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Di (2-Etilhekzil) Fitalatın Ratlarda Lipid Peroksidasyonu ve Antioksidan Düzeylerine Etkisi

Year 2022, Volume: 15 Issue: 1, 51 - 59, 30.04.2022

Merve Eskici Kaymak Emine Altın Ali Ertekin

https://doi.org/10.52976/vansaglik.1008005

Abstract

Bu çalışmada Di (2-Etilheksil) Fitalat (DEHP) maruziyetinin, ratlardan alınan kan örneklerinde lipid peroksidasyonu ve antioksidan seviyeleri üzerine etkileri incelendi. Çalışma için 250-300 gr ağırlığında 40 adet dişi Wistar-Albino rat kullanıldı. 5 gruba ayrılan ratlarda gruplar şu şekilde belirlendi; grup 1: kontrol grubu, grup 2: pozitif kontrol (mısır yağı bazlı diyet), grup 3: 20 mg DEHP/kg canlı ağırlık (c.a.) (test edilen en düşük doz), grup 4: 100 mg DEHP kg/c.a. (orta doz), grup 5: 500 mg DEHP kg/c.a. (test edilen en yüksek doz). 14 günlük deney süresince, ratlar gastrik gavaj ile beslendi. Deney sonunda sakrifiye edilen ratların kalplerinden kan örnekleri alındı. Malondialdehit (MDA), glutatyon (GSH), seruloplazmin, C vitamini ve total protein düzeyleri ölçüldü. DEHP verilen tüm gruplarda GSH seviyeleri azaldı (p <0.05). En düşük GSH düzeyi 100 mg DEHP grubunda gözlendi. MDA, seruloplazmin, C vitamini ve toplam protein seviyelerindeki değişiklikler istatistiksel olarak anlamlı değildi.

Keywords

Antioksidan maddeler C vitamini glutatyon malondialdehit seruloplazmin toplam protein.

Supporting Institution

Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Komisyon Başkanlığı

Project Number

PYO.VET.1904.19.014

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The Effect of Di (2-Ethylhexyl) Phthalate on Lipid Peroxidation and Antioxidant Levels in Rats

Year 2022, Volume: 15 Issue: 1, 51 - 59, 30.04.2022

Merve Eskici Kaymak Emine Altın Ali Ertekin

https://doi.org/10.52976/vansaglik.1008005

Abstract

The effects of Di (2-Ethylhexyl) Phthalate (DEHP) exposure on lipid peroxidation and antioxidant levels in blood samples of rats were investigated. Forty female Wistar-Albino rats weighing 250-300 g were used for the study. The rats were divided into 5 groups; group 1: control group, group 2: positive control (corn oil-based diet), group 3: 20 mg DEHP per kg body weight (bw) (lowest dose tested), group 4: 100 mg DEHP kg/bw (medium dose tested), group 5: 500 mg DEHP kg/bw (highest dose tested). During the 14-day experiment, the rats were fed by gastric gavage. At the end of the experiment, the rats were sacrificed, and blood samples were taken from the heart of the rats. The levels of glutathione (GSH), malondialdehyde (MDA), ceruloplasmin, vitamin C, and total protein were measured. GSH levels were reduced in all groups that received DEHP (p <0.05). The lowest level of GSH was observed in the 100 mg DEHP group. The changes within the levels of MDA, ceruloplasmin, vitamin C and total proteins weren't statistically significant.

Keywords

Antioxidant substances ceruloplasmin glutathione malondialdehyde total protein vitamin C.

