The purpose of this study was to compare the kinematic characteristics of the upper body between baseball tee batting and pitched ball batting in order to gain basic knowledge for improvement of batting skill. Ten male collegiate baseball players (age: 20.7±1.1 yr; height: 1.75±0.05 m; body mass: 76.3±7.1 kg; athletic career: 12.7±2.7 yr) participated. They performed 2 kinds of batting: tee batting (TB) and machine-pitch batting (MB) using a pitching machine (approximate ball speed 33.3 m/s), which were set at middle ball height for the strike zone. Three-dimensional coordinate data were acquired with a motion capture system. Kinematic variables such as maximum bat-head speed, swing time, bat angle, joint angles of the upper limbs, and segment angle of the upper trunk were calculated. Differences between TB and MB were analyzed statistically using paired t-test (p<0.05). The maximum bat-head speed was significantly greater in TB than in MB, but swing times divided into 2 phases showed no significant differences between MB and TB. In the first half of the swing, the bat inclination angle was significantly larger in MB than in TB. The joint angles of the barrel-side shoulder abduction, knob-side shoulder adduction and internal rotation were significantly larger in MB than in TB, and those of the barrel-side shoulder internal rotation and individual elbow pronations were significantly larger in TB than in MB. The clockwise rotational angle of the upper trunk was significantly larger in TB than in MB. In the last half of the swing, the joint angles of the barrel-side shoulder abduction, knob-side shoulder flexion and adduction were significantly larger in MB than in TB, and that of the knob-side elbow pronation was significantly larger in TB than in MB. The changes in upper body movement in MB affected the radius of rotation of the bat about the vertical axis to control the bat easily. The movements in the last half of the swing largely resulted from those in the first half of the swing, and did not contribute to timing adjustment. These results indicate that the initial configuration of the bat and upper limbs, and movements at the beginning of the bat swing contribute to the timing adjustment of the bat for a pitched ball. The results of the present study suggest that it could be useful to pay attention to the movement of the bat and upper body in the first half of the swing as TB practice in order to improve timing adjustment.