In vitro study of human multiple myeloma cell transfected with soluble vascular endothelial growth factor receptor-1 gene
Dong Zheng, Juan Li, Jun-Ru Liu, Zhen-Hai Zhou , Jing-Li Gu
Department of Hematology, The First Affiliated Hospital of Sun Yat-sen University,Guangzhou, 510080, Guangdong, P. R. China. Email: luliyuan@tom.com
[Abstract] Background and Objective: Vascular endothelial growth factor (VEGF) is the most important angiogenic factor of multiple myeloma (MM). This study was to investigate the effect of transfection of human soluble vascular endothelial growth factor receptor-1 (sFlt-1) gene on the growth of human MM cell line RPMI8226. Methods: The sFlt-1 gene was obtained beforehand and preserved as recombinant plasmid pcDNA3-sFlt-1. The recombinant plasmid pcDNA3-sFlt-1 was transfected into RPMI8226 cells by lipofectamine 2000. The expression of sFlt-1 gene was identified by RT-PCR and ELISA. The effect of sFlt-1 protein on cell growth and VEGF expression in RPMI8226 cells was investigated by MTT and ELISA, respectively. Results: The recombinant plasmid pcDNA3-sFlt-1 was successfully transfected into RPMI8226 cells. And the sFlt-1 protein expression was identified by ELISA. The growth of RPMI8226 cells was inhibited and the VEGF level in the culture supernatant reduced. Conclusion: RPMI8226 cells can express sFlt-1 protein with high biological activity when transfected by the sFlt-1 gene, which inhibits the growth of RPMI8226 cells.
Chinese Journal of Cancer 2010, Volume: 29, Issue 1, Page: 61-
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