Project Number

PYO.VET.1904.19.014

References

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  • Buckley, Jessie P., Lesliam Quirós-Alcalá, Susan L. Teitelbaum, Antonia M. Calafat, Mary S. Wolff, and Stephanie M. Engel. 2018. “Associations of Prenatal Environmental Phenol and Phthalate Biomarkers with Respiratory and Allergic Diseases among Children Aged 6 and 7 years.” Environment International 115:79–88. doi: 10.1016/j.envint.2018.03.016.
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  • David, R. M., M. R. Moore, D. C. Finney, and D. Guest. 2000. “Chronic Toxicity of Di(2-Ethylhexyl)Phthalate in Mice.” Toxicological Sciences : An Official Journal of the Society of Toxicology 58(2):377–85. doi: 10.1093/toxsci/58.2.377.
  • Durusoy Onmuş, İsabel Raika, and Ali Osman Karababa. 2011. “Plastik Gıda Ambalajları ve Sağlık.” TSK Koruyucu Hekimlik Bülteni 10(1):87–96.
  • Erkekoglu, Pınar, Naciye D. Zeybek, Belma K. Giray, Walid Rachidi, Murat Kızılgün, Isabelle Hininger-Favier, Alain Favier, Esin Asan, and Filiz Hincal. 2014. “The Effects of Di(2-Ethylhexyl)Phthalate on Rat Liver in Relation to Selenium Status.” International Journal of Experimental Pathology 95(1):64–77. doi: 10.1111/iep.12059.
  • Ferguson, Kelly K., Rita Loch-Caruso, and John D. Meeker. 2011. “Urinary Phthalate Metabolites in Relation to Biomarkers of Inflammation and Oxidative Stress: NHANES 1999-2006.” Environmental Research 111(5):718–26. doi: 10.1016/j.envres.2011.02.002.
  • Foresta, Carlo, Andrea Garolla, Daniela Zuccarello, Damiano Pizzol, Afra Moretti, Luisa Barzon, and Giorgio Palù. 2010. “Human Papillomavirus Found in Sperm Head of Young Adult Males Affects the Progressive Motility.” Fertility and Sterility 93(3):802–6. doi: 10.1016/j.fertnstert.2008.10.050.
  • Gill, Kamalpreet Kaur, Harpal Singh Sandhu, and Rajdeep Kaur. 2015. “Evaluation of Lipid Peroxidation and Antioxidant Status on Fenvalerate, Nitrate and Their Co-Exposure in Bubalus Bubalis.” Pesticide Biochemistry and Physiology 123:19–23. doi: 10.1016/j.pestbp.2015.01.013.
  • Göktekin, Emre. 2016. “Prenatal Dönemde Dihekzil Fitalat ve Disiklohekzil Fitalata Maruziyetin Erkek ve Dişi Sıçanların Bazı Endokrin Dokuları Üzerindeki Etkilerinin Incelenmesi (in Turkish).” Hacettepe University, Graduate School of Science and Engineering, Department of Biology, PhD. Thesis, Ankara, Turkey.
  • Heudorf, Ursel, Volker Mersch-Sundermann, and Jürgen Angerer. 2007. “Phthalates: Toxicology and Exposure.” International Journal of Hygiene and Environmental Health 210(5):623–34. doi: 10.1016/j.ijheh.2007.07.011.
  • Høyer, Birgit Bjerre, Virissa Lenters, Aleksander Giwercman, Bo A. G. Jönsson, Gunnar Toft, Karin S. Hougaard, Jens Peter E. Bonde, and Ina Olmer Specht. 2018. “Impact of Di-2-Ethylhexyl Phthalate Metabolites on Male Reproductive Function: A Systematic Review of Human Evidence.” Current Environmental Health Reports 5(1):20–33. doi: 10.1007/s40572-018-0174-3.
  • İmren, Ceren. 2011. “Endokrin Bozucu Dimetil Fitalatın Sülfat Radikali Bazlı Fotokimyasal İleri Oksidasyon Prosesleri İle Gideriminin Araştırılması (in Turkish).” İstanbul Technical University, Institute of Science and Technology, M.Sc., İstanbul, Turkey. Jacob, Robert A., and Gity Sotoudeh. 2002. “Vitamin C Function and Status in Chronic Disease.” Nutrition in Clinical Care : An Official Publication of Tufts University 5(2):66–74.
  • Jain, S. K., R. McVie, J. Duett, and J. J. Herbst. 1989. “Erythrocyte Membrane Lipid Peroxidation and Glycosylated Hemoglobin in Diabetes.” Diabetes 38(12):1539–43. doi: 10.2337/diab.38.12.1539.
  • John, Peter W. M. 1971. Statistical Design and Analysis of Experiments. New York: Macmillan Co.
  • Johns, Lauren E., Glinda S. Cooper, Audrey Galizia, and John D. Meeker. 2015. “Exposure Assessment Issues in Epidemiology Studies of Phthalates.” Environment International 85:27–39. doi: 10.1016/j.envint.2015.08.005.
  • Kasahara, Emiko, Eisuke F. Sato, Mami Miyoshi, Ryusei Konaka, Keiichi Hiramoto, Junzo Sasaki, Masaaki Tokuda, Yoshihisa Nakano, and Masayasu Inoue. 2002. “Role of Oxidative Stress in Germ Cell Apoptosis Induced by Di(2-Ethylhexyl)Phthalate.” Biochemical Journal 365(3):849–56. doi: 10.1042/BJ20020254.
  • Kay, Vanessa R., Christina Chambers, and Warren G. Foster. 2013. “Reproductive and Developmental Effects of Phthalate Diesters in Females.” Critical Reviews in Toxicology 43(3):200–219. doi: 10.3109/10408444.2013.766149.
  • Kessler, Winfried, Wanwiwa Numtip, Wolfgang Völkel, Elcim Seckin, György A. Csanády, Christian Pütz, Dominik Klein, Hermann Fromme, and Johannes G. Filser. 2012. “Kinetics of Di(2-Ethylhexyl) Phthalate (DEHP) and Mono(2-Ethylhexyl) Phthalate in Blood and of DEHP Metabolites in Urine of Male Volunteers after Single Ingestion of Ring-Deuterated DEHP.” Toxicology and Applied Pharmacology 264(2):284–91. doi: 10.1016/j.taap.2012.08.009.
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There are 51 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Original Research Articles
Authors

Merve Eskici Kaymak 0000-0002-8836-7987

Emine Altın 0000-0002-3310-3044

Ali Ertekin 0000-0002-6299-9716

Project Number PYO.VET.1904.19.014
Publication Date April 30, 2022
Submission Date October 11, 2021
Published in Issue Year 2022 Volume: 15 Issue: 1

Cite

APA Eskici Kaymak, M., Altın, E., & Ertekin, A. (2022). The Effect of Di (2-Ethylhexyl) Phthalate on Lipid Peroxidation and Antioxidant Levels in Rats. Van Sağlık Bilimleri Dergisi, 15(1), 51-59. https://doi.org/10.52976/vansaglik.1008005
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