M.; Savić, S.; Leštarević, S.; Đerković, B.

doi:10.5937/pramed1504039V

Akcije

dodaj u Moj izbor [0]

kako citirati ovaj članak
prikaži na oba jezika
podeli ovaj članak

Direktan link

Metrika

citati u SCIndeksu: [1]
citati u CrossRef-u:[1]
citati u Google Scholaru:[]
posete u poslednjih 30 dana:56
preuzimanja u poslednjih 30 dana:54

Sadržaj

članak: 1 od 1

Praxis medica

2015, vol. 44, br. 4, str. 39-44

Distribucija visokorizičnih tipova humanih papiloma virusa u odnosu na patohistološke nalaze cervikalnih biopsija kod žena

Vitković Leonida^a

, Perišić Ž.^b, Trajković G.^c, Mijović M.^d

, Savić S.^a

, Leštarević S.^a

, Đerković B.^d

^aUniverzitet u Prištini sa privremenim sedištem u Kosovskoj Mitrovici, Medicinski fakultet, Institut za histologiju sa embriologijom, Srbija
^bGinekološko-akušerska klinika 'Narodni front', Beograd, Srbija
^cUniverzitet u Beogradu, Medicinski fakultet, Institut za medicinsku statistiku i informatiku, Srbija
^dUniverzitet u Prištini sa privremenim sedištem u Kosovskoj Mitrovici, Medicinski fakultet, Institut za patologiju, Srbija

e-adresa: leonidavitkovic@yahoo.com

Ključne reči: cervix uteri; HPV; LSIL; HSIL; Ca planocellulare

Sažetak

Uvod: U preko 99% slučajeva pojavi karcinoma grlića materice predhodi perzistentna infekcija cervikalnog epitela visoko-rizičnim onkogenim tipovima humanih papiloma virusa (HPV). Cilj rada je bio ispitati distribuciju visokorizičnih onkogenih tipova HPVu odnosu na patohistološke nalaze cervikalnih biopsija kod žena. Materijal i metode: Ispitano je 56 žena sa sumnjom na postojanje premalignih i malignih cervikalnih lezija, zbog suspektnog kolposkopskog i citološkog nalaza (Papanicolau testa). Kod svih ispitanica je iz cervikalnog brisa urađena HPV tipizacija metodom 'in situ' hibridizacije na tipove visokog rizika HPV 16, 18, 31 i 33. Sa cerviksa je uzeta biopsija i histološki nalaz cervikalne biopsije predstavljao je 'zlatni standard' u analizi materijala. Rezultati: Od 56 ispitanica kod 34 (60,7%) histološki su detektovane premaligne ili maligne lezije na grliću materice: 17 ispitanica imalo je LSIL, 13 HSIL, a kod 4 je dijagnostikovan planocelularni karcinom. Pozitivan HPV test imalo je 47 (84%) ispitanica i kod njih je dokazano prisustvo jednog ili više tipova HPV. Najučestaliji tip virusa bio je HPV 16 koji je detektovan kod 27 (48,2%) ispitanica, zatim HPV 31 kod 26 (46,4%), pa HPV 18 kod 18_ (32,1%) i HPV 33 kod 4 (7,1%) ispitanice. Infekcija onkogenim tipom HPV16 bila je značajno češća kod ispitanica sa HSIL-om i karcinomom (p<0,001), dok je infekcija tipom HPV 31 bila značajno češća kod ispitanica sa LSIL-om i cervicitisom (p=0,003). Distribucija HPV 18 i HPV 33 statistički se značajno nije razlikovala kod ispitanica sa različitim histološkim nalazima (HPV 18 p=0,41; HPV 33 p=1,0). Zaključak: Na osnovu naših rezultata možemo zaključiti da postoji dobra korelacija HPV infekcije, premalignih cervikalnih lezija i karcinoma grlića materice. Učestalost HPV infekcije tipom 16 raste sa težinom cervikalne lezije i to je najčešće detektovani visokorizični onkogeni tip virusa kod žena sa težim cervikalnim lezijama tipa HSIL i karcinomom. HPV 31 je najčešće detektovan visokorizični tip HPV-a kod žena sa blažim premalignim promena tipa LSIL i kod cervicitisa. Smatramo da žene inficirane visokorizičnim onkogenim tipovima HPV, iako nemaju histološki dijagnostikovanu cervikalnu leziju, treba češće kolposkopski i citološki (Papanicolau test) kontrolisati, zbog moguće progresije bolesti u teži stepen.

