A practical method for the preparation of total DNA from filamentous fungi

Authors

M. I. BORGES, Universidade de Brasilia
M. O. AZEVEDO, Universidade de Brasilia
R. BONATELLI JR., Universidade de Brasilia
M. S. S. FELIPE, Universidade de Brasilia
S. ASTOLFI- FILHO, Universidade de Brasilia

Abstract

Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chloride, or suitable only for small scale preparations. We have developed a simple method for total DNA preparation, yielding a product of quality suitable for restriction digestion and library construction.

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Recommended Citation

BORGES, M. I., M.O. AZEVEDO, R. BONATELLI, M.S. FELIPE, and S. ASTOLFI- FILHO (1990) "A practical method for the preparation of total DNA from filamentous fungi," Fungal Genetics Reports: Vol. 37, Article 1. https://doi.org/10.4148/1941-4765.1466