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In vitro evaluation of the protective effects of Curcumin and Resveratrol against U87 Cells Induced by Beta-Amyloid

Year 2022, Volume: 11 Issue: 3, 304 - 311, 30.12.2022
https://doi.org/10.47493/abantmedj.1123424

Abstract

Objective: In this study we aimed to investigate the effects of curcumin and resveratrol antioxidant enzymes in β-amyloid (Aβ)-induced in vitro Alzheimer's Disease (AD) cell model.
Materials and Methods: Three groups were created in this study; The control group consisted of U87 cells, the Aβ group which was the in vitro AD model formed from the β-amyloid-induced U-87 cell lines, and the Aβ+ Curcumin+ Resveratrol group by adding curcumin and resveratrol to the Aβ group. Cell viability in groups was evaluated with the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test. Total Antioxidant Level (TAS), Superoxide dismutase (SOD), Total Oxidant Level (TOS), and Catalase (CAT) enzyme levels, results were analyzed in order to evaluate the antioxidant levels.
Results: When cell viability was evaluated, it was determined that curcumin and resveratrol did not have cytotoxic effects. TAS levels were statistically higher in the Aβ+ curcumin and resveratrol group compared to the Aβ group (p<0.05) and TOS levels were found to be significantly low in the Aβ+ curcumin and resveratrol group compared to the Aβ group (p<0.05). However, when compared to the control group, CAT and SOD enzyme levels were statistically and significantly low in the Aß group. In contrast, these enzyme levels were found significantly higher in the Aβ+ curcumin and resveratrol group in comparison with the Aß group (p<0.05).
Conclusion: In conclusion, it has been shown that curcumin and resveratrol support antioxidant activity.

References

  • 1. Dinamarca MC, Cerpa W, Garrido J, Hancke JL, Inestrosa NC. Hyperforinprevents beta-amyloidneurotoxicity and spatial memory impairments by disaggregation of Alzheimer's amyloid-beta-deposits. MolPsychiatry, 2006, 11(11):1032--1048.
  • 2. Dos Santos Picanco, LC, Ozela PF, Fatima de Brito Brito M, Pinheiro AA. Padilha E. C, Braga FS Silva Hage-Melim, LI. Alzheimer's disease: A review from the pathophysiology to diagnosis, new perspectives for pharmacological treatment. Current medicinal chemistry. 2018; 25 (26): 3141-3159
  • 3. Gol M, Ghorbanian D, Soltanpour N, Faraji J, Pourghasem M. Protective effect of raisin (currant) against spatial memory impairment and oxidative stress in alzheimer disease model. Nutritional neuroscience. 2019; 22 (2): 110-118.
  • 4. Baluchnejadmojarad T, Mohamadi-Zarch SM, Roghani M. Safranal, an active ingredient of saffron, attenuates cognitive deficits in amyloid β-induced rat model of alzheimer’s disease: Underlying mechanisms. Metabolic Brain Disease. 2019; 34 (6): 1747-1759.
  • 5. Topçuoğlu ES, Selekler K. Alzheimer disease. Turkish Journal of Geriatrics, 1998;1(2), 63-7.
  • 6. A. Baur, D.A. Sinclair.Therapeutic potential of resveratrol: the in vivo evidence Nat Rev Drug Discov, 5 (2006), pp. 493-506
  • 7. Takaoka, M. (1940). The phenolic substances of white hellebore (Veratrum Grandiflorum Loes. Fill). V. Nippon Kagaku Kaishi 61.
  • 8. Tadolini, B., Juliano, C., Piu, L., Franconi, F., and Cabrini, L. (2000). Resveratrol inhibition of lipid peroxidation. Free Radic. Res. 33, 105–114. doi: 10.1080/10715760000300661.
  • 9. J. A. Baur and D. A. Sinclair, “Therapeutic potential of resveratrol: the in vivo evidence,” Nature Reviews Drug Discovery, vol. 5, no. 6, pp. 493–506, 2006.Picciano AP. Vaden TD. Complexation between Cu(II) and curcumin in the presence of two different segments of amyloid β. Biophysical Chemistry.2013; 184, 62--67.
  • 10. JA. Baur and D. A. Sinclair, “Therapeutic potential of resveratrol: the in vivo evidence,” Nature Reviews Drug Discovery, vol. 5, no. 6, pp. 493–506, 2006.Picciano AP. Vaden TD. Complexation between Cu (II) and curcumin in the presence of two different segments of amyloid β. Biophysical Chemistry.2013; 184, 62--67.
  • 11. Shankar GM, Walsh, DM. Alzheimer's disease: Synaptic dysfunction and abeta. Molecular neurodegeneration. 2009; 4: 48. 12. Gol M, Ghorbanian D, Soltanpour N, Faraji J, Pourghasem M. Protective effect of raisin (currant) against spatial memory impairment and oxidative stress in alzheimer disease model. Nutritional neuroscience. 2019; 22 (2): 110-118.
  • 13. Sherki YG, Melamed E & Offen D. Oxidative Stress İnduced-Neurodegenerative Diseases: The Need For Antioxidants That Penetrate The Blood Brain Barrier. Neuropharmacology. 2001. 40(8): 959–975, PMID: 11406187
  • 14. Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin: The Indian solid gold. Adv Exp Med Biol 2007; 595:1–75.
  • 15. Youssef KM, El-Sherbeny MA. Synthesis and antitumor activity of some curcumin analogs. Arch Pharm (Weinheim) 2005; 338:181–9
  • 16. Lin C.W., Yang L.Y., Shen S.C., Chen Y.C. IGF-I plus E2 induces proliferation via activation of ROS-dependent ERKs and JNKs in human breast carcinoma cells. J Cell Physiol, 2007; 212(3): 666-74.
  • 17. Kılınç E, Var A, Mavioğu H, et al. Cırculatıng Manganese Superoxıde Dısmutase (Mn-SOD, SOD2) Levels And Its Ala16val Polymorphısm In Patıents Wıth Alzheımer’s Dısease. Turkish Journal Of Geriatrics. 2012. 15(1): 1-6.
  • 18. Altıner A. Atalay H, Bilal T. Serbest Radikaller ve Stres İle İlişkisi. Balıkesir Sağlık Bilimleri Dergisi. 2017. 7 (1):51-55, DOİ: 10.5505/bsbd.2018.38243
  • 19. Sherki YG, Melamed E, Offen D. Oxidative Stress İnduced-Neurodegenerative Diseases: The Need For Antioxidants That Penetrate The Blood Brain Barrier. Neuropharmacology. 2001. 40(8): 959–975, PMID: 11406187.
  • 20. Joanna T. Maciejczy M, Konopka T, Zalewska A. Total Oxidant and Antioxidant Capacity of Gingival Crevicular Fluid and Saliva in Patients with Periodontitis: Review and Clinical Study, Antioxidants 2020, 9(5), 450.
  • 21. Rong W. Feng J. Yang Y. Dai C. Lu A. et al., Significance of Serum Total Oxidant/Antioxidant Status in Patients with Colorectal Cancer, 2017.
  • 22. El-Edel Rawhia H, Abou-Elela Dalia H, Sonbol Ahmed A, Shalaby Amr S, Fouaad Mariam , Total antioxidant capacity as an aging marker in Egyptian patients with Alzheimer disease, A: 2020; 33 (3): 914-919.