Abstract

Introduction: In over of 99% cases of cervical cancer its appearing is preceded by persistent cervical epithelium infection caused by high-risk oncogenic types of human papillomavirus (HPV). The aim of the study was to examine the distribution of high-risk oncogenic HPV types compared to patohistological diagnoses of cervical diseases in women. Materials and methods: The study included 56 women with suspected premalignant and malignant cervical lesions, due to suspected colposcopic and cytological findings (Papanicolaou test). The HPV typing by 'in situ' hybridization method on high-risk HPV types 16, 18, 31 and 33 was performed in all patients from cervical smear as well as cervical biopsy. Histological findings of cervical biopsy was a 'gold standard' in the analysis of materials. Results: Histologically detected premalignant or malignant changes of the cervix were found at 34 (60.7%) of all 56 examined women: 17 of them had LSIL, 13 of them had HSIL, while 4 had squamous cell carcinoma. A positive HPV test had a 47 (84%) of them with a prove of the presence of one or more types of HPV. The most common type of virus was HPV 16 and it was detected in 27 (48.2%) women, followed by HPV 31 that was detected in 26 (46.4%) women, HPV 18 in 18 (32.1%) of women and HPV 33 in 4 (7.1%) women. The infection caused by oncogenic type HPV16 was significantly more frequent in patients with HSIL and cervical cancer (p<0,001), while the infection caused by oncogenic type HPV 31 was significantly more frequent in patients with LSIL and cervicitis (p=0,003). The distribution of HPV 18 and HPV 33 types was not statistically significantly different in patients with different histological findings (HPV 18, p = 0.41; HPV 33, p = 1.0). Conclusion: Based on our results we can conclude that there is a good correlation of HPV infection with pre-malignant cervical lesions and cervical cancer. The incidence of HPV type 16 infection increased with severity of cervical lesions and it is usually detected high-risk oncogenic type virus in women with severe cervical lesions type like HSIL and cancer are. HPV 31 is the most common high-risk type of HPV of mild type lesions, like LSIL and cervicitis are. We believe that women infected by high-risk oncogenic HPV types, although without histologically diagnose of cervical lesion, should be more frequent control by colposcopy and cytology (Papanicolaou) test, because of possible disease progression to a more advanced level.

