Fruiting bodies of Hericium erinaceus (Bull.) Pers. – a new source of water-insoluble (1→3)-α-d-glucan

Adrian Wiater, Adam Choma, Iwona Komaniecka, Małgorzata Pleszczyńska, Marek Siwulski, Paulina Polak, Grzegorz Janusz, Janusz Szczodrak

Abstract


A water-insoluble polysaccharide (WIP) was isolated from the fruiting bodies of Hericium erinaceus HE01 by an alkaline solution with the yield of 5%. Structural and compositional analyses by total acid hydrolysis, methylation analysis, FT-IR, FT-Raman, and 1H NMR spectroscopy as well as other instrumental techniques showed predominantly glucose linked by α-glycosidic bonds and small amounts of mannose, xylose, rhamnose, galactose, and ribose. The methylation analysis showed that (1→3)-linked Glcp is the major constituent (70.8%) of the polymer, while the 3,4 substituted d-Glcp represents the main branching residue of the glucan. The presence of (1→3)-α-d-glucan in the hyphae of H. erinaceus was additionally confirmed by the use of specific fluorophore-labeled antibodies.

Keywords


Hericium erinaceus; (1→3)-α-d-glucan; cell wall; fruiting body; carbohydrate analysis

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References


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DOI: https://doi.org/10.5586/asbp.3506

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