13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

-  Poster 14

TITLE: Analysis of keratinocytes Proliferation During Repair of Surgical Incisions Performed by Conventional Scapel, Electro Cautery, CO₂ and Diode Laser.

AUTHORS: Teixeira LR, Teixeira BC, Aranha ACC, Viera MF, Corrêa L, Azevedo LH.

USP - LELO – IPEN

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S162.

* doi:10.4317/medoral.17643662

http://dx.doi.org/10.4317/medoral.17643662

Abstract

Carbon dioxide CO₂ laser and high energy diode laser are extensively applied for oral surgery procedures, but the impact on the tissue repair of tissue ablation and thermal injury caused by these lasers is not well-established. The aim of this study was to compare the cell index proliferation through proliferating cell nuclear antigen (PCNA) immunolabeling on the epithelium of incisions induced in the rat tongue. Standardized linear incisions were performed by conventional scalpel, electro cautery, CO₂ laser (2W), CO₂ (4W), diode laser (2W), and diode laser (4W). Immunohistochemical tests using anti-PCNA antibody were performed for keratinocytes proliferation analysis at 0h and 1, 2, 3, 7 and 14 days after surgical incisions. PCNA-positive cells and the epithelium thickness area in the incisions borders were histomorphometrically measured. In general, the CO₂ lasers groups (2W and 4W) had higher number of positive cells than diode lasers groups (2W and 4W), but this number wa s lower than conventional scalpel and electro cautery. From 3 days to 14 days, the number of positive cells was increasing in diode laser groups, but higher than conventional scalpel and electro cautery. In conclusion, CO₂ lasers seem to cause thermal damage that interferes with keratinocytes proliferation, but in lower level than diode lasers. Both lasers show cell proliferation indexes lower than conventional scalpel and electro cautery.