	Reference
1	Benton, E.C., Arends, M.J. (1996) Human papillomavirus in the immunosuppressed. u: Lacey C. [ur.] Papillomavirus reviews Current research on papillomaviruses, Leeds University Press, pp. 271-279
	Clavel, C., Masure, M., Bory, J., Putaud, I., Mangeonjean, C., Lorenzato, M., Nazeyrollas, P., Gabriel, R., Quereux, C., Birembaut, P. (2001) Human papillomavirus testing in primary screening for the detection of high-grade cervical lesions: a study of 7932 women. British Journal of Cancer, 84(12): 1616-1623
2	Clifford, G.M., Smith, J.S., Plummer, M., Munoz, N., Franceschi, S. (2003) Human papillomavirus types in invasive cervical cancer worldwide: A meta-analysis. Br J Cancer, 88(1): 63-73
	Day, P.M., Thompson, C.D., Buck, C.B., Pang, Y.S., Lowy, D.R., Schiller, J.T. (2007) Neutralization of human papillomavirus with monoclonal antibodies reveals different mechanisms of inhibition. Journal of virology, 81(16): 8784-92
	de Villiers, E.-M., Schneider, A., Gross, G., Hausen, H. zur (1986) Analysis of benign and malignant urogenital tumors for human papillomavirus infection by labelling cellular DNA. Medical Microbiology and Immunology, 174(6): 281-286
2	de Villiers, E., Fauquet, C., Broker, T.R., Bernard, H., zur Hausen, H. (2004) Classification of papillomaviruses. Virology, 324(1): 17-27
1	Doorbar, J. (2006) Molecular biology of human papillomavirus infection and cervical cancer. Clinical science, 110(5): 525-41
	Evander, M., Frazer, I.H., Payne, E., Qi, Y.M., Hengst, K., McMillan, N.A. (1997) Identification of the alpha6 integrin as a candidate receptor for papillomaviruses. Journal of virology, 71(3): 2449-56
	Franco, Eduardo L., Villa, Luisa L., Sobrinho, João P., Prado, José M., Rousseau, M., Désy, M., Rohan, Thomas E. (1999) Epidemiology of Acquisition and Clearance of Cervical Human Papillomavirus Infection in Women from a High‐Risk Area for Cervical Cancer. Journal of Infectious Diseases, 180(5): 1415-1423
1	Gharizadeh, B., Kalantari, M., Garcia, C.A., Johansson, B., Nyrén, P. (2001) Typing of human papillomavirus by pyrosequencing. Laboratory investigation; a journal of technical methods and pathology, 81(5): 673-9
	Halfon, P., Trepo, E., Antoniotti, G., Bernot, C., Cart-Lamy, P., Khiri, H., Thibaud, D., Marron, J., Martineau, A., Penaranda, G. (2006) Prospective Evaluation of the Hybrid Capture 2 and AMPLICOR Human Papillomavirus (HPV) Tests for Detection of 13 High-Risk HPV Genotypes in Atypical Squamous Cells of Uncertain Significance. Journal of Clinical Microbiology, 45(2): 313-316
	Hausen, H.zur (2002) Papillomaviruses and cancer: from basic studies to clinical application. Nature Reviews Cancer, 2(5): 342-350
1	Heley, S. (2003) Human papillomavirus: Beware the infection you can't see. Ust Fam Physician, 32, 311-5
	Hildesheim, A., Schiffman, M.H., Gravitt, P.E., Glass, A.G., Greer, C.E., Zhang, T., Scott, D.R., Rush, B.B., Lawler, P., Sherman, M.E. (1994) Persistence of type-specific human papillomavirus infection among cytologically normal women. Journal of Infectious Diseases, 169(2): 235-40
	Ho, G.Y., Palan, P.R., Basu, J., Romney, S.L., Kadish, A.S., Mikhail, M., Wassertheil-Smoller, S., Runowicz, C., Burk, R.D. (1998) Viral characteristics of human papillomavirus infection and antioxidant levels as risk factors for cervical dysplasia. International journal of cancer, 78(5): 594-9
	IARC (1995) IARC monographs on the evaluation of carcinogenic risks to humans, vol. 64: Human papillomaviruses. Lyon: International Agency for Research on Cancer
	IARC (2005) IARC handbooks of cancer research prevention, vol. 10: Cervix cancer screening. Lyon
	IARC (2007) IARC monographs on the evaluation of carcinogenic risks to humans, vol. 90: Human Papillomaviruses. Lyon