Beta-Amiloid Tarafından İndüklenen U87 Hücrelerine Karşı Kurkumin ve Resveratrol'ün koruyucu etkilerinin in vitro değerlendirilmesi

Year 2022, Volume: 11 Issue: 3, 304 - 311, 30.12.2022
https://doi.org/10.47493/abantmedj.1123424

Abstract

Amaç: Çalışmamız, β-amiloid (Aβ) ile indüklenen U87 hücrelerin in vitro Alzheimer Hastalığı (AH) modeli olarak kurkumin ve resveratrol’un antioksidan enzimler üzerindeki etkilerini araştırmak amacıyla yapılmıştır.
Gereç ve Yöntemler: Bu çalışmada; U87 hücrelerden oluşan kontrol grubu, β-amiloid ile indüklenen U-87 hücre dizilerini içeren in vitro AD modelinin oluşturulduğu Aβ grubu ve Aβ grubuna kurkumin ve resveratrol eklenerek Aβ+ Curcumin+ Resveratrol grubu olmak üzere üç grup oluşturuldu. Grupların hücre canlılığı 3-(4,5-dimetiltiazol-2-il)-2, 5-difeniltetrazolyum bromür (MTT) testi ile değerlendirildi. Total Antioksidan Seviyesi (TAS), Süperoksit dismutaz (SOD), Total Oksidan Seviyesi (TOS) ve Katalaz (CAT) enzim seviyeleri, antioksidan durumunu değerlendirmek için sonuçlar analiz edildi.
Bulgular: Hücre canlılığı değerlendirildiğinde kurkumin ve resveratrolün sitotoksik etkilerinin olmadığı belirlendi. Aβ+ kurkumin ve resveratrol grubunda Aβ grubuna göre TAS düzeyleri istatistiksel olarak daha yüksek (p<0,05) ve TOS düzeyleri Aβ+ kurkumin ve resveratrol grubunda Aβ grubuna göre istatistiksel olarak anlamlı derecede düşük bulundu(p<0,05). Ancak kontrol grubu ile karşılaştırıldığında Aß grubunda CAT ve SOD enzim düzeyleri istatistiksel olarak anlamlı düzeyde düşük bulunurken, bu enzim düzeyleri Aβ+ kurkumin ve resveratrol grubunda Aß grubuna göre istatistiksel olarak anlamlı düzeyde yüksek bulundu. (p<0,05).
Sonuç: Sonuç olarak, kurkumin ve resveratrolün apoptoza bağlı nörodejenerasyonu azalttığı ve antioksidan aktiviteyi desteklediği gösterilmiştir.

