Biological effects of heavy-ion beam irradiation on cyclamen

Masao Sugiyama; Hiroyuki Saito; Hiroyuki Ichida; Yoriko Hayashi; Hiromichi Ryuto; Nobuhisa Fukunishi; Teruhiko Terakawa; Tomoko Abe

doi:10.5511/plantbiotechnology.25.101

New technology to create novel flowers using heavy-ion beam irradiation

Biological effects of heavy-ion beam irradiation on cyclamen

Masao Sugiyama, Hiroyuki Saito, Hiroyuki Ichida, Yoriko Hayashi, Hiromichi Ryuto, Nobuhisa Fukunishi, Teruhiko Terakawa, Tomoko Abe

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Keywords: cyclamen, ion beam, tuber

JOURNAL FREE ACCESS

2008 Volume 25 Issue 1 Pages 101-104

DOI https://doi.org/10.5511/plantbiotechnology.25.101

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Abstract

The biological effects of heavy-ion beam irradiation on cultured tissues of cyclamen were investigated by establishing an irradiation-mediated mutation breeding protocol for producing a new variety of cyclamen. Initially, a callus, a somatic embryo, and a plantlet were irradiated with ¹²C⁶⁺ ion beam at doses of 10, 20, 40, 60, and 80 Gy. Distinct mutants were not obtained in plants regenerated from the irradiated cultured materials. Next, we used the tuber of cyclamen as a target for irradiation. Irradiation (8–16 Gy) of 8–15 mm diameter tubers produced male sterility, change in petal colour, and petal form. Mutation induction by heavy-ion beam irradiation to the tuber is useful for changing flower characteristics of cyclamen.

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