Supporting data for "Single molecule, full-length transcript sequencing provides insight into the extreme metabolism of ruby-throated hummingbird Archilochus colubris"
Dataset type: Transcriptomic
Data released on February 07, 2018
Workman RE; Myrka AM; Tseng E; Wong GW; Welch KC; Timp W (2018): Supporting data for "Single molecule, full-length transcript sequencing provides insight into the extreme metabolism of ruby-throated hummingbird Archilochus colubris" GigaScience Database. https://doi.org/10.5524/100403
Hummingbirds oxidize ingested nectar sugars directly to fuel foraging but cannot sustain this fuel use during fasting periods, such as during the night or during long-distance migratory flights. Instead, fasting hummingbirds switch to oxidizing stored lipids, derived from ingested sugars. The hummingbird liver plays a key role in moderating energy homeostasis and this remarkable capacity for fuel switching. Additionally, liver is the principle location of de novo lipogenesis, which can occur at exceptionally high rates, such as during premigratory fattening. Yet understanding how this tissue and whole organism moderates energy turnover is hampered by a lack of information regarding how relevant enzymes differ in sequence, expression, and regulation.
We generated a de novo transcriptome of the hummingbird liver using PacBio full-length cDNA sequencing (Iso-Seq), yielding a total of 8.6Gb of sequencing data, or 2.6M reads from 4 different size fractions. We analyzed data using the SMRTAnalysis v3.1 Iso-Seq pipeline, then clustered isoforms into gene families to generate de novo gene contigs using Cogent. We performed orthology analysis to identify closely related sequences between our transcriptome and other avian and human gene sets. Finally, we closely examined homology of critical lipid metabolism genes between our transcriptome data and avian and human genomes.
Our results leverage cutting-edge technology and a novel bioinformatics pipeline to provide a first direct look at the transcriptome of this incredible organism.
Additional details
Read the peer-reviewed publication(s):
- Workman, R. E., Myrka, A. M., Wong, G. W., Tseng, E., Welch, K. C., & Timp, W. (2018). Single-molecule, full-length transcript sequencing provides insight into the extreme metabolism of the ruby-throated hummingbird Archilochus colubris. GigaScience, 7(3). https://doi.org/10.1093/gigascience/giy009 (PubMed:29618047)
Additional information:
https://github.com/reworkman/hummingbird
http://dx.doi.org/10.5281/zenodo.1054086
Accessions (data included in GigaDB):
PROJECT: PRJNA369257
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Table SettingsSample ID | Common Name | Scientific Name | Sample Attributes | Taxonomic ID | Genbank Name |
---|---|---|---|---|---|
SAMN06282498 | Archilochus colubris | Description:Liver transcriptome of Ruby-throated H... Analyte type:RNA Sex:male ... |
190676 | ||
A.colubris_Illumia | Archilochus colubris | Environmental medium:animal trap:[ENVO:01000975]; ... Description:Liver transcriptome of Ruby-throated H... Analyte type:RNA ... |
190676 |
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Table SettingsFile Name | Description | Sample ID | Data Type | File Format | Size | Release Date | File Attributes | Download |
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Readme | TEXT | 4.57 kB | 2018-02-07 | MD5 checksum: 1bf14e3f1425de55b6f1096b8f54bf95 |
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Code associated with analysis of Hummingbird transcriptomic data. Contains parameters for BUSCO, GMAP, BLAST, ANGEL, and basic scripts for data manipulation | GitHub archive | archive | 9.93 kB | 2018-02-07 | MD5 checksum: 6e2f85b08a9f303f79d5cc8abcfa647a |
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Alignments of Cogent gene families | Alignments | BAM | 19.01 MB | 2018-02-07 | MD5 checksum: 1aa599285663ea942f086abfd8077a94 |
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Orthomcl coortholog pairs list | Text | TEXT | 37.40 MB | 2018-02-07 | MD5 checksum: 8b20727d4314f7c81653d71bae2af40d |
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Orthomcl paralog pairs list | Text | TEXT | 613.59 MB | 2018-02-07 | MD5 checksum: 5f179b6a7d62fd658b401580e648e106 |
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Orthomcl ortholog pairs list | Text | TEXT | 102.46 MB | 2018-02-07 | ||||
Aves: TBLASTN output file (Cogent) | Text | UNKNOWN | 137.97 MB | 2018-02-07 | MD5 checksum: c6d4651cd5a4502b048bbdc45a9139d9 |
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Aves: AUGUSTUS HMM search (Cogent) | Mixed archive | TAR | 1.33 MB | 2018-02-07 | MD5 checksum: 9e5bb2a71b6bdbb16546d910ce9e079a |
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Aves: translated proteins (Cogent) | Mixed archive | TAR | 17.02 MB | 2018-02-07 | MD5 checksum: 39add6dc6ac48d2279aac6da51755eae |
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Aves: Full Cogent results table | Text | UNKNOWN | 404.12 kB | 2018-02-07 | MD5 checksum: ea1d378db02ad178af10483aa3c73fd3 |
Funding body | Awardee | Award ID | Comments |
---|---|---|---|
Human Frontier Science Program | Dr. Kenneth C. Welch | RGP0062/2016 | |
Natural Sciences and Engineering | |||
Research Council of Canada | Dr. Kenneth C. Welch | 386466 |
Date | Action |
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February 7, 2018 | Dataset publish |
March 30, 2018 | Manuscript Link added : 10.1093/gigascience/giy009 |
November 10, 2022 | Manuscript Link updated : 10.1093/gigascience/giy009 |