References

  • 1. Dinamarca MC, Cerpa W, Garrido J, Hancke JL, Inestrosa NC. Hyperforinprevents beta-amyloidneurotoxicity and spatial memory impairments by disaggregation of Alzheimer's amyloid-beta-deposits. MolPsychiatry, 2006, 11(11):1032--1048.
  • 2. Dos Santos Picanco, LC, Ozela PF, Fatima de Brito Brito M, Pinheiro AA. Padilha E. C, Braga FS Silva Hage-Melim, LI. Alzheimer's disease: A review from the pathophysiology to diagnosis, new perspectives for pharmacological treatment. Current medicinal chemistry. 2018; 25 (26): 3141-3159
  • 3. Gol M, Ghorbanian D, Soltanpour N, Faraji J, Pourghasem M. Protective effect of raisin (currant) against spatial memory impairment and oxidative stress in alzheimer disease model. Nutritional neuroscience. 2019; 22 (2): 110-118.
  • 4. Baluchnejadmojarad T, Mohamadi-Zarch SM, Roghani M. Safranal, an active ingredient of saffron, attenuates cognitive deficits in amyloid β-induced rat model of alzheimer’s disease: Underlying mechanisms. Metabolic Brain Disease. 2019; 34 (6): 1747-1759.
  • 5. Topçuoğlu ES, Selekler K. Alzheimer disease. Turkish Journal of Geriatrics, 1998;1(2), 63-7.
  • 6. A. Baur, D.A. Sinclair.Therapeutic potential of resveratrol: the in vivo evidence Nat Rev Drug Discov, 5 (2006), pp. 493-506
  • 7. Takaoka, M. (1940). The phenolic substances of white hellebore (Veratrum Grandiflorum Loes. Fill). V. Nippon Kagaku Kaishi 61.
  • 8. Tadolini, B., Juliano, C., Piu, L., Franconi, F., and Cabrini, L. (2000). Resveratrol inhibition of lipid peroxidation. Free Radic. Res. 33, 105–114. doi: 10.1080/10715760000300661.
  • 9. J. A. Baur and D. A. Sinclair, “Therapeutic potential of resveratrol: the in vivo evidence,” Nature Reviews Drug Discovery, vol. 5, no. 6, pp. 493–506, 2006.Picciano AP. Vaden TD. Complexation between Cu(II) and curcumin in the presence of two different segments of amyloid β. Biophysical Chemistry.2013; 184, 62--67.
  • 10. JA. Baur and D. A. Sinclair, “Therapeutic potential of resveratrol: the in vivo evidence,” Nature Reviews Drug Discovery, vol. 5, no. 6, pp. 493–506, 2006.Picciano AP. Vaden TD. Complexation between Cu (II) and curcumin in the presence of two different segments of amyloid β. Biophysical Chemistry.2013; 184, 62--67.
  • 11. Shankar GM, Walsh, DM. Alzheimer's disease: Synaptic dysfunction and abeta. Molecular neurodegeneration. 2009; 4: 48. 12. Gol M, Ghorbanian D, Soltanpour N, Faraji J, Pourghasem M. Protective effect of raisin (currant) against spatial memory impairment and oxidative stress in alzheimer disease model. Nutritional neuroscience. 2019; 22 (2): 110-118.
  • 13. Sherki YG, Melamed E & Offen D. Oxidative Stress İnduced-Neurodegenerative Diseases: The Need For Antioxidants That Penetrate The Blood Brain Barrier. Neuropharmacology. 2001. 40(8): 959–975, PMID: 11406187
  • 14. Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin: The Indian solid gold. Adv Exp Med Biol 2007; 595:1–75.
  • 15. Youssef KM, El-Sherbeny MA. Synthesis and antitumor activity of some curcumin analogs. Arch Pharm (Weinheim) 2005; 338:181–9
  • 16. Lin C.W., Yang L.Y., Shen S.C., Chen Y.C. IGF-I plus E2 induces proliferation via activation of ROS-dependent ERKs and JNKs in human breast carcinoma cells. J Cell Physiol, 2007; 212(3): 666-74.
  • 17. Kılınç E, Var A, Mavioğu H, et al. Cırculatıng Manganese Superoxıde Dısmutase (Mn-SOD, SOD2) Levels And Its Ala16val Polymorphısm In Patıents Wıth Alzheımer’s Dısease. Turkish Journal Of Geriatrics. 2012. 15(1): 1-6.
  • 18. Altıner A. Atalay H, Bilal T. Serbest Radikaller ve Stres İle İlişkisi. Balıkesir Sağlık Bilimleri Dergisi. 2017. 7 (1):51-55, DOİ: 10.5505/bsbd.2018.38243
  • 19. Sherki YG, Melamed E, Offen D. Oxidative Stress İnduced-Neurodegenerative Diseases: The Need For Antioxidants That Penetrate The Blood Brain Barrier. Neuropharmacology. 2001. 40(8): 959–975, PMID: 11406187.
  • 20. Joanna T. Maciejczy M, Konopka T, Zalewska A. Total Oxidant and Antioxidant Capacity of Gingival Crevicular Fluid and Saliva in Patients with Periodontitis: Review and Clinical Study, Antioxidants 2020, 9(5), 450.
  • 21. Rong W. Feng J. Yang Y. Dai C. Lu A. et al., Significance of Serum Total Oxidant/Antioxidant Status in Patients with Colorectal Cancer, 2017.
  • 22. El-Edel Rawhia H, Abou-Elela Dalia H, Sonbol Ahmed A, Shalaby Amr S, Fouaad Mariam , Total antioxidant capacity as an aging marker in Egyptian patients with Alzheimer disease, A: 2020; 33 (3): 914-919.
There are 21 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Articles
Authors