1	Kalof, A.N., Cooper, K. (2006) p16INK4a Immunoexpression: Surrogate Marker of High-risk HPV and High-grade Cervical Intraepithelial Neoplasia. Advances in Anatomic Pathology, 13(4): 190-194
	Koutsky, L., Brown, W.V. (1997) Epidemiology of genital human papillomavirus infection. American journal of medicine, 102(5A): 3-8
1	Krstić, Lj. (2005) Papillomaviridae. u: Specijalna virusologija, Beograd: Savremena administracija, 129-31
1	Lacey, C.J. (1992) Assessment of exposure to sexually transmitted agents other than human papillomavirus. u: Munoz N., Bosch F.X., Shah K.V., Meheus A. [ur.] The epidemioloy of cervical cancer and human papillomavirus (IARC Scientific Publications No. 119), Lyon, pp. 93-105
1	Lalević-Vasić, B.M., Medenica, Lj.M., Nikolić, M.M. (2006) Virusne infekcije. u: Dermatovenerologija, Beograd: Savremena administracija
	Letian, T., Tianyu, Z. (2010) Cellular receptor binding and entry of human papillomavirus. Virology Journal, 7(1): 2
	Lorincz, A.T., Reid, R., Jensen, A.B., Greenberg, M.D., Lancaster, W., Kurman, R.J. (1992) Human papillomavirus infection of the cervix: Relative risk associations of 15 common anogenital types. Ostret Gynecol, 79, 328
1	Lowy, D.R., Schiller, J.T. (2006) Prophylactic human papillomavirus vaccines. Journal of clinical investigation, 116(5): 1167-73
	Milutin-Gasperov, N., Sabol, I., Halec, G., Matovina, M., Grce, M., Pavić, L.L., Klarić, P., Lugović, L. (2007) Retrospective study of the prevalence of high-risk human papillomaviruses among Croatian women. Collegium antropologicum, 31 Suppl 2(1): 89-96
	Moscicki, A. (2005) Impact of HPV infection in adolescent populations. Journal of Adolescent Health, 37(6): S3-S9
	Mulvany, N.J., Allen, D.G., Wilson, S.M. (2008) Diagnostic utility of p16INK4a: a reappraisal of its use in cervical biopsies. Pathology, 40(4): 335-344
	Munoz, N., Bosch, F.X., de Sanjose, S., Tafur, L., Izarzugaza, I., Gili, M., Viladiu, P., Navarro, C., Martos, C., Ascunce, N. (1992) The causal link between human papillomavirus and invasive cervical cancer: A population-based case-control study in colombia and spain. International Journal of Cancer, 52(5): 743-749
1	Muñoz, N., Bosch, F. X., de Sanjosé, S., Herrero, R., Castellsagué, X., Shah, K.V., Snijders, P.J.F., Meijer, C.J.L.M. (2003) Epidemiologic Classification of Human Papillomavirus Types Associated with Cervical Cancer. New England Journal of Medicine, 348(6): 518-527
	Murphy, N., Ring, M., Killaea, A.G., Uhlmann, V., o'donovan M., Mulcahy, F., i dr. (2003) p16INK4A as a marker for cervical dyskariosis: CIN and cGIN in cervical biopsies and Thin PrepTM smears. J Clin Pathol, 56: 53-63
	Östör, A.G. (1993) Natural History of Cervical Intraepithelial Neoplasia. International Journal of Gynecological Pathology, 12(2): 186
	Parkin, D. M., Bray, F. (2006) Chapter 2: The burden of HPV-related cancers. Vaccine, 24: S11-S25
2	Pater, M.M., Pater, A. (1985) Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology, 145(2): 313-8
	Popović-Lazić, J. (2006) Histološka slika cervikalnih intraepitelnih neoplazija. u: Stanimirović B. [ur.] Dijagnostika i lečenje oboljenja cerviksa, vagine i vulve - kolposkopski atlas, Beograd: Elit-Medica, 23-26
1	Queiroz, C., Silva, T.C., Alves, V.A.F., Villa, L.L., Costa, M.C., Travassos, A.G., Filho, J.B.A., Studart, E., Cheto, T., de Freitas, L.A.R. (2006) P16(INK4a) expression as a potential prognostic marker in cervical pre-neoplastic and neoplastic lesions. Pathology, research and practice, 202(2): 77-83
	Reddout, N., Christensen, T., Bunnell, A., Jensen, D., Johnson, D., o'Malley Susan,, Kingsley, K. (2007) High risk HPV types 18 and 16 are potent modulators of oral squamous cell carcinoma phenotypes in vitro. Infectious Agents and Cancer, 2(1): 21
