Biocontrol Science
Online ISSN : 1884-0205
Print ISSN : 1342-4815
ISSN-L : 1342-4815
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Thermal Death of Bacillus subtilis Spores in Oil-Water Systems
MAYUMI SHIGEMOTOKAYO NAKAGAWAJIN J. SAKAMOTOTETSUAKI TSUCHIDO
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2010 Volume 15 Issue 1 Pages 27-31

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Abstract

The thermal death of the spores of Bacillus subtilis 168 in oil-water systems including emulsions and separated layers consisting of phosphate buffer and soybean oil or n-hexadecane was investigated. The resultant survivor curve consisted of two phases, an initial rapid reduction followed by a slow reduction, possibly as reflected by the death in the water phase and the oil phase, respectively. The concentration of oil in the system strikingly affected the pattern of thermal death. These results suggest that the spore location in the oil-water system may be a critical factor in determining the heat resistance.

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© 2010 The Society for Antibacterial and Antifungal Agents, Japan
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