Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
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Examination of conditions for regular internal quality control in identification of microorganisms using MALDI-TOF MS
KAZUYUKI SOGAWA, MAKIKO KIYOSUKE, KANAKO HATTORI, SYOTA MURATA, TOMOHISA WATARI, KOJI KUSABA, YUJI SAEKI, KAZUKI HORIUCHI, KAZUNARI YASUDA, KOHEI UECHI, HAJIME OKUMURA, SACHIE SEKIGUCHI, AZUMI FUJINAGA, MASAMI MURAKAMI, TOMOHIRO NAKAYAMA
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Keywords: Identification of microorganisms, Internal quality control, Matrix-assisted laser desorption, ionization time-of-flight mass spectrometry
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2021 Volume 26 Issue 2 Pages 99-104

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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was approved for medical use in 2011, and is currently used as a rapid, accurate and low-cost technique for bacterial identification. Microbiological testing and internal accuracy control in Japan are mainly implemented in accordance with the standards of the Clinical and Laboratory Standards Institute (CLSI). However, few facilities perform internal accuracy control of bacterial identification by MALDI-TOF MS. Therefore, we examined the procedures for internal accuracy control of bacterial identification using MALDI-TOF MS in daily work at clinical laboratories in the seven hospitals.

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© 2021 The Society for Antibacterial and Antifungal Agents, Japan
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