2	Schiffman, M.H., Bauer, H.M., Hoover, R.N., Glass, A.G., Cadell, D.M., Rush, B.B., Scott, D.R., Sherman, M.E., Kurman, R.J., Wacholder, S. (1993) Epidemiologic evidence showing that human papillomavirus infection causes most cervical intraepithelial neoplasia. J Natl Cancer Inst, 85(12): 958-64
	Spoden, G., Kuhling, L., Cordes, N., Frenzel, B., Sapp, M., Boller, K., Florin, L., Schelhaas, M. (2013) Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry. Journal of Virology, 87(13): 7765-7773
	Stanimirović, B., Atanacković, J., Babić, M., Berisavac, M., Bošković, V., Vujkov, T., i dr. (2006) Dijagnostika i lečenje oboljenja cerviksa, vagine i vulve - kolposkopski atlas. Beograd: Elit-Medica
	Stojanović, J., Magić, Z., Milačić, M., Nenadić, D., Stanimirović, B., Vukičević, D. (2002) Distribution of high-risk HPV tyres in Yugoslav women with cervical neoplasia. J Buon., 7: 251-256
	Subramanya, D., Grivas, P.D., Flank, S. (2008) HPV and cervical cancer: updates on an established relationship. Postgraduate medicine, 120(4): 7-13
5	Šimunić, V., i dr. (2001) Ginekologija. Zagreb: Naklada Ljevak
	Tindle, R. (1996) Human papillomarivus vaccines for cervical cancer. Current Opinion in Immunology, 8(5): 643-650
	Uuskűla, A., Kals, M., Kosenkanius, L., Mcnutt, L.A., de Hovitz, J. (2010) Population based type-specific, prevalence of high-risk human papillomavirus infection in Estonia. Infectius Diseases, 10, 63
	Vinokurova, S., Wentzensen, N., Kraus, I., Klaes, R., Driesch, C., Melsheimer, P., Kisseljov, F., Durst, M., Schneider, A., Doeberitz, M. von K. (2008) Type-Dependent Integration Frequency of Human Papillomavirus Genomes in Cervical Lesions. Cancer Research, 68(1): 307-313
2	Vitković, L., Mijović, M., Vukićević, D., Đerković, B., Mitić, N., Ilić, A., Trajković, G., Jakovljević, S. (2015) Histološko-citološka korelacija nalaza i pouzdanost papanicolau testa u otkrivanju premalignih i malignih promena na grliću materice. Praxis medica, vol. 44, br. 1, str. 23-31
1	Vitković, L. (2011) Značaj molekularnih markera u histološkoj i citološkoj analizi epitela grlića materice. Priština - Kosovska Mitrovica, Doktorska disertacija
1	Vitković, L., Mitić, B.N., Piperski, V., Trajković, G., Anđeliković, Z., Vukićević, D., Stević, S. (2011) Ekspresija proteina p16INK4a u normalnom i displastično promenjenom epitelu grlića materice. Praxis medica, 39(3-4); 13-17
	Vitković, L., Perišić, Ž., Mitić, N., Piperski, V., Trajković, G. (2011) Importance of P16INK4a in cervical cytology. u: 36th European Congress of Cytology, Cytopathology 2011, Istambul, P-017, 60
	Vitković, L., Piperski, V., Mitić, N., Anđelković, Z., Savić, S., Leštarević, S., Rančić, G. Značaj ekspresije proteina p16ink4a u histološkoj analizi epitela grlića materice. u: III Kongres Srpskog anatomskog društva Srbije sa međunarodnim učešćem, 6-8 septembar 2012, Zbornik sažetaka, Niš: Medicinski fakultet, 171-172
2	von Knebel, D.M. (2002) New markers for cervical dysplasia to visualise the genomic chaos created by aberrant oncogenic papillomavirus infections. European journal of cancer, 38(17): 2229-42
1	zur Hausen, H. (2000) Papillomaviruses causing cancer: evasion from host-cell control in early events in carcinogenesis. Journal of the National Cancer Institute, 92(9): 690-8
1	Žuža-Jurica, S., Dabo, J., Utješinović-Gudelj, V., Oluić-Kabalin, G., Mozetič, V. (2009) Humani papiloma virus: prevencija, cijepljenje, liječenje. Medicina, 45(1), 49-55

O članku

jezik rada: srpski
vrsta rada: stručni članak
DOI: 10.5937/pramed1504039V
objavljen u SCIndeksu: 27.04.2016.

Povezani članci

Nema povezanih članaka