A 4-methylsterol was isolated from the unsaponifiables of rice-bran oil by preparative thin-layer chromatography (TLC), and identified as cycloeucalenol [4α, 14α-dimethyl-9, 19-cyclopropane-24 (28) -methylene cholestan-3β-ol] by TLC, IR, NMR and combined GC-MS, the occurrence of which in rice-bran oil had not yet been reported.
The cycloeucalenol and its acetate showed mp 141142°C and 110111°C, respectively. Cycloeucalanol derived from the cycloeucalenol by hydrogenation and its acetate showed mp 147-148°C and 112113°C, respectively.