SPARC is a Major Secretory Gene Expressed and Involved in the Development of Proliferative Diabetic Retinopathy

Kazuhisa Watanabe; Fumiki Okamoto; Tomotaka Yokoo; Kaoruko Tada Iida; Hiroaki Suzuki; Hitoshi Shimano; Tetsuro Oshika; Nobuhiro Yamada; Hideo Toyoshima

doi:10.5551/jat.E711

Original Articles

SPARC is a Major Secretory Gene Expressed and Involved in the Development of Proliferative Diabetic Retinopathy

Kazuhisa Watanabe, Fumiki Okamoto, Tomotaka Yokoo, Kaoruko Tada Iida, Hiroaki Suzuki, Hitoshi Shimano, Tetsuro Oshika, Nobuhiro Yamada, Hideo Toyoshima

Author information

Kazuhisa Watanabe
Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba.
Fumiki Okamoto
Department of Ophthalmology, Institute of Clinical Medicine, University of Tsukuba.
Tomotaka Yokoo
Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba.
Division of Endocrinology and Metabolism, Department of Medicine, Saitama Medical Center, Jichi Medical University.
Kaoruko Tada Iida
Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba.
Hiroaki Suzuki
Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba.
Hitoshi Shimano
Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba.
Tetsuro Oshika
Department of Ophthalmology, Institute of Clinical Medicine, University of Tsukuba.
Nobuhiro Yamada
Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba.
Hideo Toyoshima
Department of Internal Medicine (Endocrinology and Metabolism), Graduate School of Comprehensive Human Sciences, University of Tsukuba.
Division of Endocrinology and Metabolism, Department of Medicine, Saitama Medical Center, Jichi Medical University.

Keywords: Proliferative diabetic retinopathy, Neovascularization, Secreted protein acidic and rich in cystein

JOURNAL OPEN ACCESS

2009 Volume 16 Issue 2 Pages 69-76

DOI https://doi.org/10.5551/jat.E711

View "Advance Publication" version (April 30, 2009).

Details

Abstract

Aim: Neovascularization is an important event in proliferative diabetic retinopathy (PDR), where various secretory proteins including multiple growth factors are considered to be involved in this process. We searched for secretory proteins expressed in a surgical specimen obtained from the eyes of patients with PDR.
Methods: We developed the oligo-cap signal sequence trap (SST) strategy which enables us to screen for secretory or membrane proteins from a minimal starting material. Using this method, we were able to screen a cDNA library constructed from a surgical specimen obtained from the eyes of the patients with PDR.
Results: Majority of the cloned cDNAs turned out to encode secreted protein acidic and rich in cystein (SPARC), strongly suggesting that SPARC is highly expressed in PDR. Analysis of vitreous fluid from various patients has shown that the concentration of SPARC protein is increased in patients with PDR. Furthermore, subretinal injection of recombinant SPARC adenovirus induced PDR-like changes in the rat eye.
Conclusions: Our results strongly suggested that SPARC is involved in the development of diabetic retinopathy (DR).

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