Ares Alizade 0000-0003-0334-8152

Gülüzar Özbolat 0000-0002-3487-1088

Publication Date December 30, 2022
Submission Date May 30, 2022
Published in Issue Year 2022 Volume: 11 Issue: 3

Cite

APA Alizade, A., & Özbolat, G. (2022). In vitro evaluation of the protective effects of Curcumin and Resveratrol against U87 Cells Induced by Beta-Amyloid. Abant Medical Journal, 11(3), 304-311. https://doi.org/10.47493/abantmedj.1123424
AMA Alizade A, Özbolat G. In vitro evaluation of the protective effects of Curcumin and Resveratrol against U87 Cells Induced by Beta-Amyloid. Abant Med J. December 2022;11(3):304-311. doi:10.47493/abantmedj.1123424
Chicago Alizade, Ares, and Gülüzar Özbolat. “In Vitro Evaluation of the Protective Effects of Curcumin and Resveratrol Against U87 Cells Induced by Beta-Amyloid”. Abant Medical Journal 11, no. 3 (December 2022): 304-11. https://doi.org/10.47493/abantmedj.1123424.
EndNote Alizade A, Özbolat G (December 1, 2022) In vitro evaluation of the protective effects of Curcumin and Resveratrol against U87 Cells Induced by Beta-Amyloid. Abant Medical Journal 11 3 304–311.
IEEE A. Alizade and G. Özbolat, “In vitro evaluation of the protective effects of Curcumin and Resveratrol against U87 Cells Induced by Beta-Amyloid”, Abant Med J, vol. 11, no. 3, pp. 304–311, 2022, doi: 10.47493/abantmedj.1123424.
ISNAD Alizade, Ares - Özbolat, Gülüzar. “In Vitro Evaluation of the Protective Effects of Curcumin and Resveratrol Against U87 Cells Induced by Beta-Amyloid”. Abant Medical Journal 11/3 (December 2022), 304-311. https://doi.org/10.47493/abantmedj.1123424.
JAMA Alizade A, Özbolat G. In vitro evaluation of the protective effects of Curcumin and Resveratrol against U87 Cells Induced by Beta-Amyloid. Abant Med J. 2022;11:304–311.
MLA Alizade, Ares and Gülüzar Özbolat. “In Vitro Evaluation of the Protective Effects of Curcumin and Resveratrol Against U87 Cells Induced by Beta-Amyloid”. Abant Medical Journal, vol. 11, no. 3, 2022, pp. 304-11, doi:10.47493/abantmedj.1123424.
Vancouver Alizade A, Özbolat G. In vitro evaluation of the protective effects of Curcumin and Resveratrol against U87 Cells Induced by Beta-Amyloid. Abant Med J. 2022;11(3):304-11.